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23 Nov 2004: These reactionswere performed in KIN buffer {50 mM Mops ( pH 7.2),100 mM NaCl, 20 mM MgCl2, 0.5 mM ATP, and 2 mCiof [g-32P]ATP}. ... we solved the structureof the NH2-terminal domain from the closeAP180 homolog, CALM, at 2 Å resolution(19, 20) (crystals

1 May 2004: The best-characterizedsystem in this context is Spo0J fromB. subtilisand 8–10 copies ofcis-actingparSelements situated around 20% of theoriC region (86). ... 20. Cordell SC, L owe J. 2001. Crystal struc-ture of the bacterial cell division regulatorMinD.

4 May 2004: Only data extending to about 20/ resolution (within the first zero in the contrast transfer function) were used. ... Biochemistry 20, 2181-2191. Conti-Tronconi, B. M., McLane, K. E., Raftery, M.

30 Jan 2004: S2A), a neuron-specific guanosine triphos-phatase (GTPase)–activating protein involved inregulated exocytosis (19, 20). ... F) COS-7cells overexpressing rat amphiphysin1 wild-typeand mut1. Scale bar, 20 m.

16 Aug 2004: Mol. Biol. (2004) 341, 839–852. diverse roles18–20 that include anchoring the ring tothe cell membrane, invagination and constriction,septum formation and peptidoglycan synthesis toyield two separate daughter cells. ... After centrifugation, the

2 Feb 2004: Add reducing agents immediately before use. Minimal medium (per litre):1l M9 medium2ml 1M MgSO4 (2mM)20ml 20% glucose (0.4%)antibiotic(s)1ml vitamins 1000x (see below)10ml trace elements

2 Jun 2004: glass coverslips in 24-well plates were infected with trans-fected parasites and were processed for IF 20 –24 h post-transfection asdescribed (32). ... After 20 min on ice, themembrane fraction was isolated by centrifugation (100,000 rpm for 20min in a

15 Mar 2004: 20]. (c) Suppose that this year, instead of producing the units in-house, the firm. ... 700 20 1360 f c 28 1000 24 1160 g e,f 20 400 18 550.

1 Nov 2004: Cells were resuspended in sonication buffer (20 mM Tris [pH 8.0],the Vps25 subunit. ... 20 mM Tris [pH 7.4], 100 mM NaCl, and 2 mM DTT).

10 Aug 2004: PE. RS. ON. NE. L D. IVIS. ION. OR. GA. NIS. AT. ION. CH. AR. TA. NN. EX. B(. not. par. t of. the. Div. isio. n bu. t rep. ort t. o th. e D. irec. tor. ofP. erso. nnel. ). Occ. upat. iona. l Hea. lth. D. irec. tor o. fP. erso. nnel. P. eter. Dee. r.