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18 Oct 2008: a high concentration of an endogenous PDZ-binding. Figure 5: The C-termini of GluR2 and of DAT fused to thesingle transmembrane protein Tac are sufficient for BARdomain-dependent coclustering with ... This. juxtanuclear clustering strongly resembles the

2 Aug 2010: 26]. The p101 subunitprotects cells from apoptosis when overexpressed, and thismay explain why it is a common site for insertion in T-celllymphomas induced by the MoFe2-MuLV retrovirus [27].The

21 Feb 2011: Previous characterization ofBAR domain proteins has generally involved overproduc-tion, raising the need for in vivo studies characterizing theconsequences of BAR domain mutations in genes expressedat their endogenous level. ... these alterations affect

5 Aug 2013: Second, the system is typicallyorthogonal to endogenous pathways and utilizes a single definedligase. ... Furthermore, the absolute dependence on phospho-Vpu illustrates that endogenous ligases in this system do notperceive mCD4 as a misfolded protein

7 Oct 2005: he same for both Crt and Prl-Crt and were comparableo the levels of endogenous Crt (Figure 5A). ... A) Crt/ cells stably expressing either Crt-GFP or Prl-Crt-GFPwere analyzed (in duplicate) by immunoblotting with anti-Crt andanti-Sec61β (an endogenous

5 Jan 2016: ribosomes used for the cryo-tomography structure (16), thepresence or absence of heterogeneous endogenous nascentpolypeptides within Sec61 could not be determined conclusively.Thus, the conclusion that the Sec61 complex is constitutivelyopen

9 Sep 1998: We believe this effectis due to sequestration of endogenous dynamin away fromclathrin-coated pits.

11 Aug 2015: Because stable association of NEMF with 60S requires a peptidyl-tRNA (Shao et al., 2015), these complexes are likely to contain endogenous nascent chains. ... We examined this idea by analysis of endogenous RNCs on ER microsomes isolated from native

9 Dec 1999: Trans-genic (Tg) mice expressing each of these mutant PrPs in the FVB/Pmp0/0 background (null for the endogenous PrP gene) weregenerated and characterized (see Table 1 and Fig.

11 Nov 2011: This indicated that BirA has no detectable targetsendogenous to mammalian cells, and that the BioTag is notmodified by endogenous enzymes. ... Note that lowlevels of endogenous PrP are visualized at 35kDa in the SS-PrP samples probed with-PrP.

15 Sep 2011: To overcome problems caused by protein overexpression, our laboratory has used Zinc Finger Nuclease (ZFN) technology to express RFP- and GFP-tagged versions of clathrin and dynamin, respectively, preserving their endogenous

9 Sep 2015: Upon induction with tetracycline, both wild-type and mutant Sec61 transgenes (untagged) were expressed at similar levels as the endogenous protein (Figure 6—figure supplement 1C). ... Because we could not easily distinguish endogenous Sec61 from the

27 Feb 2022: resistantgenes in the endogenous RNAi background.

14 Jun 2007: Many plasma-membrane proteins are in a constant flux throughout the internal trafficking pathways of the cell. Some receptors, such as that for transferrin, are continuously internalized into recycling endosomes and returned to the cell surface.

4 Oct 2010: remove endogenous AcCoA) and used in acetyltransferaseassays.Size-exclusion Chromatography of HeLa Cytosol—The re-.

21 Sep 2006: We therefore stably expressed Crt-GFP in crt/ fibroblasts at alevel corresponding to endogenous Crt levels of matched crt/ cells(Fig.

31 Dec 2014: elifesciences.org. Szwedziak et al. eLife 2014;3:e04601. DOI: 10.7554/eLife.04601 1 of 22. Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell divisionPiotr Szwedziak†, Qing Wang†, Tanmay A M Bharat, Matthew Tsim, Jan

3 Sep 2008: Similar endogenous fila-. ments (presumably of ESCRT-III components) were observed. when an ATP-locked mutant of Vps4 (VPS4BE235Q) was overex-.

30 May 1998: m. EDTA (pH 7.4) for 30 min at0. 8. C, then with micrococcal nuclease to reduce background trans-lation products due to endogenous mRNA (29).

30 Apr 2010: Fraction 5 of the flow through stained endogenous Doc2a and -b with no remaining signal of each protein in single and double-knockout mice.

21 Dec 2004: A digitonin extract containing cytosolic proteins in-cluded the majority of endogenous NF-B (Figure 3B) aswell as several other cytosolic markers such as heat shockprotein (HSP)70 and HSP90 (our ... ng of plasmid (lane 1) was compared with endogenous

31 Aug 2006: Role of the AP2 b-Appendage Hubin Recruiting Partners for Clathrin-CoatedVesicle AssemblyEva M. Schmid. 1[, Marijn G. J. Ford. 1[, Anne Burtey. 2, Gerrit J. K. Praefcke. 3, Sew-Yeu Peak-Chew. 1, Ian G. Mills. 4,. Alexandre Benmerah2. , Harvey T.

7 Mar 2012: To clarify, we obtained two FtsA crystal structures: a) the first one contained an FtsA dimer (each FtsA molecule had the FtsZ peptide bound and had an endogenous ATP molecule at

29 Dec 2004: currently assessing the role of endogenous duck erythrocyte phosphatases in regulating the cotransporter.

11 Nov 2011: stable cell lines expressing either wild-type PrP or osteopontin (Opn)-PrP at levels comparable to endogenous PrP in brain (Kang et al., 2006).

25 Sep 2013: c) D.Mel-2 cells immunostained to reveal endogenous Syndapin (red), Tubulin ( green) and counterstained with DAPI toreveal DNA (blue). ... cleavagefurrow and their effect on cytokinesis upon overexpression inthe presence of endogenous Syndapin.

24 Jul 2020: Juszkiewicz et al., 2018; Mills et al., 2016). Here, collisions were incurred on endogenous haemo-.

6 Feb 2003: The. Jour. nal o. f Cel. l Bio. logy. JCB. Article. The Journal of Cell Biology, Volume 160, Number 4, February 17, 2003 529–539http://www.jcb.org/cgi/doi/10.1083/jcb.200210095 529. Substrate-specific function of the translocon-associated protein

21 Sep 2006: As predicted, these mice are resis-tant to prion infection and propogation41,42, consistent with a require-ment for endogenous PrPC in the replication of PrPSc.

23 Nov 2004: In the supplementary material of the previous paper we showed that these puncta co-localisedwith endogenous endocytic components such as eps15 and dynamin.

23 Sep 2011: 5C), indicating that the competitive interactionwith endogenous endophilin occurred at the membrane.

31 Jul 2019: 2011) Endogenous Myc maintains the tumormicroenvironment. Genes Dev. 25, 907916.(14) Gabay, M., Li, Y., and Felsher, D.

29 Aug 2021: Iba1) or 60 min at 37 C (anti-CD3, anti-CD4). This wasfollowed by blocking of endogenous peroxidase (peroxidase.

15 Jan 2004: Prnp0/0, except for Tg(MoPrP)4053/Prnp/, in which the endogenous Prnp genewas maintained by breeding with FVB mice (Charles River Laboratories, Hol-lister, Calif.).

n. The research team included collaborators from the MRC Centre for Medical Molecular Virology and the Retrovirus Research Laboratory, MRC University of Glasgow Centre for Virus Research.

15 Sep 2020: Cells were arrested in G1 with a-factor prior. to release into auxin and nocodazole containing media at 25˚C to deplete the endogenous Scc3.

11 Aug 2016: Published on. 11 August, 2016. HIV is a retrovirus, meaning it has to copy its RNA genome into DNA in order to infect cells.

28 Sep 2007: 1e, endogenous EHD2 in various cell lines shows asimilar peripheral distribution5,14).

16 Jul 2023: Note that using this pro-cedure, only the α-tubulin is overexpressed, and this is enough torecover functional αβ-tubulin heterodimers, as the recombinantα-tubulin associates with endogenous β-tubulin. ... The absence of endogenous, untagged

5 Jan 2024: tag endogenous genes in human cells.

17 Oct 2007: EpsinR D342R D422R overexpression in COS cells. Mutation of these two residues affects the interaction of EpsinR with clathrin. The phenotype is less severe than the lipid binding mutant (D34G R67L). pointing again to the importance of the ENTH

Comparison of the kinetics of ERK2–GFP nuclear accumulation following MEK photoactivation or endogenous receptor stimulation provided some interesting insights.

1 Aug 2010: LETTERS. A ribosome-associating factor chaperonestail-anchored membrane proteinsMalaiyalam Mariappan1, Xingzhe Li1,2, Sandra Stefanovic1, Ajay Sharma1, Agnieszka Mateja3, Robert J. Keenan3. & Ramanujan S. Hegde1. Hundreds of proteins are inserted

19 Jul 2011: Nature Reviews Molecular Cell Biology 12, 517 (2011). doi:10.1038/nrm3151

15 Apr 2004: These conclu-sions suggested that, in principle, the organization of multi-protein complexes could be assessed with antibody probes.Thus, if endogenous translocon components could be labeledwith dye-conjugated antibodies while

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Published on. 11 August, 2016. HIV is a retrovirus, meaning it has to copy its RNA genome into DNA in order to infect cells.

We develop biochemical methods to obtain protein-nucleic acid complexes involved in telomere maintenance pathways both from endogenous sources and by overexpression methods.

22 Nov 2002: EpsinR: an AP1/clathrin interacting protein. involved in vesicle trafficking. Myc-tagged and endogenous epsinR versus epsinR-GFP. ... EpsinR-GFP versus AP1 and clathrin. There is good overlap between the perinuclear epsinR-GFP and endogenous AP1 and

27 Nov 2003: Merge (from epsin1 sup data). Endogenous EpsinR. Clathrin. Merge. Comparison of epsin1 and epsinR.