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6. Image refinement Sjors Scheres EM course 2017 Agenda ...
https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-06-refinement/?wpdmdl=18760&refresh=6691470fed2b517207969434 Oct 2017: calculate new 2D average as probability weighted averages. Reference-free 2D class averaging. ... The bad news • The experimental data alone is not enough to determine a unique solu7on! • -
Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf29 May 2017: Added into a new N-linked tree: – paucimannose. 23/40. Xyl-β1,2. Xyl - β1,2 – Man – using XYP (beta D xylosepyranose). – ... both GUI interface and built into the auto-builder. New Interface. -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: 3), requiring RsaA to adoptslightly different conformations. Furthermore, gaps in the latticewill be needed for insertion of new RsaA molecules on growingcells. ... The new culture was grown at 30 C for2–3 days. The culture was imaged using a -
Tomography_2017
https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-09-tomography/?wpdmdl=18762&refresh=669147102342d17207969446 Oct 2017: Missing information due to discrete sampling. Data collection. Set new tilt angle. ... aligned toreference subtomograms. (aligned). averaged subtomograms(new reference). use new reference for alignmentiterate until reference is stable. -
ligand-fitting-tutorial.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ligand-fitting-tutorial.pdf24 Oct 2017: The centre of the screen is a bit crowded now. Undisplay those new ligands. ... When we are happy with our fit, we can merge this new ligand into the mainprotein ligand as we did before. -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: dejongeii DSM 12251 (PdBtubAB), a stop codon (TAA)was introduced before the C-terminal hexahistidine tag in the BtubAB bacterialT7 expression plasmid used (7) by Q5 mutagenesis (New England Biolabs). ... E5954 | -
Fei Long MRC Laboratory of Molecular Biology, Cambridge, U.K. ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Ligand_meeting_Sept_29_2017.pdf29 Sep 2017: new_atom_id _chem_mod_atom.new_type_symbol _chem_mod_atom.new_type_energy _chem_mod_atom.new_charge TMPmod1 delete H2. ... chem_mod_bond.new_type _chem_mod_bond.new_value_dist _chem_mod_bond.new_value_dist_esd TMPmod1 delete C1 H2 single. -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: These constructs are in a vector (provided with the PURE system kit from New England Biolabs) that contains a T7 promoter and transcriptional terminator. ... The resin was washed four times with 1 ml PSB2 and transferred to a new tube after the fourth -
A Polymerization-Associated Structural Switch in FtsZ That Enables…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf3 May 2017: the divisome around the division site (17, 18).These findings have resurrected an old model of bacterial cell division: the templatemodel, in which the closing septum constricts by new cell wall ... because the energetic cost of losing subunits from ends -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: using the Gibson Assembly kit (New England Biolabs).
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