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McMahon Group techniques
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/techniqs.html26 Sep 2011: Overexpression of endocytic proteins in COS cells to test the effects of mutations in vivo. ... Liposome/lipid nanotube sedimentation assays to test lipid binding and specificity. (also immunoprecipitations, cloning, kinase assays, etc.). -
Endocytic Interactome: How Interactomes can help us design…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/Interactomes/index.html26 Sep 2011: What is endophilin and how does it work. Research on the mechanism of endophilin in rapid vesicle endocytosis. Research on proteins involved in clathrin-coated vesicle formation. Research on membrane bending. Research on protein-lipid interactions. -
Clathrin and its interactions with AP180. AP180 is a brain enriched…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/AP180/Clathrin.html26 Sep 2011: More experiments need to be done to test this. The platinum shadowing (from a single angle) shows that AP180 does not induce curvature in clathrin assemblies (left panel). -
YopJ activation by IP6
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/Black_Plague/YopJ_IP6.html26 Sep 2011: Researching the reason immune responses are sprroessed during Yersinia infections. -
Isothermal titration calorimetry to measure protein/peptide/lipid…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/ITC.html26 Sep 2011: Isothermal Titration Calorimetry is used to make accurate measurements of affinities and stoichiometries of interactions. It is based on fine measurements of heat changes when ligands interact -
Volume 22 May 15, 2011 1625 Cytosolic aggregates perturb ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/69_Chakrabarti_O_MBC_2011.pdf11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated. -
Molecular Biology of the CellVol. 21, 3054 –3069, September ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/RVS161_MBC2010.pdf21 Feb 2011: To test these activities, wecoexpressed full-length Rvs161 and Rvs167 using an E. ... To-gether, our biochemical tests show that Rvs161-Rvs167 bindsto membranes and induces three-dimensional curvature. -
Protein-driven membrane stresses in fusion and fission
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovCurvature2010.pdf21 Feb 2011: middle of the stalk and contributes similarly to theenergies of both fusion and fission stalks [3,73].[(Box_1)TD$FIG]. ... Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test. -
Membrane Protein Insertion at the Endoplasmic Reticulum
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/75_Shao_S_Ann_Rev_2011.pdf9 Nov 2011: The apparentfree-energy contribution of each amino acidin any test sequence was found to be additive,with the sum determining the final insertionprobability. ... earlier transmembrane domains ( gray), and a later test sequence(red ) is measured for -
JCB: Article The Rockefeller University Press $30.00J. Cell Biol. ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/J%20Cell%20Biol%202010%20Howes.pdf24 Aug 2011: A single section of the original tomogram is shown (left). Various rotations of a 3D contoured electron density render were generated (middle). ... 6 F). To test the requirement for CLIC endocytosis during mi-gration, this inactivation assay was applied
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