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28 Apr 2010: There are still questions about how the structure recognizes the very slight curvature of the polar membrane, but the new structural information greatly advances the discussion.

2 Aug 2010: The title. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. ... This can becrucial in the design of new generations of Vps34inhibitors with improved specificity, solubility,and cellular

21 Oct 2010: The identificationof quadruple as well as double filaments posed a new question,however: Which filament is present in cells? ... Both formed filaments similar to those of native TubZ. We then reconstructed the new filaments in three dimensionsto reveal

2 Aug 2010: We devised a new expression and purification strategy by introducing a TEV protease. ... All propeller-shaped inhibitors create a new specificity pocket not present in the apo form of the enzyme.

17 Feb 2010: References. Adams PD, Grosse-Kunstleve RW, Hung LW, Ioerger TR, McCoy AJ,Moriarty NW, Read RJ, Sacchettini JC, Sauter NK, Terwilliger TC(2002) PHENIX: building new software for automated crystal-lographic ... Mol Microbiol 51:1321–1332. Gerdes K (2009)

14 Sep 2010: Filaments stabilized in this way continue to polymerize with the addition of new ParM subunits at both ParR–parC-capped ends, although the actual rate of elongation of an individual

20 May 2010: Corresponding authors. LW Hamoen, The Centre for Bacterial CellBiology, Medical Science New Building, Medical School, NewcastleUniversity, Richardson Road, Newcastle upon Tyne NE2 4AX, UK.Tel.: þ44 191 208 3240; Fax: þ44 ... These were then removed

2 Aug 2010: This conformational changelifts the phenylalanine residue of the DFG motif to disruptits interaction with helix αC, thereby creating a new hydro-phobic pocket deep behind the ATP-binding site that has ... For these inhibitors, a new pocket is formed

20 Jul 2010: Journal of Molecular Biology, doi:10.1016/j.jmb.2010.06.030

30 Apr 2010: Stable for at least 2 years.4. T7 or SP6 polymerase (New England Biolabs). ... chromatography resin, wash extensively with bufferscontaining the desired detergent, and elute in a buffer contain-ing this new detergent.