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  2. A Coot for Cryo-EM: 0.9 (pre-release) ● ~1500 revisions ...

    https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/CCP4-wg2-whats-new-in-Coot-2019.pdf
    28 May 2019: A Coot for Cryo-EM: 0.9 (pre-release). 1500 revisions ahead of the master branch This new branch is actively developed Considerations for the CootVR interface. ... Ignore it. – new Coot on Mac OS X and Windows doesn't matter.
  3. Model-Building with Modern Coot Paul Emsley Scripps, Feb 2019 ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Coot-Scripps-Feb-2019-EOS.pdf
    21 Feb 2019: Less stable More interesting Not always clear how to use the new functions. ... or that the new functions are there). A New Branch. New multi-threaded sections: Refinement. –
  4. Activation of GCN2 by the ribosomal P-stalk

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/PNAS_2019.pdf
    7 Mar 2019: New insight into a possible functional link be-tween GCN2 and ribosomes came from a recent analysis of micelacking both a specific neuronal tRNA (tRNAArgUCU) and theputative ribosome recycling factor GTPBP2
  5. Current Developments in Coot for Macromolecular Model Building…

    https://www2.mrc-lmb.cam.ac.uk/coot/protein-science/Casanal_Lohkamp_Emsley_11112019-pe-2.pdf
    11 Nov 2019: Faster refinement goes hand in hand with the addition of new restraint types. ... library. The new mode of Acedrg allows the generation of bespoke chemical links,.
  6. The structure of bactofilin filaments reveals their mode of membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf
    26 Aug 2019: from genomic DNA and cloned into vector pET15b using Gibson assembly (New England Biolabs). ... plasmid pHis17 using Gibson assembly (New England Biolabs) with a stop codon before the C-terminal tag on the plasmid.
  7. Mechanism of ribosome stalling during translation of a poly(A) tail

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/NSMB_2019.pdf
    25 Nov 2019: R., Mohr, S. C. & Smith, T. F. Genomic detection of new yeast pre-mRNA 3’-end-processing signals.
  8. Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Szewczak-Harris_et_al-2019-FEBS_Letters.pdf
    22 Jul 2019: form a new class of alternating, copolymeric filaments,. which can be assembled in vitro either on lipid mem-. ... Hagen WJ, Lindahl E and Scheres SH (2018) New. tools for automated high-resolution cryo-EM structure.
  9. 15591370691369 1..30

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf
    29 May 2019: the presence of 100 mg/ml cycloheximide to suppress new protein synthesis. ... tion attenuation during acute ER stress, new GFP-PrP synthesis during the time course is relatively.
  10. Recognition and Degradation of Mislocalized Proteins in Health and…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CSHL_Persp_2019.pdf
    8 Mar 2019: 3). First, the load of new proteinsentering stressed mitochondria is reduced at theexpense of generating mislocalized proteins thatmust be degraded by cytosolic proteasomes(Wright et al.
  11. Biallelic Variants in ASNA1, Encoding a Cytosolic Targeting Factor of …

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CircGen_2019.pdf
    1 Dec 2019: TA proteins in early embryonic development.11–16 This study offers the first evidence for its role in human disease, and provides new insight into the molecular mechanisms in DCM.

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