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Madeline A. Lancaster Culturing feeder-dependent human iPS/ES cells…
https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Culturing-human-ES-and-iPS-cells.docx21 Mar 2014: 20 ng/ml bFGF (Add just before use). MEF Media (500 ml) – Store at 4C up to 2 weeks. ... 20 mg Dispase. 40 ml DMEM/F-12. Filtered using 0.22 um filter.
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