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doi:10.1016/j.molcel.2006.07.020
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/HiroseMC2006.pdf8 Sep 2006: In tests for MT. binding, the Kar3 protein showed a Kd of 0.37 6 0.10 mM with no. -
Supplementary Text & Figs
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007Sup.pdf22 Aug 2007: sufficient overexpression to test if the network can be distorted and overexpressed. -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&refresh=669d2bcc7ab2617215763962 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour -
Statistical Theory; Why is the Gaussian Distribution so popular? ...
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/lectures/Lecture4_Statistical_Theory.pdf17 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. • -
PII: S1534-5807(04)00100-5
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Johannes_DevCell2004.pdf22 Mar 2005: In contrast,EE/RE, was inhibited in CHC RNAi-treated cells. Welarge amounts of STxB/HRP were found in clathrin-con-used quantitative biochemical techniques to test thistaining early endosomal vacuoles ... To test whether AP1 is required for the retro- -
Cooperative Recruitment of Dynamin and BIN/Amphiphysin/Rvs (BAR)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/JBC_Meinecke2013.pdf19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question. -
LETTERS Structural basis for selective recognition of ESCRT-IIIby the …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17928861.pdf8 Dec 2007: To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2. -
2168 | K. von der Malsburg et al. Molecular ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... To test this, we treated cultured cells with either the transla-tion elongation inhibitor cycloheximide (CHX) -
Molecular Replacement Practical IntroductionDuring the practical you…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/tutorials/MolecularReplacementPractical.pdf29 Aug 2012: The crystal structure of the complex between BETA and BLIP has been a test case for molecular replacement because of the difficulty encountered in the original structure solution. -
Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding. -
early in DNA damage response and regulateRAP80 localization. Many ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MartensSytPap07.pdf16 Oct 2009: Future geneticstudies will allow us to test this possibility. References and Notes1. -
PII: S0304-3991(97)00070-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram97.pdf4 Apr 2003: The best estimate was taken to be the defocus yielding the highest image Quality, Qi, de- fined as the number of test phases in agreement with (Iphase difference1 < 90’) the centrosymmetric ... 13). To test how sensitively PQ measures the signal-to- -
mmi_4133.fm
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Spo0J%202004.pdf30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel -
Functional Depletion of Mahogunin by Cytosolically Exposed Prion…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/59_Chakrabarti_O_Cell_2009.pdf13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-. -
A substrate-specific inhibitor of proteintranslocation into the…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/35_Garrison_JL_Nature_2005.pdf15 Jul 2005: 2e). To test whether this difference underlies cotransin’ssubstrate selectivity, we prepared chimaeric constructs in which thesignals of VCAM1 and pPrl were fused to an otherwise non-translocated reporter. ... 3a), these results suggestthat cotransin -
PNAS202120006_proof
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas%20Lee%202022.pdf18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig. -
doi:10.1016/j.tibs.2009.03.001
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/58_Chakrabarti_O_TIBS_2009.pdf13 Jun 2009: It will then be possible to test in transgenicmice whether the effects of the HD mutant stem solely fromCtmPrP production or through other mechanisms (e.g. ... PrP gener-ation, trafficking and degradation so that they can bemanipulated selectively to -
PHAGOCYTES, GRANULOCYTES, AND MYELOPOIESIS PSTPIP2 deficiency in mice …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Blood-2012-Chitu-3126-35.pdf12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... To test the impact of PSTPIP2-deficiency disease in mice onosteolytic responses in vivo, we performed -
No Job Name
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/vandenent2008ftsq.pdf4 Mar 2008: Expression tests were done inBl21AI cells (Invitrogen) and C41 cells (Miroux and Walker,1996) with a 10 ml culture and purified over a Ni2 NTA spincolumn as described previously (van den -
Arginine 383 is a crucial residue in ABCG2 biogenesis
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/56_Polgar_O_BBA_2009.pdf13 Jun 2009: 2.4. Northern blotting. RNA was extracted from cells using RNA STAT-60 (Tel-Test Inc.,Friendswood, TX). ... 2A). To test the functionality of the R383A and R383G mutants, flowcytometry was performed after incubating cells with the ABCG2-substrates
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