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https://supfam.mrc-lmb.cam.ac.uk/bioinformatics/people/julian_gough/publications/zeng_molplant_2008.pdf2 Sep 2008: for test. These proteins were expressed with GST-tags in. Escherichia coli and tested for E3 ligase activity using an in-. ... proteins. Nevertheless, such preference could make it difficult. to test their in-vitro E3 ligase activity for some U-box
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