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  2. Madeline A. Lancaster Culturing feeder-free human ES cells Modified…

    https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Culturing-feeder-free-human-ES-cells.docx
    21 Mar 2014: 2. Prepare new Matrigel plates as above or warm pre-prepared Matrigel coated dishes in the incubator. ... Add additional media needed. c. Generally minimize pipetting! 8. Transfer 0.5 ml cells per new well and place back in the incubator, being careful
  3. Model Building and Refinement Practical Introduction In this…

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/mbar.docx
    27 Apr 2016: We will make use of the new NCS tools in the latest version of refmac5. ... The two new fields that appear can be left at their default values.
  4. Carter lab dynactin prep from frozen porcine brains, using ...

    https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/2020_Carter_dynactin_brain_prep.docx
    28 Feb 2020: Get one new zip lock bag per brain, and open them so that they are easy to handle with dirty hands later on. ... Put brain in new zip lock bag and wash in PBS.
  5. Madeline A. Lancaster Culturing feeder-dependent human iPS/ES cells…

    https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Culturing-human-ES-and-iPS-cells.docx
    21 Mar 2014: Add 1 ml hES media to well. 10. Resuspend hiPSC pellet in 1 ml hES media per new well of 6-well plate and triturate gently with P1000 tip to get ... 7. Wash the old well with another 1 ml hES media and transfer to new well.
  6. Word macros that will translate a highlighted area of ...

    https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/computer/word_macros/TranslateIncNucleotide2.docx
    4 Feb 2014: Selection.Font.Name = "Courier New". Selection.Font.Size = 8. Selection.TypeText (output).
  7. Model Building and Refinement Practical Introduction In this…

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ed-lowe-mb.docx
    27 Apr 2016: We will make use of the new NCS tools in the latest version of refmac5. ... The two new fields that appear can be left at their default values.
  8. 31/10/2018 Carter lab TIRF microscopy Single Molecule Assay 5x ...

    https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_TIRF_protocol.docx
    28 Jan 2020: After 3 hours or so, make a new dilution. Consider also spinning the 250 µL stock if background is still high.
  9. Clockology Survey Thank you for your interest in participating ...

    https://www2.mrc-lmb.cam.ac.uk/groups/oneill/wp-content/uploads/sites/24/Clockology-Survey-FINAL_14_07_2020_COMBINED.docx
    14 Jul 2020: The phase before the stimulus is plotted against the difference between the new phase and the old phase. ... Phase transition curve: a curve describing the relationship between the circadian phase at which a stimulus is applied and the resultant new

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