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21 Dec 2004: Between 5000 and 10,000 cells were plated per well of a 96-well platebetween 24 and 36 h before transfection, at which time they were 40 – 80%confluent. ... Unless other-wise noted in the figure legend, the cells were analyzed 18 –24 h after

15 Apr 2004: 22|i-j|24) 78 Weak (0–5.0 Å) 183Long range (|i – j| > 4) 163Total 554 (=7.1 per residue)Statistics for 45 accepted structures:. ... J ApplCrystallogr 24: 946–950. Kuroda, A., Asami, Y., and Sekiguchi, J.

1 Nov 2004: Multiplicity (last shell) 14.1 (14.1) 14.3 (14.2) 11.6 (11.6) 7.2 (7.2) 6.9 (3.9) 24.9 (24.0)I/ (last shell)

2 Feb 2004: Membrane transport between compartments in eukary-otic cells requires proteins that allow the budding andSCISSION of nascent cargo vesicles from one compartmentand their targeting and fusion with another. Just asSNARES are proposed to be essential

23 Nov 2004: Transferrin uptake and immunofluorescenceCos 7 cells were transfected using GeneJuice according to manufacturers instructions,plated onto polylysine coated coverslips 24 hours post transfection and assayed fortransferrin uptake 48 hours post

30 Jan 2004: recruit clathrin to membranes. Mammalian am-phiphysin2 has several clathrin-binding sites (24–26), and we found that the wild-type, but notmut1, efficiently recruited clathrin to liposomes(fig. ... It is interesting to note that thestructure of the

15 Apr 2004: Sec61 Sec61 21.4 3.6 24.8 6.1a 23.9 2.5a. FRET efficiencies (mean SD; n 40) for the indicated donor–acceptorantibody pairs were measured on cells that

29 Dec 2004: rg/ ml for 24 h at 37 “C).

23 Nov 2004: Evolving nature of the AP2 a-appendage hubduring clathrin-coated vesicle endocytosis. Gerrit JK Praefcke1,2, Marijn GJ Ford1,Eva M Schmid, Lene E Olesen,Jennifer L Gallop, Sew-Yeu Peak-Chew,Yvonne Vallis, M Madan Babu,Ian G Mills3 and Harvey T

19 Nov 2004: Biol. Chem. 276, 17307–17315 (2001). 24. Andreu, J.M., Oliva, M.A. & Monasterio, O. ... J. Appl. Crystallogr. 24, 946–950 (1991). 41. Miroux, B. & Walker, J.E.