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https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/15_Hegde_RS_Nature_1999.pdf9 Dec 1999: Trans-genic (Tg) mice expressing each of these mutant PrPs in the FVB/Pmp0/0 background (null for the endogenous PrP gene) weregenerated and characterized (see Table 1 and Fig. -
doi:10.1016/j.devcel.2005.09.001
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/37_Shaffer_KL_DevCell_2005.pdf7 Oct 2005: he same for both Crt and Prl-Crt and were comparableo the levels of endogenous Crt (Figure 5A). ... A) Crt/ cells stably expressing either Crt-GFP or Prl-Crt-GFPwere analyzed (in duplicate) by immunoblotting with anti-Crt andanti-Sec61β (an endogenous -
Deubiquitinases Sharpen Substrate Discrimination during Membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/82_Zhang_ZR_Cell_2013.pdf5 Aug 2013: Second, the system is typicallyorthogonal to endogenous pathways and utilizes a single definedligase. ... Furthermore, the absolute dependence on phospho-Vpu illustrates that endogenous ligases in this system do notperceive mCD4 as a misfolded protein -
Mechanism of signal sequence handover from NAC to SRP on ribosomes…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Science_2022.pdf27 Feb 2022: resistantgenes in the endogenous RNAi background. -
2168 | K. von der Malsburg et al. Molecular ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf11 Aug 2015: Because stable association of NEMF with 60S requires a peptidyl-tRNA (Shao et al., 2015), these complexes are likely to contain endogenous nascent chains. ... We examined this idea by analysis of endogenous RNCs on ER microsomes isolated from native -
Molecular Biology of the CellVol. 21, 4325– 4337, December ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/67_Emerman_AE_MBC_2010.pdf11 Nov 2011: This indicated that BirA has no detectable targetsendogenous to mammalian cells, and that the BioTag is notmodified by endogenous enzymes. ... Note that lowlevels of endogenous PrP are visualized at 35kDa in the SS-PrP samples probed with-PrP. -
Monitoring chaperone engagement of substratesin the endoplasmic…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/39_Snapp_EL_PNAS_2006.pdf21 Sep 2006: We therefore stably expressed Crt-GFP in crt/ fibroblasts at alevel corresponding to endogenous Crt levels of matched crt/ cells(Fig. -
The Acetyltransferase Activity of the Bacterial Toxin YopJ ofYersinia …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Mittal_JBC2010.pdf4 Oct 2010: remove endogenous AcCoA) and used in acetyltransferaseassays.Size-exclusion Chromatography of HeLa Cytosol—The re-. -
elife.elifesciences.org MacKinnon et al. eLife 2014;3:e01483. DOI:…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/83_MacKinnon_AL_eLife_2014.pdf9 Sep 2015: Upon induction with tetracycline, both wild-type and mutant Sec61 transgenes (untagged) were expressed at similar levels as the endogenous protein (Figure 6—figure supplement 1C). ... Because we could not easily distinguish endogenous Sec61 from the -
Journal of Lipid Research Volume 39, 1998 1287 Translocational ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/10_Rusinol_AE_JLR_1998.pdf30 May 1998: m. EDTA (pH 7.4) for 30 min at0. 8. C, then with micrococcal nuclease to reduce background trans-lation products due to endogenous mRNA (29).
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