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24 Sep 2004: substrate and product complexes.Cells were resuspended in buffer A (20 mM Tris pH 7.5 [4C], and100 mM NaCl) and disrupted with a French press. ... B.G.P. was supported by a fellowship from Secretarı́a de EstadoD [20 mM Tris (pH 7.5) (4C), 0.05 M

2 Feb 2004: Dense body. Synapticvesicles10–20 min at 19C. Temperature shift. 2004 Nature Publishing Group.

23 Nov 2004: www.sciencedirect.com Current Opinion in Cell Biology 2004, 16:379–391. G-proteins are required for membrane recruitment[18,20,30,31]. ... L. J Cell Biol 1964, 20:313-332. 2. Pearse BM: Coated vesicles from pig brain: purification andbiochemical

24 Sep 2004: Native protein was expressed in C41(DE3) cells. Cells were resuspended in buffer A (20 mM Tris, pH 8.0, 50 mMpotassium phosphate, pH 8.0, and 100 mM NaCl) and ... Fractions con-. taining Vps23 UEV were pooled and diluted with an equal volume ofbuffer C

16 Dec 2004: Signature of Staff Member. Date. Signature of Reviewer. Date. << /ASCII85EncodePages false /AllowTransparency false /AutoPositionEPSFiles true /AutoRotatePages /All /Binding /Left /CalGrayProfile (Dot Gain 20%) /CalRGBProfile (sRGB IEC61966-2.1)

10 Mar 2004: 959 24Comprehensive School 44,857 53,371 98,228 38,588 86 45,381 85 83,969 25Sixth Form College 16,500 20,495 36,995 14,418 87 17,706 ... West 846 6 219 6Overseas outside UK 2,774 20 414 12London 2,084 15 571 17South East 2,937 21 840 24.

3 Dec 2004: we solved the structureof the NH2-terminal domain from the closeAP180 homolog, CALM, at 2 Å resolution(19, 20) (crystals of AP180-N did not diffractwell). ... 20 November 2000; accepted 18 December 2000. Notch Inhibition of RASSignaling Through MAP

29 Dec 2004: the saturable component of bumetanide binding, Keq = k-l/k1 = 20 nM, was determined. ... 1 m. 0 IO 20 30 40. Time (min). 0 10 20 30 40 50 60 70 80.

30 Jan 2004: S2A), a neuron-specific guanosine triphos-phatase (GTPase)–activating protein involved inregulated exocytosis (19, 20). ... F) COS-7cells overexpressing rat amphiphysin1 wild-typeand mut1. Scale bar, 20 m.

17 Mar 2004: The negative surface potential, which isabout –30 mV for a membrane with 20% phos-phatidylserine [8], also attracts clusters of basicresidues on proteins. ... Dill, K.A., and Bromberg, S. (2003). Molecular Driving Forces.(Garland Science), Chapters