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Bb9e55.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Vallis_McMahon1999.pdf21 Mar 2003: Dynamin GTPase assay and sedimentation assayThese assays have been described previously [20,27] and furtherdetails are given in the Supplementary material. ... Multimerised dynamin was then sedimented by ultracentrifuga-tion at 80,000 g for 20 min. -
Contrasting Membrane Interaction Mechanisms of AP180N-terminal…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Stahelin_cho2003.pdf16 Jul 2003: and POPC/POPE/phos-phatidylinositol 3,4,5-trisphosphate (77:20:3) vesicles. ... a Increase in Kd relative to binding POPC/POPE/PtdIns(4,5)P2 (77:20:3) vesicles.b Not measurable. -
Membrane curvature sensing and induction: The function of BAR domains …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/BARdomains/BARsupp.pdf20 Nov 2003: Crystals were equilibrated in 20% glycerol for cooling to 100K. The crystal asymmetric unit contains one molecule, and the crystals belong to spacegroup P3121, cell dimensions a = b = 49.6Å, c = ... 1mM TCEP. Sedimentation was at 11,000 rev/min, 20.0C, -
740 Research Paper Calcium triggers calcineurin-dependent synaptic…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Marks_McMahon1998.pdf21 Mar 2003: whereas glutamate release was essentially unaffected (96 9% ofmaximal; n = 3) in the presence of 20 µM cyclosporin A. ... Fol-lowing two washes, the synaptosomes were spun briefly a third timeand resuspended in lysis buffer (20 mM Hepes). -
Crystal structure of the SOS cell division inhibitorSulA and ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SulA%20PNAS%202003.pdf13 Jun 2003: 20) and Trusca et al. (21), using a MBP-SulAfusion or a protein A-SulA fusion, respectively, found SulA doesinhibit the GTPase activity of FtsZ. ... The reservoir was 7.5%polyethylene glycol (PEG) 20,0007.5% PEG monomethyl ether55080 mM sodium formate0.1 -
554 Research Paper Inhibition of receptor-mediated endocytosis by the …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se, -
3747_0_supp_7_1052456676
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/christian.pdf9 May 2003: least two chemosensory neurons (AWA and AWC) has been measured to -20 mV, which. ... membranes. After 15-20 minutes of equilibration the cells were transferred into fresh. -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/sedequil.pdf16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/sedequil.pdf16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. -
Nicotinic Acetylcholine Receptor at 4.6 Å Resolution: Transverse…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jmb99.pdf15 Sep 2003: Thus with asingle image, there was too much noise for few, ifany, of the layer-lines to be distinguished convin-cingly beyond a resolution of about 20 AÊ. ... Common to all views are the 20 AÊwide vestibules on either side of the membrane,and a more
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