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Endocytic Interactome: How Interactomes can help us design…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/Interactomes/index.html26 Sep 2011: What is endophilin and how does it work. Research on the mechanism of endophilin in rapid vesicle endocytosis. Research on proteins involved in clathrin-coated vesicle formation. Research on membrane bending. Research on protein-lipid interactions. -
YopJ activation by IP6
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/Black_Plague/YopJ_IP6.html26 Sep 2011: Researching the reason immune responses are sprroessed during Yersinia infections. -
Christian Münch Receives British Neuroscience Association…
https://www2.mrc-lmb.cam.ac.uk/christian-munch-receives-british-neuroscience-association-postgraduate-award/
15 Dec 2011:
During his PhD work, Christian studied what causes aggregation of mutant SOD1 in the test tube and in cells.
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Isothermal titration calorimetry to measure protein/peptide/lipid…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/ITC.html26 Sep 2011: Isothermal Titration Calorimetry is used to make accurate measurements of affinities and stoichiometries of interactions. It is based on fine measurements of heat changes when ligands interact -
McMahon Group techniques
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/techniqs.html26 Sep 2011: Overexpression of endocytic proteins in COS cells to test the effects of mutations in vivo. ... Liposome/lipid nanotube sedimentation assays to test lipid binding and specificity. (also immunoprecipitations, cloning, kinase assays, etc.). -
Clathrin and its interactions with AP180. AP180 is a brain enriched…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/AP180/Clathrin.html26 Sep 2011: More experiments need to be done to test this. The platinum shadowing (from a single angle) shows that AP180 does not induce curvature in clathrin assemblies (left panel). -
Molecular Biology of the CellVol. 21, 3054 –3069, September ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/RVS161_MBC2010.pdf21 Feb 2011: To test these activities, wecoexpressed full-length Rvs161 and Rvs167 using an E. ... To-gether, our biochemical tests show that Rvs161-Rvs167 bindsto membranes and induces three-dimensional curvature. -
Biochem. J. (2011) 440, 185–193 (Printed in Great Britain) ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/ChernomordikBJ2011.pdf15 Nov 2011: kDa dextran were significantly different (paired Student’s t test, P < 0.001,n = 6). ... We used two experimental approaches, transfection andmicroinjection, to test the effects of this domain on HA-initiatedsyncytium formation. -
A Conserved Archaeal Pathway for TailAnchored Membrane Protein…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/70_Sherrill_J_Traffic_2011.pdf11 Nov 2011: To test this, we first determinedthe X-ray crystal structure of ADP•AlF4-bound TRC40from the methanogen Methanothermobacter thermau-totrophicus at 2.1 Å resolution (Table S1, Figure S1 andMethods). ... To test this, we used a protease-protection -
Cellular/Molecular Endophilin Drives the Fast Mode of Vesicle…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Llobet%20J%20Neurosci2011.pdf23 Sep 2011: AnANOVA test confirmed that the amplitude of the fast phase wassignificantly reduced by endophilinSH3 (p 0.01). ... jneurosci.org as supplemental material), soit was important to test the specificity of its effect.
15 Dec 2011:
During his PhD work, Christian studied what causes aggregation of mutant SOD1 in the test tube and in cells.
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