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Spin assays for lipid binding and recruitment
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/spin/spin.html25 Jun 2007: -
Monolayer Assay Protocol
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/mono.html25 Jun 2007: As a negative control, inject pure chloroform without any lipid (this will test for lipid dependence of any structures seen, such as whether clathrin baskets form in solution instead of clathrin -
HARVEY T McMAHON
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/people/CV2006d.html25 Jun 2007: We then collaborated with Peter Cullen (Bristol University) to test the in vivo function of sorting-nexin1 in membrane trafficking. ... The model stands the test of time but we would still like a direct measurement of the force produced by GTP hydrolysis. -
DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf6 Apr 2007: Fur-ther work will be required to test this prelim-inary interpretation. Existing data indicates that ATP binding hasprofound effects on the kinesin head, recon-figuring the active site and shifting -
doi:10.1016/j.str.2007.05.002
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf2 Jun 2007: protein saturation on liposomes. To test more directly whether the dimer seen in the crys-. ... tutive helix. To test for a role for helix 1 in membrane bind-. -
Structural analysis of the ParR/parC plasmidpartition complex Jakob…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MollerJensen_ParR_EMMBO_2007.pdf4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely. -
ANALYSIS Integrating molecular and network biologyto decode…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007.pdf22 Aug 2007: We would suggest that a good test for proteins that function ashubs only when clustered in space and time is that overexpression ofsuch a protein should not have any phenotype on -
Supplementary Text & Figs
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007Sup.pdf22 Aug 2007: sufficient overexpression to test if the network can be distorted and overexpressed. -
LETTERS Structural basis for selective recognition of ESCRT-IIIby the …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17928861.pdf8 Dec 2007: To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2. -
tra_578 1..18
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Burtey%20IVF%20Traffic%2007.pdf15 May 2007: To directly test the. role of each residue of the IVF motif, in the context of. ... Methods. Errors bars were obtained by calculating the standard error and relevance of the difference assessed with a Student t-test.
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