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21 Jun 2005: To test for polymerization formation, the refoldedprotein was analyzed as described for electron microscopy.

1 Dec 2019: Circ Genom Precis Med. 2019;12:e002507. DOI: 10.1161/CIRCGEN.119.002507 September 2019 397. BACKGROUND: Pediatric cardiomyopathies are a clinically and genetically heterogeneous group of heart muscle disorders associated with high morbidity and

18 Apr 2006: through an adaptor protein. To test whether these two. complexes can directly interact in vitro, we used analyt-. ... Avanti or Echelon). Control vesicles contained an additional 10% PC. instead of the test lipid.

6 Oct 2004: Rap and PtdIns(3,4,5)P3 Activate ARAP3 Rho GAPActivity In VivoTo test whether a Rap family member activates ARAP3’sRho GAP activity in vivo, we performed more

6 Mar 2008: 74. Table 4.5: The results of the significance test (p-value < 0.05 is considered to be significant).

4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely.

11 Aug 2008: To test this, wegenerated liposomes with low curvature (800 nm in diameter)and high curvature (50 nm in diameter) and assayed binding ofArf1–GTP and Arf6–GTP to these liposomes (see

30 Apr 2010: To test the influence of EGTA and BAPTA on spontaneous release, spontaneous events were recorded first in naïve cells during 1 min, followed by a EPSC recording after stimulation. ... The spontaneous release frequencies before and after loading of a Ca2+

17 Mar 2003: amplitudes. In contrast, EPSC amplitudes of synchronous release (Geppert et al., 1994).in double mutant cells increased during the first few To test the possibility that CPX I/II deficiency causesstimuli ... Third, maximal values of synchronoussynaptic

19 Nov 2004: The fact that the domain swap is possible and a report describing two-stage unfolding kinetics of FtsZ24 prompted us to test whether the N- and C-terminal domains of FtsZ ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and

26 Nov 2012: The thin carbon film is a good phase test object (see Thon 1966) and, if the thickness of gold is correct, it displays high contrast phase detail, in which points only

19 Aug 2023: To test whether internal loop translocation occurs at the secretory translo-con, we analysed biogenesis intermediates of EAAT1, an eight-TMD protein28 with a glycosylated luminal loop after TMD3 (Fig. ... Statistical analyses in e,g were performed by

18 Dec 2008: To further test the invivo role of the Sulfolobus ESCRTcomponents, wegenerated an episomal vector to direct arabinose-inducibleexpressionof atargetgeneinS.solfataricus(21) (figs.

3 Dec 2004: Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of the test lipid (in most casesphosphatidylinositol(4,5)bisphosphate).

15 Mar 2018: thetic complex. To test this hypothesis, we examined MreB motions as we changed the shape of. ... increase with cell width (Ouzounov et al., 2016). To test if the angle of MreB movement changes.

11 Apr 2024: signalling pathway in the Drosophila midgut. Development 122, 849-858. 47. Hart K & Bienz M (1996) A test for cell autonomy, based on di-cistronic messenger.

16 Aug 2008: loading of a FtsK hexamer upon interaction with KOPS. This assay provides a good test for efficiency of loading of. ... sequently activate recombination. In order to test whether the dimeric forms of the protein had.

2 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour

27 Feb 2022: To test this, we compared the affinity ofNAC for RNCs displaying either an ER signalsequence (RNCSS) or a mutated signal sequencethat inhibits ER targeting (RNCSSmt) (fig. ... S17, B and C). Thus, thecontact between NAC UBA and SRP54 NG do-mains

17 Jan 2012: Bars and error bars correspond to mean and SEM calculated from three independent experiments (n > 250; α = 0.05; two-tailed Fisher’s exact test, , p < 0.001). ... Bars and error bars correspond to mean and SEM (n = 12–15; α = 0.05; Student’s t

23 Sep 2011: AnANOVA test confirmed that the amplitude of the fast phase wassignificantly reduced by endophilinSH3 (p 0.01). ... jneurosci.org as supplemental material), soit was important to test the specificity of its effect.

22 Jun 2001: To test this idea, we directly comparedthe translocation efficiencies of pPrl containing the pPrl, pbL, orpIgG signal sequences. ... translocation efficiency of bL-Prl and IgG-Prl. To test thishypothesis, we added either the first 5, 54, or 117 aa of the

1 Nov 2012: Acid-washed glassware was used in experiments designed to test sensitivity o f gap j u n c t i o n structure to divalent cations.

11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated.

3 Sep 2014: DLPs. We attempted to test this hypothesis directly but were. unable to obtain either individually, in combination, or expressed.

11 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,.

29 Aug 2021: outside of the sphere. We aimed to test whether Dps could efficiently dis-. ... Spike-S-Dps sera gave very little neutralisation. Whilst. pseudoviruses are widely used to test the neutralisation.

23 Dec 2013: To test whether the translocase is responsiblefor promoting and maintaining the hexameric state of FtsK invivo, we determined the stoichiometry of an FtsK derivative lack-ing the C-terminal domain (FtsKC;

22 Aug 2007: We would suggest that a good test for proteins that function ashubs only when clustered in space and time is that overexpression ofsuch a protein should not have any phenotype on

19 Apr 2002: To test whether Smc3 s head alone is sufficient. to bind Scc1, we created an artificial head in which25 min, whereas the on rate of Smc3 was about twicethat of its ... To test this directly, we repeated the aboveexperiment using a yeast strain expressing

15 Apr 2004: Each bar represents the mean SD (n 10), with statistically significant differences (P 0.01 using t test) being observed between the two conditions. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test.

21 Nov 2014: S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin ring entraps sister DNAs in a co-valent topological embrace, we isolated dimersof a

17 Jun 2005: To test if a peptide de-rived from rat amphiphysin would inhibit the amphi-physin-AP2 interaction in goldfish, we performed abinding experiment using a GST-tagged construct of ratamphiphysin 1 ... ease of vesicles that were not primed at the time

11 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,.

5 Dec 2012: To test the biological relevance of Gbg-mediated activation in p110bsignaling, we compared the ability of wild-type and mutant p110bconstructs to mediate cellular transformation and motility. ... To test the role of p110b-Gbginteractions in PTEN-null

17 Mar 2003: test set of reflections, consisting of a randomly selected 5% of the data and not used during refinement.

26 Jan 2006: 1E)(Ford et al., 2002). As many other proteins are predicted to containN‐terminal amphipathic helices, the liposome tubulation assay will provea valuable test for the folding and insertion of these

6 Apr 2017: The model was improved manually using MAIN(ref. 38) and refined with PHENIX and REFMAC (refs 39, 40), keeping the 5%randomly selected test set of reflections separate at all times.

8 Sep 2006: In tests for MT. binding, the Kar3 protein showed a Kd of 0.37 6 0.10 mM with no.

22 Aug 2007: sufficient overexpression to test if the network can be distorted and overexpressed.

22 Mar 2005: In contrast,EE/RE, was inhibited in CHC RNAi-treated cells. Welarge amounts of STxB/HRP were found in clathrin-con-used quantitative biochemical techniques to test thistaining early endosomal vacuoles ... To test whether AP1 is required for the retro-

19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.

17 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. •

2 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour

11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... To test this, we treated cultured cells with either the transla-tion elongation inhibitor cycloheximide (CHX)

8 Dec 2007: To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.

17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding.

16 Oct 2009: Future geneticstudies will allow us to test this possibility. References and Notes1.

4 Apr 2003: The best estimate was taken to be the defocus yielding the highest image Quality, Qi, de- fined as the number of test phases in agreement with (Iphase difference1 < 90’) the centrosymmetric ... 13). To test how sensitively PQ measures the signal-to-

29 Aug 2012: The crystal structure of the complex between BETA and BLIP has been a test case for molecular replacement because of the difficulty encountered in the original structure solution.