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  2. McMahon Group techniques

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/techniqs.html
    26 Sep 2011: Overexpression of endocytic proteins in COS cells to test the effects of mutations in vivo. ... Liposome/lipid nanotube sedimentation assays to test lipid binding and specificity. (also immunoprecipitations, cloning, kinase assays, etc.).
  3. Endocytic Interactome: How Interactomes can help us design…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/Interactomes/index.html
    26 Sep 2011: What is endophilin and how does it work. Research on the mechanism of endophilin in rapid vesicle endocytosis. Research on proteins involved in clathrin-coated vesicle formation. Research on membrane bending. Research on protein-lipid interactions.
  4. Clathrin and its interactions with AP180. AP180 is a brain enriched…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/AP180/Clathrin.html
    26 Sep 2011: More experiments need to be done to test this. The platinum shadowing (from a single angle) shows that AP180 does not induce curvature in clathrin assemblies (left panel).
  5. YopJ activation by IP6

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/Black_Plague/YopJ_IP6.html
    26 Sep 2011: Researching the reason immune responses are sprroessed during Yersinia infections.
  6. Isothermal titration calorimetry to measure protein/peptide/lipid…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/ITC.html
    26 Sep 2011: Isothermal Titration Calorimetry is used to make accurate measurements of affinities and stoichiometries of interactions. It is based on fine measurements of heat changes when ligands interact
  7. Volume 22 May 15, 2011 1625 Cytosolic aggregates perturb ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/69_Chakrabarti_O_MBC_2011.pdf
    11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated.
  8. Molecular Biology of the CellVol. 21, 3054 –3069, September ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/RVS161_MBC2010.pdf
    21 Feb 2011: To test these activities, wecoexpressed full-length Rvs161 and Rvs167 using an E. ... To-gether, our biochemical tests show that Rvs161-Rvs167 bindsto membranes and induces three-dimensional curvature.
  9. Protein-driven membrane stresses in fusion and fission

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovCurvature2010.pdf
    21 Feb 2011: middle of the stalk and contributes similarly to theenergies of both fusion and fission stalks [3,73].[(Box_1)TD$FIG]. ... Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test.
  10. Membrane Protein Insertion at the Endoplasmic Reticulum

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/75_Shao_S_Ann_Rev_2011.pdf
    9 Nov 2011: The apparentfree-energy contribution of each amino acidin any test sequence was found to be additive,with the sum determining the final insertionprobability. ... earlier transmembrane domains ( gray), and a later test sequence(red ) is measured for
  11. JCB: Article The Rockefeller University Press $30.00J. Cell Biol. ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/J%20Cell%20Biol%202010%20Howes.pdf
    24 Aug 2011: A single section of the original tomogram is shown (left). Various rotations of a 3D contoured electron density render were generated (middle). ... 6 F). To test the requirement for CLIC endocytosis during mi-gration, this inactivation assay was applied

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