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26 Sep 2011: Myc-EpsinR with endogenous TGN46 and AP1 in COS cells. In the image to the left there are 2 cells transfected with Myc-epsinR and 3 cells not transfected. ... In the left cells endogenous AP1 is finely punctate and co-localises with the more tubular

26 Sep 2011: For a detailed study of endogenous epsinR and overexpression of wild-type and mutants in cells see immunocytochemistry.

14 Jul 2011: endogenous ubiqui-tin was more than 90% competed out, resulting in few or no untagged ubiquiti-nated products. ... The absence of endogenous charged tRNAs and haemoglobinincreased photocrosslinker incorporation and photolysis, respectively.

11 Nov 2011: We also examined whether archaeal TRC40 could func-tionally replace the endogenous mammalian TRC40 atphysiological concentrations. ... absence of endogenous mam-malian TRC40 using a phenyl- and Diethylaminoethyl (DEAE)-Sepharose-depleted RRL (PD-RRL)

15 Nov 2011: To evaluatePtdIns(4,5)P2 content in the plasma membrane and to competefor PtdIns(4,5)P2 binding with endogenous PtdIns(4,5)P2-bindingproteins, we expressed PLCδ1PH (PH domain of ... Lowering the concentrations of PtdIns(4,5)P2available for interactions

24 Aug 2011: By Western blot analysis both endogenous and transfected Flot1 and GRAF1 were concentrated in Fraction 2.8 (Fig. ... 4 G, arrowheads). Endogenous dynamin labeled a significant population of struc-tures within Fraction 2.8 (Fig.

21 Feb 2011: Previous characterization ofBAR domain proteins has generally involved overproduc-tion, raising the need for in vivo studies characterizing theconsequences of BAR domain mutations in genes expressedat their endogenous level. ... these alterations affect

11 Nov 2011: This indicated that BirA has no detectable targetsendogenous to mammalian cells, and that the BioTag is notmodified by endogenous enzymes. ... Note that lowlevels of endogenous PrP are visualized at 35kDa in the SS-PrP samples probed with-PrP.

15 Sep 2011: To overcome problems caused by protein overexpression, our laboratory has used Zinc Finger Nuclease (ZFN) technology to express RFP- and GFP-tagged versions of clathrin and dynamin, respectively, preserving their endogenous

11 Nov 2011: stable cell lines expressing either wild-type PrP or osteopontin (Opn)-PrP at levels comparable to endogenous PrP in brain (Kang et al., 2006).