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REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf19 Jan 2017: endogenous proteins with recombinant variantsrevealed the functional roles for all of the factorsand interactions. ... C) Immunoblots (IB) for triage components of control or BAG6 complex-depleted RRL replenished with recombinant BAG6 sub-complexes used -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: Catalytically inactive UBE2O(C1040S) alsorecovered endogenous and exogenous RPL24(but no detectable ubiquitinated forms), whereasUBE2O(DCR2) interacted poorly with RPL24 (fig.S10D). ... The heterogeneity of ubiquitin bands in the samples with cytosol is
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