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14 Nov 2014: loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare) in 50 mM Tris pH 7.5, 150 mM NaCl, 1 mM βME. ... Crystals were cryo-protected by adding reservoir solution supplemented with 50-60 % (w/v) PEG 200, before flash freezing in liquid

19 Apr 2002: Insect cell extracts containing defined concentrations of Smc1 as the ligand (five dilutions, ranging from 20nM to 200 nM) were floated over the bound analytes, and association and dissociation kinetics were

17 Mar 2011: University of cambridge research magazine www.research-horizons.cam.ac.uk. issue 14. horizonsRESEARCH. In this issue. the world incambridge. plus news and views from across the University. 2 | Contents. research news 3–5. spotlight: 6–17 the

9 Sep 2008: form all of thebody’s approximately 200 different tissues.The regulatory interaction between stemcells and their local environment (knownas their ‘niche’) works somewhat like apoint-of-sale inventory system thatarranges for

4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... J. Cell Biol. 155, 193–-200 (2001). 11. Huttner, W. B. & Schmidt, A.

1 Feb 2008: Thinking Machine

1 Jul 2011: Von großem Vorteil ist dabei diedynamische Instabilität der ParM-Filamente:Die sich schnell wiederholende Abfolge vonFilamentbildung und -zerfall (ungefähr 200-mal schneller als bei Aktin) ermöglicht esParM, nach den Plasmiden zu

16 Aug 2008: tion plates mixing 200 200 nl of protein and crystallization solution. ... KOPS1 DNA. Crystallization solution was 200 mM citric acid/NaOH pH 3.5.

10 Oct 1997: Interestingly, although P2 (200 mM)efficiently blocks the Amph1– dynamin interaction, thebinding of Amph2 SH3 domain is much less wellinhibited (Figure 4B). ... GST-tagged SH3 domains were incubated as in A but in thepresence or absence of each of the

11 Oct 2007: After precipitation in 30% ammonium sulfate saturationand anion-exchange chromatography, the protein wasdialyzed against 50 mM Tris–HCl (pH 8.0), 200 mM KCl,1 mM EDTA, 10% (v/v) glycerol ... Drops were composed of100 nl of protein at 10.0 g/l (with 8

13 Aug 2019: The JEOL 2100F TEM was used with an accelerating voltage of 200 kV.

1 Nov 2019: the monitor, and then 'display drag factor'. Row a few strokes, and a number between 90 and 200. ... around 200. My resting heart rate is around 50 (best measured in bed right after waking up).

15 Mar 2004: Crash Cost (£). a none 32 200 26 500 b none 21 300 20 375 c none 30 200 30 200 d a 45 500 40 800 e a,b 26

5 May 2019: ility FtsL. 50 100 150 200 2500.0. 0.2. 0.4. 0.6. 0.8. ... 100. 200. 300. 400FtsB (22-103) FtsN (57-319) vs FtsQ. FtsN (57-319) vs FtsQ.

8 Aug 2011: Emmanuel 895,200.00 Lump sum. Girton 104,638.00 Annual. Gonville & Caius 167,574.78 Monthly. ... Magdalene 121,200.75 Monthly. Murray Edwards 90,976.50 Monthly. Newnham 54,441.00 Annual. Pembroke 114,876.00 Monthly.

18 Aug 2023: The court isbordered by a variety of shrubs. A magnificent Wisteria sinensis(pictured right), thought to bearound 200 years old, covers thesouth-facing wall. ... This enormous tree is thought to be morethan 200 years old, and is one of the largest

20 Jul 2007: mix - add 200 μl water, mix vigorously - centrifuge 2 min 10.000x g - take top phase off ( 500 μl), protein forms precipitated layer - add 300 μl methanol - centrifuged 1 min

4 May 2001: 200 column buffer. ... solution, andadditionally 200 mM NaI in the drop.

22 Nov 2021: 084.00£ 931.54£ 1,550.00£ 1,264.66£ JCR Fund 200.00£ 400.00£ 473.97£ Jesus Varsity 828.00£. ... £60.00Organicups £100.00Week of the Woman Speakers/Events £200.00Other sustainable period provision £500.00Galentine's Day £20.00General welfare

2 Apr 2007: buffer at 200 V for 1.5 hours.

12 Aug 2006: agent (200 ml in 700 ml water) was added and Pi release monitored. ... tios of these proteins were mixed (in buffer A plus 200 mM NaCl;.

24 Aug 2011: Bars, 200 nm. on August 24, 2011. jcb.rupress.orgD. ownloaded from. Published August 16, 2010. ... Bar, 200 nm. (E) NIH3T3 cells transfected with Cdc42-WT or -DN were incubated with CTxB before fractionation.

19 Jan 2017: vector and a 3’ primer that anneals 200 bp downstream of the stop codon of the open reading frame. ... DTT). During the buffer change, 1:200 of SuperTEV (for His-tagged proteins) or 3C protease (for GST-tagged proteins) were added for overnight

9 Nov 2018: 6. PUBLIC BENEFIT (continued) The College has some 200 part-time postgraduate students and its encouragement of part-time students allows individuals to benefit from a Cambridge education who might not ... 119. 0. 100. 200. 300. 400. 500. 600. 2012-13

16 Nov 2017: ltatio. n. Shared Facilities Hub. • Just under 5000sqm of Academic and A1- A4 floor space • 200 seats within cafeteria. •

30 Nov 2022: Dr Rishi Rajpopat First published online: 15 December 2022. Copyright Cambridge University Library. In Pānini We Trust:Discovering the Algorithm for Rule Conflict Resolution in the Astādhyāyī’. In Pāṇini We Trust Discovering the Algorithm

5 Dec 2012: Subsequently, Gb1His-g2 dimers were eluted with abuffer containing 20 mM tris-HCl (pH 8.0), 25 mM NaCl, 0.1% C12E10,200 mM imidazole, and 10 mM 2-ME. ... ME. Gb1His-g2(C68S) mutants were eluted with abuffer containing 20 mM tris-HCl (pH 8.0), 25 mM NaCl,

28 Feb 2024: Poland48 22 622 01 22 arfp@filantropia.org.pl. Fundaçao Oriente 35 12 135 85 200 administracao@foriente.pt.

27 May 2024: other cases considered but not approved. 2022-23 2021-22. Cases declined 200 168. ... Grave cause / other 302 172. Physical Health affecting the body 200 130 81 62 205.

23 Dec 2013: 1). An 200-amino-acid N-terminal do-main (FtsKN), which has four or five membrane-spanning seg-. ... C) Sizeexclusion chromatography-multiangle light scattering (SEC-MALS) analysis of TtFtsKN. Using a Superdex S-200 size exclusion column, samples were

20 Apr 2002: The cell pellet was resus-pended in 200 l of lysis buffer (1% Triton, 150 mM NaCl, 10 mM Tris,pH 7.4, 1 mM EDTA, 1 mM EGTA, 0.2 ... PLC2 was further purified on a heparin-Sepharose column (Am-ersham Biosciences) followed by gel filtration on a Superdex

9 May 2003: concentration of approximately 200 nM. Combined with the lack of putative voltage gated Na+ channels in the C.elegans. ... change, corresponding to an apparent free calcium concentration of approx. 200 nM.

25 Jan 2018: 52 5753. 6057. 99. 120 119. 109. 109110. 0. 100. 200.

7 Oct 2016: CAMBRIDGE COLLEGES’ FEDERATED. PENSION SCHEME. Actuarial Valuation. as at 31 March 2014. Scheme Funding Report. Prepared for the Management Committee. Acting as the Trustee of the. Cambridge Colleges’ Federated Pension Scheme. Robert Sweet

19 Mar 2013: Protein was then eluted by adding PrSc proteaseto the beads and further purified by Superdex 200 gel filtration.Protein Labeling—Proteins were labeled with Alexa dyes. ... J. Cell Biol. 155, 193–200. 22. Takei, K., Slepnev, V. I., Haucke, V., and De

17 Jan 2006: Bars, 200 nm. 4535Sorting Nexin-2 and CI-MPR retrieval. mammalian orthologue of the yeast retromer componentVps17p (Haft et al., 2000). ... J. Cell Biol. 155, 193-200. Gagescu, R., Gruenberg, J. and Smythe, E.

13 Apr 2016: 0. 100. 200. 300. 400. Vid. eo h. its. PlantSci 2014-5. ... 0. 50. 100. 150. 200. Vid. eo h. its. Biochemistry2015-6. (not part of project).

20 Jan 2023: 2002 Nigerian journalist Isioma Daniel incenses Muslims by writing about the Prophet Mohammed and Miss World, provoking riots which leave more than 200 dead.

12 Apr 2016: www.cam.ac.uk/annual-report. Reports and Financial Statements for the year. ended 31 July 2015. Contents. Annual Report of the Council for the academical year 2014–15 1. Annual Report of the General Board to the Council for the academical year

17 Jul 2024: 784Hospitality and entertainment 135 133 138 149 137 150Repairs and maintenance 397 499 472 478 415 421Study and Educational Expenditure 197 206 200 232 210 222Communications 52 42 40 77 52

16 Sep 2020: divest from the top listed7 200 oil, gas, and coal companies to the goal of divesting from all direct.

30 Aug 2001: S8 H4 S9 S10 S11 H5. 200 210 220 230 240 250ER.GYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYELP.DGQVITIG.NKTYNLMI.GDRTAEAIKMEIGSAE.APEESD.NMEIRGRDLLTGLPKTIEITGKETYRVAI.GERTAERVKIEIGNVF.PSKEND.ELETTVSGIDLSTGLPRKLTLKGG. ... 200 210 220 230 240. H6 H7 S12 S13

29 Dec 2017: anti-biotin antibody. F 127. catastrophe time. 0. 0.5. 1.0. time (seconds) 200 800. ... of the reaction was pipetted onto freshly glow-dischargedCu/Rh Quantifoil R2/2 200 mesh holey carbon grids (Quantifoil).

17 Feb 2024: The token system got positive reviews. ii. Sponsorship confirmed. More money confirmed for BOP (200 pounds).

13 Jun 2022: 150. 50. 250. mAU. 100. 200. 150. 50. 250. mAU. elution volume (ml)5 1510 20. ... 12.4 mlβ-amylase(200 kDa). 18.9 mlCytochrome c. (12.4 kDa). 17.4 mlCarbonic anhydrase.

31 Jan 2020: Superose 6 Increase 3.2/300 column (GE Healthcare), equilibrated with buffer T200 (50 mM Tris-HCl, 200 mM NaCl, pH 8.0). ... equili-brated with 20 mM Hepes, 200 mM NaCl at pH 7.5.

1 Mar 2024: CdpB2. SepF. SepFCdpB1. 200. Abso. rban. ce (a. u.). Abso. rban. ... For 1 transformation, 200 µl of the prepared cell suspension was used.

24 Oct 2011: University of Cambridge research

17 Jul 2024: The College has approximately 430 undergraduates and 200 graduates, a Fellowship of 67, and about 130 members of staff.

29 Aug 2021: 200 mM NaCl, 2 mM MgCl2,and 1 mM Tris(2-carboxyethyl)phosphine (TCEP). ... fast, direct-electron detector (Falcon 4,248 Hz), allowed us to acquire more than 200 micrographs per hour for 14 con-secutive days.