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Madeline A. Lancaster Culturing feeder-free human ES cells Modified…
https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Culturing-feeder-free-human-ES-cells.docx21 Mar 2014: 4. 24-48 hours later, transfer to liquid N2. Freezing ES cells using mFresr. ... 4. 24-48 hours later, transfer to liquid N2. -
Madeline A. Lancaster Culturing feeder-dependent human iPS/ES cells…
https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Culturing-human-ES-and-iPS-cells.docx21 Mar 2014: 4. Place in Mr. Frosty in -80C. 5. Move to liquid N2 after 24-48 hrs. ... 5. 24-48 hours later, transfer to liquid N2. Manual passaging hES/hiPS.
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