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19 Nov 2004: 2). The break in the protofilament is necessary for crystallization because the dimer formed by the 43-Å interaction (middle of Fig. ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and after centrifugation nucleotide

3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. ... Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of

13 Dec 2008: inclusion reaching its middle (Fig. 4 b) will not result in themembrane bending since the upper monolayer can expand. ... 5). To test the sensitivity of the model. predictions to this assumption, we repeated the calculations.

22 Jul 2007: F-actin stress fibres (Figure 2E, middle panel compared with. uninfected cells or cells infected with retrovirus expressing. ... In addition,. using mouse models as a preclinical test, as we have done in.

12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... Pseudocolored fluorescence image ofCOS-7 cells expressing EGFP alone (left), WT EGFP-PSTPIP2 (middle) or

16 Jul 2023: S1E). As a further test of thisfinding, we expressed the Vps4E209Q plasmid in a cell line carryinga thymidine kinase homolog (hereafter called STK). ... 3A).To test the functional significance of this patterned assembly ofESCRT-III polymers, we used

14 Jun 2006: Middle) Plot of the similarity between PI3-Ks according to their IC50 values against small molecule. ... cell culture are recapitulated in vivo by performing insulin. tolerance tests in mice.

21 Nov 2014: Scc1 contains many more residues in the middle domain. Separase cleavage sites, Pds5(7) and Scc3 binding sites are highlighted (28). ... S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin

18 Feb 2016: Thus, the idea emerged that mRNAs are ‘tested’ for their integrity by translation, and failure of this test initiates their degradation to prevent production of faulty proteins1,31. ... An advantage of encoding an arrest-inducing sequence in the

4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely.