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Reprogramming translation
https://www2.mrc-lmb.cam.ac.uk/ccsb/research/reprogramming-translation/In the first step we created an orthogonal ribosome that is selectively directed to a new orthogonal message that is not read by endogenous ribosomes. ... We first selected, on 5FU, for sequences upstream of the cat-upp fusion that do not allow the gene -
Protein labeling and imaging
https://www2.mrc-lmb.cam.ac.uk/ccsb/research/protein-labeling/Note that despite endogenous amber codons we see minimal background labeling of non-target proteins, either on the cell surface or intracellularly. -
Photochemical genetics
https://www2.mrc-lmb.cam.ac.uk/ccsb/research/photochemical-genetics/Comparison of the kinetics of ERK2–GFP nuclear accumulation following MEK photoactivation or endogenous receptor stimulation provided some interesting insights. -
https://www2.mrc-lmb.cam.ac.uk/groups/nguyen/wp-json/wp/v2/pages/5
https://www2.mrc-lmb.cam.ac.uk/groups/nguyen/wp-json/wp/v2/pages/5We develop biochemical methodsnto obtain protein-nucleic acid complexes involved in telomere maintenancenpathways both from endogenous sources and by overexpression methods. -
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/43
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/43In the first step we created an orthogonal ribosome that is selectively directed to a new orthogonal message that is not read by endogenous ribosomes. ... endogenous ribosome, creating a pool of potentially orthogonal ribosome binding sites. -
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/52
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/52the kinetics of elementary steps and adaptive feedback in kinase signalling./figcaption/figurenp /pnpComparison of the kinetics of ERK2u2013GFP nuclear accumulation following MEK photoactivation or endogenous receptor stimulation provided some -
Genetic code expansion in model organisms
https://www2.mrc-lmb.cam.ac.uk/ccsb/research/genetic-code-expansion-in-animals/The pyrrolysyl-tRNA synthetase tRNA. CUA. pair is orthogonal with respect to the endogenous synthetases and tRNAs in E. -
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/49
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/49Note that despite endogenous amber codons we see minimal background labeling of non-target proteins, either on the cell surface or intracellularly./figcaption/figurenpWe have demonstrated the efficient site-specific incorporation -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/19376
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/19376of DNA within the same strand or between opposite strands of the double-stranded helix, as a result of exposure to exogenous chemicals, such as chemotherapeutic drugs and alcohol, or endogenous -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/22
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/22{"id":22,"date":"2012-06-25T09:57:32","date_gmt":"2012-06-25T09:57:32","guid":{"rendered":"http://www2.mrc-lmb.cam.ac.uk/wordpress/?page_id=22"},"modified":"2023-07-21T12:49:25","modified_gmt":"2023-07-21T11:49:25","slug":"books","status":"publish","
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