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First complete 3D genome structure from individual mammalian cells -…
https://www2.mrc-lmb.cam.ac.uk/first-complete-3d-genome-structure-individual-mammalian-cells/13 Mar 2017: However, using a new chromosome conformation capture technique, Tim Stevens from the LMB’s Cell Biology Division, in collaboration with Ernest Laue’s group in the University of Cambridge Department of
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Using cryo-EM to solve the structure of a protein complex critical…
https://www2.mrc-lmb.cam.ac.uk/using-cryo-em-solve-structure-protein-complex-critical-gene-expression/7 Nov 2017: A Log-in or subscription may be required to view this article from the host site: https://www.drugtargetreview.com/news/27088/cryo-electron-microscopy-cpf/].
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Wojciech Galej receives Early Career Research Award from the…
https://www2.mrc-lmb.cam.ac.uk/wojciech-galej-receives-early-career-research-award-biochemical-society/11 Apr 2017: Wojciech Galej has been awarded the 2018 Early Career Research Award for Molecular Structure and Function by the Biochemical Society. Wojciech has made
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LMB postdoc, Tobias Wauer, wins the 2017 EMPRIS Award for Research in …
https://www2.mrc-lmb.cam.ac.uk/lmb-postdoc-tobias-wauer-wins-2017-empris-award-research-brain-diseases/13 Dec 2017: LMB postdoctoral researcher, Tobias Wauer, has won the 2017 EMPRIS Award for Research in Brain Diseases for his work on the molecular causes of
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Alison Woollard appointed Academic Champion for Public Engagement…
https://www2.mrc-lmb.cam.ac.uk/alison-woollard-appointed-academic-champion-public-engagement-research-oxford/15 Feb 2017: Alison Woollard, LMB 1996-2000, Postdoctoral Fellow, Cell Biology Professor Alison Woollard, Department of Biochemistry and Fellow of Hertford College,
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Student artist meets LMB scientist who discovered inspirational…
https://www2.mrc-lmb.cam.ac.uk/student-artist-meets-lmb-scientist-discovered-inspirational-toxins-structure/9 Mar 2017: Vivian, student artist who created a painting based on lysenin during Protein Data Bank in Europe’s art-science collaborative project, met with LMB’s
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Visualising the genome: Cambridge scientists create first 3D…
https://www2.mrc-lmb.cam.ac.uk/visualising-genome-cambridge-scientists-create-first-3d-structures-active-dna/16 Mar 2017: Tim Stevens, in collaboration with scientists from the University of Cambridge, has determined the first 3D structures of intact mammalian genomes from
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Richard Henderson and Nigel Unwin awarded Gjønnes Medal in Electron…
https://www2.mrc-lmb.cam.ac.uk/richard-henderson-nigel-unwin-awarded-gjonnes-medal-electron-crystallography/29 Aug 2017: The International Union of Crystallography (IUCr) Gjønnes Medal in Electron Crystallography has been awarded to Richard Henderson, Group Leader in the
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Rebecca Taylor to talk at Cambridge Science Festival: Thursday 23rd…
https://www2.mrc-lmb.cam.ac.uk/rebecca-taylor-talk-cambridge-science-festival-thursday-23rd-march-6-00pm/28 Feb 2017: Rebecca Taylor will be giving a talk at the UTC Cambridge as part of the Cambridge Science Festival. In her talk entitled, 'Finding the fountain of youth:
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Network control principles predict neuron function in the C. elegans…
https://www2.mrc-lmb.cam.ac.uk/network-control-principles-predict-neuron-function-c-elegans-connectome/19 Oct 2017: This work provides new and powerful theoretical tools to allow us to interpret these brain maps and understand the how the nervous system works at the most fundamental, cellular level.
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Hundreds of children and adults enjoy the LMB’s ‘See Your Cells’…
https://www2.mrc-lmb.cam.ac.uk/hundreds-children-adults-enjoy-lmbs-see-cells-activity-cambridge-science-festival/23 Mar 2017: The first weekend of the 2017 Cambridge Science Festival saw over 3000 visitors attend the 'Hands-on at the Guildhall' event, where the LMB’s 'See Your
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Finding the founding fathers of molecular biology – a blog post by…
https://www2.mrc-lmb.cam.ac.uk/finding-founding-fathers-molecular-biology-blog-post-lmb-phd-student-lisa-strittmatter/6 Dec 2017: Lisa Strittmatter, PhD student in the LMB’s Structural Studies Division, explores a recent talk given at the LMB by Professor Sir John Meurig Thomas, and
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G-protein coupled receptors as drug targets: Heptares Therapeutics…
https://www2.mrc-lmb.cam.ac.uk/g-protein-coupled-receptors-drug-targets-heptares-therapeutics-celebrates-tenth-anniversary/6 Jul 2017: Chris Tate – co-founder of Heptares Therapeutics, a company established to commercialise research from the LMB – discusses his work on G-protein coupled
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Scientists establish a comprehensive protein interactions map of the…
https://www2.mrc-lmb.cam.ac.uk/scientists-establish-comprehensive-protein-interactions-map-replication-machinery-chronic-virus/21 Dec 2017: Chronic viral infections are amongst the biggest threats to human health worldwide. Leo James’ group from the LMB’s PNAC Division, in collaboration with
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LMB scientist, Glenn Masson, spends a week shadowing MP for…
https://www2.mrc-lmb.cam.ac.uk/lmb-scientist-glenn-mason-spends-week-shadowing-mp-cambridge-daniel-zeichner-royal-societys-pairing-scheme/13 Dec 2017: Glenn Masson has spent a week shadowing Daniel Zeichner, MP for Cambridge, through the Royal Society’s Pairing Scheme, an initiative which pairs
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Pymol guide
https://www2.mrc-lmb.cam.ac.uk/groups/nagai/download/pymolguide.pdf19 Dec 2017: So if you click on (sele) you can toggle and untoggle your new selection. ... So if you want to make a new selection, make sure you deselect everything first! -
6. Image refinement Sjors Scheres EM course 2017 Agenda ...
https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-06-refinement/?wpdmdl=18760&refresh=668d521415d8217205376204 Oct 2017: calculate new 2D average as probability weighted averages. Reference-free 2D class averaging. ... The bad news • The experimental data alone is not enough to determine a unique solu7on! • -
Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf29 May 2017: Added into a new N-linked tree: – paucimannose. 23/40. Xyl-β1,2. Xyl - β1,2 – Man – using XYP (beta D xylosepyranose). – ... both GUI interface and built into the auto-builder. New Interface. -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: 3), requiring RsaA to adoptslightly different conformations. Furthermore, gaps in the latticewill be needed for insertion of new RsaA molecules on growingcells. ... The new culture was grown at 30 C for2–3 days. The culture was imaged using a -
Tomography_2017
https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-09-tomography/?wpdmdl=18762&refresh=668d52144296817205376206 Oct 2017: Missing information due to discrete sampling. Data collection. Set new tilt angle. ... aligned toreference subtomograms. (aligned). averaged subtomograms(new reference). use new reference for alignmentiterate until reference is stable. -
ligand-fitting-tutorial.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ligand-fitting-tutorial.pdf24 Oct 2017: The centre of the screen is a bit crowded now. Undisplay those new ligands. ... When we are happy with our fit, we can merge this new ligand into the mainprotein ligand as we did before. -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: dejongeii DSM 12251 (PdBtubAB), a stop codon (TAA)was introduced before the C-terminal hexahistidine tag in the BtubAB bacterialT7 expression plasmid used (7) by Q5 mutagenesis (New England Biolabs). ... E5954 | -
Fei Long MRC Laboratory of Molecular Biology, Cambridge, U.K. ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Ligand_meeting_Sept_29_2017.pdf29 Sep 2017: new_atom_id _chem_mod_atom.new_type_symbol _chem_mod_atom.new_type_energy _chem_mod_atom.new_charge TMPmod1 delete H2. ... chem_mod_bond.new_type _chem_mod_bond.new_value_dist _chem_mod_bond.new_value_dist_esd TMPmod1 delete C1 H2 single. -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: These constructs are in a vector (provided with the PURE system kit from New England Biolabs) that contains a T7 promoter and transcriptional terminator. ... The resin was washed four times with 1 ml PSB2 and transferred to a new tube after the fourth -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: using the Gibson Assembly kit (New England Biolabs). -
A Polymerization-Associated Structural Switch in FtsZ That Enables…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf3 May 2017: the divisome around the division site (17, 18).These findings have resurrected an old model of bacterial cell division: the templatemodel, in which the closing septum constricts by new cell wall ... because the energetic cost of losing subunits from ends -
REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf19 Jan 2017: PURE system translation reactions. Initial experiments used the PURE translation system from New England Biolabs as per the manufacturer’s instructions. -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf22 Sep 2017: authors. Thesedevelopments along with advances in structure determination techniques (mainly the use ofsynchrotrons, area detectors and crystal freezing) led to an enormous increase in new proteinstructures: in 1990, about 500 structures
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