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19 Jan 2017: endogenous proteins with recombinant variantsrevealed the functional roles for all of the factorsand interactions. ... C) Immunoblots (IB) for triage components of control or BAG6 complex-depleted RRL replenished with recombinant BAG6 sub-complexes used

15 Nov 2011: To evaluatePtdIns(4,5)P2 content in the plasma membrane and to competefor PtdIns(4,5)P2 binding with endogenous PtdIns(4,5)P2-bindingproteins, we expressed PLCδ1PH (PH domain of ... Lowering the concentrations of PtdIns(4,5)P2available for interactions

14 Jun 2007: How do various ESCRT components link to other processes such as pH regulation of gene expression [51] or retrovirus budding [52]?

26 Aug 2021: Othersubunits of the 19S base were copurified atlower levels, consistent with partial assemblyof PSMC5 with endogenous base subunits pres-ent inthe lysate (table S5). ... Boosting HERC1 levelsusing CRISPR activation (31) of the endoge-nous HERC1 promoter

3 Sep 2014: coli ETEC H10407 was investigated. The protein at endogenous expression levels is easilydetected and this signal disappears in a leoA KO strain, demonstrating specificity.

27 Nov 2003: Immunofluorescence microscopy of endogenous Mbl in fixed cells gave. similar images.

16 Feb 2010: 2007). To maximize the severity of any phenotype, PrP+/ mice were used because endogenous PrP was observed in earlier studies to exacerbate CtmPrP-mediated neurodegeneration (Stewart et al., 2005).

16 Jul 2008: SDS-PAGE, andimmunoblotted using an antibody (3F4) that specifically detects transfected but not endogenous PrP. ... C) Biosynthesisand maturation of endogenous PrP in A4 and L-cells were assessedby pulse-chase analysis followed by immunoprecipitation

8 Nov 2004: Endogenous NF-kB (indicated by an aster-isk) serves as a loading control. ... Immunoblots were probed with antibodiesagainst PrP, GFP, and an endogenous protein, the beta subunit ofSec61.

24 Aug 2011: By Western blot analysis both endogenous and transfected Flot1 and GRAF1 were concentrated in Fraction 2.8 (Fig. ... 4 G, arrowheads). Endogenous dynamin labeled a significant population of struc-tures within Fraction 2.8 (Fig.