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ppat.1002260 1..9
www.jiggins.gen.cam.ac.uk/pdfs/longdon2011c.pdf26 Sep 2011: The copy-number of viral genomic RNA was expressed. relative to the endogenous control housekeeping gene RpL32 (Rp49).We designed different RpL32 primers specific for each species. ... technical replicates) were carried out per sample with both the. -
15566153538820 1..21
www.jiggins.gen.cam.ac.uk/pdfs/duxbury2019.pdf30 Apr 2019: endogenous control primers. Each qRT-PCR plate contained three standard samples, and all experi-. ... of the virus and the endogenous control. Viral load calculated without using the endogenous control. -
pone.0006838 1..11
www.jiggins.gen.cam.ac.uk/pdfs/Carpenter2009.pdf18 Sep 2009: 20], but not in our experiments. As an endogenous control,. we also measured the expression of RpL32 (Rp49). ... Tsai et al. [20], we used Act88F as an endogenous control. -
Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence …
www.jiggins.gen.cam.ac.uk/pdfs/lewis2017.pdf5 Dec 2017: Virus identification and genome assembly. To identify viruses, we first mapped RNA-Seq reads to the genome of the host species to exclude genome-derived transcripts, thus filtering out endogenous viral ... Nat. Commun. 7, 13856 (2016). 9. Ghildiyal, M. -
Symbiont strain is the main determinant of variation in…
www.jiggins.gen.cam.ac.uk/pdfs/martinez2017.pdf9 Aug 2017: atpDQALL_R: 50-AATCCTTTATGAGCTTTTGC-30) relative to the. endogenous Drosophila control gene RpL32 (species-specific primers;. ... GGTTT-30; reverse: 50-CTTGAACCATGGCCTTTTG-30) relative to the. endogenous control gene RpL32 (species-specific primers; -
Wolbachia Blocks Viral Genome Replication Early in Infection without…
www.jiggins.gen.cam.ac.uk/pdfs/rainey2016.pdf26 Apr 2016: Standard curve anal-ysis was carried out and normalised to an Actin endogenous control. ... Results indicated that when comparedto Actin endogenous control there is 22 times more Wolbachia (S5 Fig), suggesting a densityof at least 100% with more than one -
1471-2156-10-75.fm
www.jiggins.gen.cam.ac.uk/pdfs/Carpenter2009b.pdf22 Feb 2010: Journalof Neurogenetics 1998, 12(4):227-240. 12. Kawamura Y, Saito K, Kin T, Ono Y, Asai K, Sunohara T, Okada TN,Siomi MC, Siomi H: Drosophila endogenous small RNAs bind -
OP-MOLB150120 2111..2129
www.jiggins.gen.cam.ac.uk/pdfs/Palmer2015.pdf10 Nov 2015: endogenous cytokine rather than directlyto pathogen molecules (see below; Leulier and Lemaitre2008). -
pgen.1002337 1..11
www.jiggins.gen.cam.ac.uk/pdfs/magwire2011.pdf4 Nov 2011: the endogenous control (actin 5C). The viral titre or geneexpression in resistant flies relative to susceptible flies was. -
pgen.1008864
www.jiggins.gen.cam.ac.uk/pdfs/lewis2020.pdf22 Apr 2021: RESEARCH ARTICLE. Widespread conservation and lineage-specificdiversification of genome-wide DNAmethylation patterns across arthropodsSamuel H. Lewis1,2,3, Laura RossID. 4, Stevie A. BainID4, Eleni PahitaID. 2,3, StephenA. SmithID. 5, Richard
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