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4 Jan 2006: fter. th. rom. bin cl. eava. ge o. f GST. ). 0.01. 0.1. 1. 10. 0 100 200 300 400. ln OD600. ... 1. 10. 0 100 200 300 400. ln OD600. pPLSC (E.coli LPAAT).

28 Nov 2006: Acetyl Transferase Assays. GST-MEK2 (0.5 g) (purified by usingstandard protocols for GST-tagged protein purification) wasincubated with varying amounts (0 –200 ng) of (His)6-YopJ-wt ... Stimulation of MEK and Erk phosphorylation by various

4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... J. Cell Biol. 155, 193–-200 (2001). 11. Huttner, W. B. & Schmidt, A.

12 Aug 2006: agent (200 ml in 700 ml water) was added and Pi release monitored. ... tios of these proteins were mixed (in buffer A plus 200 mM NaCl;.

17 Jan 2006: Bars, 200 nm. 4535Sorting Nexin-2 and CI-MPR retrieval. mammalian orthologue of the yeast retromer componentVps17p (Haft et al., 2000). ... J. Cell Biol. 155, 193-200. Gagescu, R., Gruenberg, J. and Smythe, E.

12 Nov 2006: Briefly, a 1 ml sample of each PX domain was loaded onto a Superdex 200 column (Amersham-Pharmacia) equilibrated and eluted with 20 mM Tris-HCl buffer, pH 7.4, containing

21 Nov 2006: 587 = 1,200,793. ... 29,200 Balance at beginning of year. 188,913Balance at end of year.

4 Jan 2006: 300 nm. 200 nm. 100 nm. b. c. d. and how the lipid and protein components can influence bilayertopology. ... J. Cell Biol. 155, 193–200 (2001). 67. Razzaq, A. et al.

28 Sep 2006: 50 nm. 100 nm. 200 nm. I. Supplementary Figure 2. The crystal structure of BDLP in GDP-associated and nucleotide-free conformation.

16 Jun 2006: 5) , andthe i r w i dth rema i ned fa i r l y constant (800 - 1 , 200 A).