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4 Feb 2011: Recent and Recurrent Selective Sweeps of the Antiviral RNAiGene Argonaute-2 in Three Species of Drosophila. Darren J. Obbard,,1,2 Francis M. Jiggins,3 Nicholas J. Bradshaw,4 and Tom J. Little1,2. 1Institute of Evolutionary Biology, University of

11 May 2011: RNA was extractedand reverse-transcribed (see above), and qRT-PCR was used tomeasure the viral titer relative to an endogenous control(RpL32) using the delta delta CT method.

30 Oct 2009: Quantifying Adaptive Evolution in the DrosophilaImmune SystemDarren J. Obbard1, John J. Welch1, Kang-Wook Kim1,2, Francis M. Jiggins3. 1 Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom, 2 Department of Animal

16 Nov 2012: As an endogenous control weamplified Actin5c using the primers qActin5c_for2 (59GAGCGC-GGTTACTCTTTCAC 39) and qActin5c_rev2 (59 AAGCCTC-CATTCCCAAGAAC 39).We performed three technical repli-cates of each PCR and

2 Jul 2015: For carcasses and testes, the amount of the Wolba-chia gene atpD (atpDQALL_F: 5’-CCTTATCTTAAAGGAGGAAA-3’; atpDQALL_R: 5’-AATCCTTTATGAGCTTTTGC-3’) relative to the endogenous control gene actin 5C (Forwardprimer:

10 Aug 2012: UNCORRECTED PROOF Date: 12:15 Thursday 21 June 2012File: Rhabdoviruses 3P. 8The Sigma Viruses of DrosophilaBen Longdon, Lena Wilfert and Francis M. Jiggins. AbstractThe sigma virus of Drosophila melanogaster (DMelSV) was discovered accidentally over

24 Jan 2016: RESEARCH ARTICLE. A Polymorphism in the Processing BodyComponent Ge-1 Controls Resistance toa Naturally Occurring Rhabdovirus inDrosophilaChuan Cao, Michael M. Magwire¤a, Florian Bayer¤b, Francis M. Jiggins. Department of Genetics, University of

19 Sep 2014: using the GoScript Reverse Transcription System (Promega) with. random primers. Host gene expression and the Wolbachia densitywere measured relative to the endogenous control gene actin 5C(Table S3) using the SensiFAST

16 Jan 2014: Wolbachia Variants Induce Differential Protection toViruses in Drosophila melanogaster: A Phenotypic andPhylogenomic AnalysisEwa Chrostek1, Marta S. P. Marialva1, Sara S. Esteves1, Lucy A. Weinert2, Julien Martinez3,. Francis M. Jiggins3, Luis