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21 Mar 2003: Dynamin GTPase assay and sedimentation assayThese assays have been described previously [20,27] and furtherdetails are given in the Supplementary material. ... Multimerised dynamin was then sedimented by ultracentrifuga-tion at 80,000 g for 20 min.

16 Jul 2003: and POPC/POPE/phos-phatidylinositol 3,4,5-trisphosphate (77:20:3) vesicles. ... a Increase in Kd relative to binding POPC/POPE/PtdIns(4,5)P2 (77:20:3) vesicles.b Not measurable.

20 Nov 2003: Crystals were equilibrated in 20% glycerol for cooling to 100K. The crystal asymmetric unit contains one molecule, and the crystals belong to spacegroup P3121, cell dimensions a = b = 49.6Å, c = ... 1mM TCEP. Sedimentation was at 11,000 rev/min, 20.0C,

21 Mar 2003: whereas glutamate release was essentially unaffected (96 9% ofmaximal; n = 3) in the presence of 20 µM cyclosporin A. ... Fol-lowing two washes, the synaptosomes were spun briefly a third timeand resuspended in lysis buffer (20 mM Hepes).

13 Jun 2003: 20) and Trusca et al. (21), using a MBP-SulAfusion or a protein A-SulA fusion, respectively, found SulA doesinhibit the GTPase activity of FtsZ. ... The reservoir was 7.5%polyethylene glycol (PEG) 20,0007.5% PEG monomethyl ether55080 mM sodium formate0.1

21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se,

9 May 2003: least two chemosensory neurons (AWA and AWC) has been measured to -20 mV, which. ... membranes. After 15-20 minutes of equilibration the cells were transferred into fresh.

16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023.

16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023.

15 Sep 2003: Thus with asingle image, there was too much noise for few, ifany, of the layer-lines to be distinguished convin-cingly beyond a resolution of about 20 AÊ. ... Common to all views are the 20 AÊwide vestibules on either side of the membrane,and a more

17 Mar 2003: presence of 20 ml 50% slurry of glutathione-following induction with 0.2 mM IPTG. ... Cell Biol. 20, 313–332.pathway in viral infection. EMBO J. 17, 4585–4593.

17 Mar 2003: controls, as well as release phase while the corresponding value in controlCPX I/II double mutant (2/2, 2/2; B) synapses (20,000-fold magnifi-. ... initial synaptic release probability is reduced in these concentrations of 0.5–20 mM, while the Mg21

4 Apr 2003: The signal retention improved by only 10% at low resolution (20 A), but by progressively greater amounts at higher resolutions, up to - 40% at 9 8. ... 20]; variable twist has been corrected by ap- plying an innovative real-space algorithm in a study of

5 Sep 2003: to-back interacting PB1 domains, with a freebuffer C (20 mM Tris, pH 7.4, 100 mM NaCl, and 5 mM DTT). ... C.D., Anderson, K.E., Butler, P.J., Lavenir, I., Perisic, O., Hawkins,GST/Par6b binding assays) and run on 20% Phast SDS gels.

17 Mar 2003: vesicle endocytosis. J. Neurosci. 20, 8667–8676. Ye, W., and Lafer, E.M. ... I. Developmental expres-sion and cellular localization of the F1-20 protein and mRNA.

6 Feb 2003: factor inthe same proteoliposomes was only. 15–20% of the control,raising the possibility that its transport may require stimula-tory factor(s) not needed by Prl. ... Only after the synthesis of an additional 20 resi-dues did the PrP–Prl intermediate

27 Oct 2003: being represented by 3 of the 20 ESTs for human golgin-(C) The oligomerization state of the His6-GRIP domain derived by 245 in the current GenBank database. ... The complex was concentrated ton X-100 in PBS, blocked with 20% (v/v) fetal calf serum/0.25%.

19 Jun 2003: holes, using a 300-kV field emission microscopeincorporating a top-entry liquid-helium-cooled stage10 (magnification, £36,800; defocusrange, 9,000–16,000 Å; dose, ,20 electrons Å22). ... 3H]diazofluorene. J. Biol. Chem. 273, 8659–8668 (1998). 20.

9 Jan 2003: 20, 2003 213–222http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. ... All GST fu-sion proteins were purified from bacterial extracts by incubation with glu-tathione-Sepharose beads, followed by extensive washing with 20 mMHepes, pH 7.4, 150 mM NaCl,

27 Nov 2003: EMBO J 2001,20:5813-5821. 49. Hinshaw JE: Dynamin and its role in membrane fission.Annu Rev Cell Dev Biol 2000, 16:483-519. ... EMBO J2001, 20:1819-1828. Structural/functional homology between the bacterial and eukaryotic cytoskeletons Amos, van den Ent

11 Sep 2003: Usually there are four or more stretches of about 20 hydrophobic amino acids per subunit or domain: the correct length for an a-helix to span the hydrophobic lipid chains. ... the contacting helices are inclined to one another by even greater angles:

24 Sep 2003: MR Z. EMMERICHIntroduction to political philosophy. Tu. 12. (Eight lectures, beginning 20 Jan.). ... PROF. T. MANSTEADEmotions. W. 12, Th. 10 (Four lectures, 13, 19, 20, 26.

12 Aug 2003: Criminological Research 5 7 12 — — —M.Phil. Criminology 8 12 20 — — —M.Phil. ... Archaeology and Anthropology 10 16 26 — — —Archaeology 20 30 50 18 15 33Biological Anthropology 12 26 38 5 4 9Social Anthropology 20 27 47 20 20 40.

23 Jul 2003: pen. sion. s0. 20. 40. 50. 20. 20. 40. 10. 20. ... 40. 10. 20. 40. 20. 20. 40. 20. 20. 419. Mis.

24 Sep 2003: 18, M. 20, W. 22DR D. J. TOLHURSTThe Motor System (Five lectures) M. ... GIBSONTestes Tu. 9 (20 Jan.); W. 4 (21 Jan.) (Two. Lectures)DR J.

30 Jun 2003: EMBO J. 20, 5383 – 5391. 12 Ma, J. and Lindquist, S.

10 Jan 2003: L. FOWDENSexual differentiation, M.10 (20 Jan.) (One Lecture) L.T. DR J. ... 12 (20 Feb.); Tu. 12 (25 Feb.) (Four Lectures) L.T.DR A.

6 May 2003: 20 CAMBRIDGE UNIVERSITY REPORTER [SPECIAL NO. 14. Consolidated Cashflow Statement for the year ended 30 September 2002. ... At 1 October 2001 20.47 3.74 0.20 2.75 27.16. TANGIBLE FIXED ASSETSUCLES.

23 Sep 2003: 9 (90 min). DR S. CASTELVECCHINineteenth-century music II (Twelve lectures,. beginning 15, 20 Jan.) Tu. ... 9.30 (90min). (Eight lectures and seminars, beginning 20 Jan.)Tu.11 (90 min).

24 Sep 2003: ELLARMacromolecules in the Cell. (Five lectures, beginning. 20 Oct.)DR M. TESTERMembranes: Molecular Superstructures. ... Eight lectures, 20 Apr.–6 May.)Please note the early start of this course.

28 Jan 2003: 1,700,649 — — (223,329) 1,218,822 1,218,822. 20 CAMBRIDGE UNIVERSITY REPORTER [SPECIAL NO. ... 476 — 21,476 (20,000) 1,476 — (23,377) 120,716 120,706. —

23 Jan 2003: PWF Club locationsChemical Engineering 16 10 220 72Economics 7 13 85 252Law (to December 2001) 5 5 20 66. ... Research Councils 61 (0.6%) 46 (0.5%) 37 (0.8%) 30 (0.7%)Other external (world-wide) 674 (6.3%) 489 (4.9%) 20 (0.4%) 24 (0.5%).

9 Feb 2003: for everyt 0, 1, 2,C. Assume that the initial number of wolves is 20 and the initialnumber of rabbits is 11, so thatx0 20, 11 I.a.

23 Sep 2003: MCKITTERICK. Medieval Research Seminar. Th. 5–7Weekly seminars beginning 20 May,History Faculty Boardroom. ... M.5 Fortnightly seminars beginning 20 October, DiracRoom, Fisher Building, St John’s College.

21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se,

28 Nov 2003: 20,000Sum transferred to General Reserve XXV from Estates £7,686,464 at 16%. ... 20,886Maintenance. 19,709 Transfers from other accounts: £Purchase of books, binding, printing, etc.

28 Jan 2003: Philosophy 661 32 — 693 20 — — 20 22 8Slavonic Studies 382 4 — 386 — — — — — —Spanish and Portuguese 526 4 — 530 16 — — 16 — —. 13,712 1,370 39 15,121 983 601 — 1,584 ... 035 Depreciation of buildings 15,457 13,426 Repairs

28 Apr 2003: Tuesday 20 May MU1. Practical examination, including improvisation plus submission of aportfolio of five compositions. ... English Gothic art and architecture, 1170–1350. 20. Visual encounters between Europe and the Ottoman Empire1.30–4.30 5.

24 Sep 2003: LENTONFactor Analysis. Th. 10–12 (Two two-hour lectures, 13,. 20 Nov.) Room B, Arts SchoolDR B. ... LANE AND DR P. WATSONTransition in post-communist societies. Tu. 2–5 (Four three-hour seminarsbeginning 20 Jan.) SPS Committee Room.

23 Jan 2003: 24–2613%. 22–2419%. 20–2234%. Student ages 2001–02 Staff Ages 2001–02. SPECIAL NO. ... and Caius College 6.7% 19%Homerton College 6.7% 14%Hughes Hall 4.0% 24%Jesus College 6.7% 20%King’s College 2.9% 11%Lucy Cavendish College

17 Dec 2003: Net funds at 31 July 20 173.0 170.1. 354 CAMBRIDGE UNIVERSITY REPORTER 17 December 2003. ... 20.9 7.4 63.9 92.2 43.4 Released to income and expenditure for depreciation.

24 Sep 2003: 2–5 TTR2MR J. OEPPEN. Advanced Multivariate Linear RegressionRegression Diagnositcs. Tu. 20 Jan. ... Part I) M. 20 Oct. 2–3.30. DR D. WEINBERGii) The Epistemological Foundations of Qualitative.

9 Apr 2003: DR N. MANSONRevision Seminar on Nietzsche, Tu. 20 May, 3–4 E.T. ... T. JUDD Access 2000: Further Use (2 sessions) May 19–20; July 9–10MR R.

23 Sep 2003: Theology. Tu. 2 (fortnightly seminarstarting 20 Jan.). The Qumran Text: Introduction and Readings.Th. ... fortnightly, beginning 21 Jan.). W. 2.30. The same continued. (fortnightly, beginning 20 Jan.).

28 Jan 2003: 815 — 20,131 20,946 33 — (2,047) 33,417 — 4,000 50 4,050 6,720 — — 2,347. ... W.H.O. 41,094 4W.T. GRANT FOUNDATION 224 1WALLENBERG EDUCATIONAL FOUNDATION 8,463 1WARNER-LAMBERT COMP 20,470 1.

24 Sep 2003: M. 2.15 (fortnightly, beginning 20 Oct.). A. Elementary LatnDR N. WRIGHT. ... Tu. 4.30 (fortnightlybeginning 20 Jan.). The same continued. Tu. 2.30 (fortnightly,beginning 27 Jan.).

24 Sep 2003: lectures)DR P. M. SEWELLSemantics of Programming Languages. Tu. Th. 10 (Twelve lectures, beginning 20 Jan.). ... RICHARDSData Structures and Algorithms. M. W. F. 11 (Eight. lectures, beginning 20 Oct.) Heycock Room,M.

8 Jul 2003: 5 C to 35 C, 85 % RH Max. -20 C to 50 C, 95 % RH Max.255 mm 54 mm 230 mm.

24 Nov 2003: Arts & Humanities 16.1% 10.3% 13.3% 8.5% 1.2% 4.5% 10.3%Humanities & Social Sciences 31.8% 34.8% 20.1% 14.0% 3.7% 8.7% ... E per £1,000 staff expenditure 58 11 69F per £1,000 research grant expenditure (RGE) 14 3 17G per £1,000 total expenditure,

24 Sep 2003: weeks 2, 4,. 6, 8: 23 Jan; 6/20 Feb; 5 Mar.) F. ... DIEMBERGERProject Management and Collaboration. (weeks 7–8:. 20/27 Nov.) Th. 5–6.30. 7.