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10 Oct 1997: We interpret these results tomean that either Amph1 or Amph2 overexpressionalone leads to sequestration of endogenous dynamininto inactive complexes, but when the two proteins areexpressed together, forming a heterodimer, endocyto-sis

16 Feb 2010: 2007). To maximize the severity of any phenotype, PrP+/ mice were used because endogenous PrP was observed in earlier studies to exacerbate CtmPrP-mediated neurodegeneration (Stewart et al., 2005).

16 Jul 2008: SDS-PAGE, andimmunoblotted using an antibody (3F4) that specifically detects transfected but not endogenous PrP. ... C) Biosynthesisand maturation of endogenous PrP in A4 and L-cells were assessedby pulse-chase analysis followed by immunoprecipitation

8 Nov 2004: Endogenous NF-kB (indicated by an aster-isk) serves as a loading control. ... Immunoblots were probed with antibodiesagainst PrP, GFP, and an endogenous protein, the beta subunit ofSec61.

30 Apr 2010: Itdoes, however, stimulate the translation of some proteins(presumably because the endogenous levels of some tRNAsin the RRL are limiting), and it is possible that some RRLpreparations contain relatively low amounts ... 5. Endogenous RRL usually contains

18 Oct 2008: a high concentration of an endogenous PDZ-binding. Figure 5: The C-termini of GluR2 and of DAT fused to thesingle transmembrane protein Tac are sufficient for BARdomain-dependent coclustering with ... This. juxtanuclear clustering strongly resembles the

18 Feb 2016: Ribosome profiling in the presence and absence of Dom34 has shown that an endogenous substrate for this pathway is Hac1, a cytosolically spliced mRNA whose incorrect ligation results in an mRNA ... The key open questions include the following: (i) What

21 Feb 2011: Previous characterization ofBAR domain proteins has generally involved overproduc-tion, raising the need for in vivo studies characterizing theconsequences of BAR domain mutations in genes expressedat their endogenous level. ... these alterations affect

2 Aug 2010: 26]. The p101 subunitprotects cells from apoptosis when overexpressed, and thismay explain why it is a common site for insertion in T-celllymphomas induced by the MoFe2-MuLV retrovirus [27].The

10 Jan 2013: siRNA of endogenous protein led to decreased cell migration, increasedphagocytosis and reduced podosome density and dynamics. ... Both endogenous and overexpressed Bin2-EGFP. localized to ring-like structures around the actin core of.

9 Sep 1998: We believe this effectis due to sequestration of endogenous dynamin away fromclathrin-coated pits.

11 Aug 2015: By contrast, endogenous SRP in a homologous mammalian system hasa remarkably subtle effect on translation elongation (Wolin and Walter, 1989). ... Here we demonstrate that endogenous SRP is efficiently recruited to mammalian ribosomescontaining a

26 Aug 2021: Othersubunits of the 19S base were copurified atlower levels, consistent with partial assemblyof PSMC5 with endogenous base subunits pres-ent inthe lysate (table S5). ... Boosting HERC1 levelsusing CRISPR activation (31) of the endoge-nous HERC1 promoter

17 Jan 2012: Instead, both endogenous and overexpressed GRAF1 were found enriched at small, round, vincu-lin-positive structures in a subset of cells (see Figure S1, C and D). ... Cells were acid-stripped and fixed and were then labeled for endogenous paxillin.

24 Aug 2011: By Western blot analysis both endogenous and transfected Flot1 and GRAF1 were concentrated in Fraction 2.8 (Fig. ... 4 G, arrowheads). Endogenous dynamin labeled a significant population of struc-tures within Fraction 2.8 (Fig.

15 Sep 2011: To overcome problems caused by protein overexpression, our laboratory has used Zinc Finger Nuclease (ZFN) technology to express RFP- and GFP-tagged versions of clathrin and dynamin, respectively, preserving their endogenous

31 Dec 2014: elifesciences.org. Szwedziak et al. eLife 2014;3:e04601. DOI: 10.7554/eLife.04601 1 of 22. Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell divisionPiotr Szwedziak†, Qing Wang†, Tanmay A M Bharat, Matthew Tsim, Jan

5 Aug 2013: Second, the system is typicallyorthogonal to endogenous pathways and utilizes a single definedligase. ... Furthermore, the absolute dependence on phospho-Vpu illustrates that endogenous ligases in this system do notperceive mCD4 as a misfolded protein

14 Jun 2007: Many plasma-membrane proteins are in a constant flux throughout the internal trafficking pathways of the cell. Some receptors, such as that for transferrin, are continuously internalized into recycling endosomes and returned to the cell surface.

5 Jan 2016: ribosomes used for the cryo-tomography structure (16), thepresence or absence of heterogeneous endogenous nascentpolypeptides within Sec61 could not be determined conclusively.Thus, the conclusion that the Sec61 complex is constitutivelyopen