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19 Nov 2004: sZ-N. TmFt. sZ-C. TmFt. sZ-N. C. TmFtsZ TmFtsZ-N. TmFtsZ-C TmFtsZ-NC. Wavelength (nm)200 210 220 230 240 250 260. ... 081% (w/v) malachite green and 2 ml of water were mixed and 200 µl was added to 50-µl reactions contain-ing proteins or inorganic

15 Mar 2004: Crash Cost (£). a none 32 200 26 500 b none 21 300 20 375 c none 30 200 30 200 d a 45 500 40 800 e a,b 26

12 Jan 2004: 25,786 — 25,786 (25,786) — — — — — 200 498 — 498 (698) (200) — — — —. 181,449 13,489 — 13,489 (100,791) (87,302) — (16) 94,131 94,115 306,882 24,092 — ... Keeping Fund — — — — — —Ursula Zoellner Cancer Research

30 Jun 2004: Spo0J] (mM)0 25 50 100 200 300 25 50 100 200 300. ... The protein was buffer exchanged into20 mM Tris, pH 8.5, 200 mM NaCl using a Sephadex G25 gelfiltration column (Amersham).

21 Jul 2004: 23 £13,374 150 - 200 4 CS3. 22 £12,984 15 3 M3. ... 18 £11,536 11 Grade 1:. 17 £11,200 10 CS2. 16 £10,874 9 M1.

21 Dec 2004: Unless specifically noted otherwise, transfections with Lipo-fectamine 2000 (Invitrogen, Carlsbad, CA) were performed in 96-well plateswith a total of 200 ng of plasmid DNA per well. ... Of this 200 ng, the luciferasereporter plasmid was always kept

17 Mar 2004: J. Cell Biol. 155,193-200.

29 Dec 2004: Since K-252a is a much more potent kinase inhibitor than H-9, and some non- specific effects were noted at concentrations of H-9 in excess of 200 pM, most ... Aliquots of cpt-CAMP-treated cells were further treated for 10 min with K-252a (20 PM) or H-9

16 Aug 2004: Tris (pH 7.5),200 mM NaCl and 5 mM MgCl2 and eluted separately.Following calibration, 10 ml of ZapA protein at 10 mg/ml was injected and eluted as a ... 1 mM EDTA,1 mM NaN3 and 200 mM NaCl was placed in thesample compartment of an Epon double-sector

3 Dec 2004: interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al.