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News - Cambs 24
https://www2.mrc-lmb.cam.ac.uk/archive/articles/Road_named_after_legendary_scientist.pdf1 Feb 2013: Copyright 2013 Archant Regional Ltd. All rights reserved. News - Cambs 24 http://www.cambs24.co.uk/news/cambridge_road_to_be_na. -
Bacterial chromosome segregation: structure andDNA binding of the Soj …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/soj_embo2005.pdf30 Jan 2005: The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization. ... The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization272. -
Pi-PEG Screen 1.5 disclaimer
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB19.pdf28 Sep 2022: 6.45 % 1-23 PEG 600 25.65 % BICINE 75 mM 8.4 PEG 2000 MME 6.45 % 1-24 PEG 1000 22.5 % BICINE 75 mM 8.8 PEG 3000 ... 8 PEG 3000 12.9 % 1-35 PEG 200 24.15 % BICINE 75 mM 8.4 PEG 4000 10.65 % 1-36 PEG 300 21.45 % BICINE 75 mM 8.8 -
Structure of a Bacterial Dynamin-like Protein Lipid Tube Provides a…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cell%202009%20dynamin.pdf19 Dec 2009: ell 139, 1342–1352, December 24, 2009 ª2009 Elsevier Inc. 1347. A B. ... at either end. ell 139, 1342–1352, December 24, 2009 ª2009 Elsevier Inc. -
Manual_CO-311 JBScreen Solubility HTS
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/SOLUB.pdf19 Mar 2018: Description of JBScreen Solubilty HTS The screen contains 24 conditions that differ by buffer chemicals and by pH. ... These 24 conditions are presented 4 times in the screen: with the buffer components alone, with 150 mM NaCl, with 5% glycerol, and with -
bic079a
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DonaldsonJ.pdf11 Aug 2008: In cells, the Arf GTP-bind-ing and hydrolysis cycle is regulated by GEFs (guanine-nucleo-tide-exchange factors) and GAPs (GTPase-activating proteins).There are 14 GEFs and 24 GAPs ... EMBO J. 24,2244–2253. 10 Peter, B. J., Kent, H. M., Mills, I. -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB12.pdf2 Oct 2014: Section D: Conditions (16 -18) and (22 - 24) supplements the CSS-I with other PEGS mixed with KSCN. • ... 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. -
Expression of selenomethionine substituted proteins innon-methionine…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/SeMet%20recipe.doc.pdf2 Feb 2004: J Mol Biol. 1993 Jan 5;229(1):105-24. The protein is expressed in any E. -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: Genetics 159, 133–145 (2001). 24. Gaffen, S. L. Recent advances in the IL-17 cytokine family. ... Optogenetics. L4 animals expressing ChR2 in RMG27 were picked 24 h before the assay and divided into two groups. -
Addit05_NeXtal pH gradient
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-05.pdf20 Nov 2020: 1 MD4 22 6.4 Sodium Cacodylate 1 MD5 23 6.6 Sodium Cacodylate 1 MD6 24 6.8 Sodium Cacodylate 1 ME1 25 7 Sodium HEPES 1 ME2 26 -
Structure of a cholinergic cell membraneNigel Unwina,1 Edited by ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS22.pdf4 Nov 2022: The broken yellow lines identify the well-documented ribbon of δ subunit-linked receptor dimers(14, 15, 18, 24), one of which is labeled in each leaflet to indicate the subunit ... Biophys. J. 92, 3960–3967 (2007). 24. H. W. Chang, E. Bock, Molecular -
1 Clear StrategyTM Screen I MD1-14 A 6 × ...
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB10.pdf2 Oct 2014: MD1-14 is presented as a 24 x 10† mL stock conditions 2 x 10 mL of 5 different pH’d buffers. ... 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. -
Molecular basis for membraneremodelling and organization Deadline for …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/F-BAR_proteins/Le%20Poster_DEUXNew.pdf29 Sep 2011: 24-28 September 2011. Main Topics:Membrane curvature invesicle fusion. Membrane curvature in endocytosis and vesicle budding. -
Addit04b_AL-SEL-DP Formated
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-04b.pdf24 Nov 2020: FOS-CHOLINE-UNSAT-11-10 31 FC-U10-11 FCU110 6.2 335.4B 12 24 1,2-Diheptanoyl-sn-glycero-3-phosphocholine 8.4 DHPC D607 1.4 481.5C ... OHES Bachem - P1105 24.3 n/aG 2 74 CYMAL-3 120 CYMAL-3 C323 30 466.5G 3 75 CYMAL-4 38 CYMAL-4 C324 7.6 480.5G 4 -
S413_6a 39..44
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreB%20Nature.pdf30 Aug 2001: 51.1 Å. IA. IBIIB. IIA. F-actin MreB. 55 Å. 1. 24. ... Interestingly, the rened model ofF-actin23,24 is less similar to our crystal structure than the initialunrened model, in which the crystal structure of G-actin incomplex with DNase I was -
MD1-98 The LMB Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB21.pdf15 Jul 2016: Tris propane 7.11-22 24 % v/v Ethanol 0.1 M HEPES 7.8 0.04 M Magnesium chloride hexahydrate1-23 17.5 % v/v Ethanol1-24 1.3 M ... M Sodium chloride1-43 15 % w/v PEG 3350 0.1 M MES 6.21-44 24 % w/v PEG 3350 0.05 M HEPES 6.8 0.15 M Sodium -
Filament structure and subcellular organization of the bacterial…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Liu_PNAS_crescentin_2024.pdf28 Apr 2024: This is reminiscent of eukaryotic intermediate filaments (IFs), which are also known to be non- polar (21–24). ... mass spectrometry anal-yses of IF protein oligomers, and recent cryo- EM analyses (21–24,. -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB11.pdf2 Oct 2014: 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. ... Section D: Conditions (16 -18) and (22 - 24) supplements the CSS-I with other PEGS mixed with KSCN. • -
MD1-46 Morpheus
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB23.pdf4 Aug 2021: 1-21 B9 - - - 0.24 M Ethyleneglycol 0.1 M Buffer System 2 7.5 30 % Precipitant Mix 2. ... 1-24 B12 0.8 % Alkaloids 0.12 M Ethyleneglycol 0.1 M Buffer System 3 8.5 30 % Precipitant Mix 1. -
doi:10.1016/j.cub.2004.09.077
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Snx1PeteCullen2004/Snx1PeteCullen2004.pdf23 Nov 2004: Curr. Opin.Cell Biol. 14, 454–462. 24. Seaman, M.N.J., Marcusson, E.G., Cereghino, J.L., and Emr,S.D. -
MD1-91, MD1-92 and MD1-92-FX Morpheus II 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB20.pdf9 Jul 2018: L-rhamnose monohydrate Monosaccharide RAM 43. DL-threonine Amino-acid DTH; THR 23;n/a. DL-histidine, HCl, H20 Amino-acid DHI; HIS 24; n/a. ... v Precipitant Mix 61-23 B11 2 mM Divalents II 0.1 M Buffer System 6 8.5 30 % v/v Precipitant Mix 71-24 B12 2 mM -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: JBS6 JenaBioScience CS-106L 24 Incomplete factorial based on AmS (pH 4.6-8.5). ... JBS7 JenaBioScience CS-107L 24 Incomplete factorial based on MPD (pH 4.6-8.5). -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/sedequil.pdf16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at -
MD1-116-117 Morpheus III-Morpheus III HT-96
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB22.pdf9 Jul 2018: 3 8.5 30 % Precipitant Mix 21-23 B11 1.5 % Vitamins mix 0.1 M Buffer System 3 8.5 30 % Precipitant Mix 31-24 B12 1.5 % Vitamins mix ... 8.5 30 % Precipitant Mix 32-24 F12 0.6 % Antibiotics mix 0.1 M Buffer System 3 8.5 37.5 % Precipitant Mix 42-25 G1 1.2 -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-03.pdf9 Jul 2018: D3 21 1.5 % Caffeic acid †D4 22 1.5 % D-()-Quinic acidD5 23 1.5 % Gallic acid monohydrate†D6 24 1.5 % N-vanillylnonanamide (capsaicin synthetic)†E1 25 1.5 % -
MD1-46, MD1-47 and MD1-47-FX Morpheus 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB17.pdf9 Jul 2018: 5 30 % v/v Precipitant Mix 31-24 B12 0.09 M Halogens 0.1 M Buffer System 3 8.5 37.5 % v/v Precipitant Mix 41-25 C1 ... 0.12 M Monosaccharides 0.1 M Buffer System 3 8.5 30 % v/v Precipitant Mix 32-24 F12 0.12 M Monosaccharides 0.1 M Buffer System 3 -
Crystal Screen Lite™ is a complete reagent kit designed ...
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB06b.pdf26 Jun 2015: Please e-mail, fax, or telephone your request to Hampton Research. Fax and e-mail Technical Support are available 24 hours a day. ... 24. 0.2 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 10% v/v 2-Propanol. -
Impress
https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf28 Sep 2020: Aids). 23 / 50. Screenshots. 24 / 50. Generic Objects. 25 / 50. ... Slide 24. Slide 25. Slide 26. Slide 27. Slide 28. Slide 29. -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/stock2005robot.pdf19 Jan 2005: Quik phosphate grid screen 24 Hampton Research. NaCl grid screen 24 Hampton Research. ... The platform also accommodates most commercially availablecrystallisation plates (24-well Linbro (Hampton Research, #HR3-110), 24-well Cryschem(Hampton Research, -
mk069902063p
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Fitting.MBC.pdf26 May 1999: J. Appl. Crystallogr. 24, 946 –950. Kull, F.J., Sablin, E.P., Lau, R., Fletterick, R.J., and Vale, R.D. -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/sedequil.pdf16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at -
MD1-93 the Morpheus Additive Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-01.pdf5 Jul 2018: L-rhamnose monohydrate Monosaccharide RAM 43. DL-threonine Amino-acid DTH; THR 23;n/a. DL-histidine, HCl, H20 Amino-acid DHI; HIS 24; n/a. -
PII: S0014-5793(01)02216-5
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinD%20FEBS.pdf5 Mar 2001: 1990) Annu.Rev. Genet. 24, 249274. [3] Lutkenhaus, J. and Addinall, S.G. ... Appl. Crystallogr. 24, 946950.[34] Merritt, E.A. and Bacon, D.J. (1997) in : Macromolecular Crys-. -
2-126 Formulations
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB05.pdf2 Oct 2014: Tube Number pH. 1. 7.12. 7.23. 6.24. 6.75. 5.86. 6.97. 5.18. -
Microscopy, 2021, 1–6doi:https://doi.org/10.1093/jmicro/dfab023…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/mic21.pdf26 Jul 2021: acetylcholine receptor clusters in C2C12 myotubes. J. Lipid. Res. 47:2121–2123. 24. -
Sup Fig 1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJSup.pdf11 Nov 2008: 24 h after transfection cells were serum-starved for 16 h and then stimulated with 20 ng/ml TNF-α. ... 24. 26. 28. 30. 32. 34. 36. 38. 40. 42. inte. -
KWRM_A_992668_O
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-3.pdf20 Oct 2015: Submitted: 11/06/2014. Revised: 11/21/2014. Accepted: 11/24/2014. http://dx.doi.org/10.4161/21624054.2014.992668This is an Open Access article distributed under theterms of the ... Molecular anatomy of a trafficking organelle.Cell 2006; 127:831-46; -
Nature © Macmillan Publishers Ltd 1998 8 30. Fan, ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsZ.Nature.pdf9 Jan 1998: Biochem. 24, 255–302 (1995). 22. Leslie, A. G. W. Recent changes to the MOSFLM package for processing film and image plate data (SERCLaboratory, Daresbury, Warrington, UK, 1991). ... 23. The CCP4 suite: Programs for protein crystallography. Acta -
1 CURRICULUM VITAE Venki Ramakrishnan Nationality U.S. (since 1985)…
https://www2.mrc-lmb.cam.ac.uk/groups/ribo/download/ramakrishnan_cv_4.pdf4 Jan 2024: Nat Struct Mol Biol 24, 866-869. N. R. James, A. Brown, Y. ... 123, 24-34. Gerchman, S.E. and Ramakrishnan, V. (1987). Chromatin higher-order structure studied by neutron scattering and scanning transmission electron microscopy. -
A Large Particle Associated with the Perimeter of the ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb82.pdf5 Jul 2010: Best preserved porecomplexes show evidence of 16- and 24-fold harmonics inaddition to the basic eightfold harmonic (Fig. ... J. CellBiol. 52:338-354. 24. Paine, P. L. , L, C. Moore, and S. -
ELIFE07975 1..21
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/94_Voorhees_RM_eLife_2015.pdf11 Aug 2015: By contrast, TMDs responsible for targeting aremarkedly more hydrophobic and between 17 to 24 residues long. -
Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AlfA2018.pdf20 Mar 2018: The subunits are staggered in a left-handed, double-heli-cal, parallel (polar) filament, with a rise of 24.9 Å and a 156.5 twist. ... Science 338:1334–1337. 24. Schumacher MA, et al. (2007) Segrosome structure revealed by a complex of ParRwith -
Structure of bacterial tubulin BtubA�B:Evidence for horizontal gene…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/btubab%202005.pdf21 Jun 2005: BtubAB form the same het-erodimer as tubulin (24, 25). The protofilament axis isvertical. ... 1993) Micron 24, 465– 471. 10. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & -
�������� ��� � ���� �� �� ���� ��� ������ ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsA%20EMBO%20.pdf4 Oct 2000: E#N# 04412 (B<-! A # # " 3 16G7H#. # >$$I# ># 3 J#3# 04492 ' ( ' ) % % # ) &. 2'99G997#. $# B # O (# 0442 ( (- $ $ 6H $ # # / 0/ 24 64HG647#. (#-# -
doi:10.1016/j.jmb.2004.05.031
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/zapa%202004.pdf16 Aug 2004: In vitro, FtsZ isextremely versatile, forming protofilaments, thickfilaments, sheets,21,22 helical tubes23,24 and asters.25. ... FEMS Microbiol. Rev. 24,531–548. 13. Rothfield, L., Justice, S. & Garcia-Lara, J.(1999). -
se289900215p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/strucrev.pdf6 Jul 1999: Adaptors EM, rotary shadow and deep-etch; APcomplexes (13). ;25 (2D). Crystallography; AP2 m2 residues 158 to435 (24). ... 2.7 Phe174, Arg423 (24) YxxØ endocytosis signals. Crystallography; AP2 COOH-terminala-adaptin appendage domain residues701 to 938 -
Mechanisms of Dense Core Vesicle Recapture following “Kiss andRun” ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Insulin_Tsuboi_JBC2004/Insulin_TsuboiJBC2004.pdf6 Dec 2004: Received for publication, July 20, 2004, and in revised form, August 24, 2004Published, JBC Papers in Press, August 25, 2004, DOI 10.1074/jbc.M408179200. ... MIN6 Cell Culture—MIN6 -cells (24) were used between passages19 and 30 and grown in -
Crystal structure of the SOS cell division inhibitorSulA and ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SulA%20PNAS%202003.pdf13 Jun 2003: To whom correspondence should be addressed. E-mail: jyl@mrc-lmb.cam.ac.uk. www.pnas.orgcgidoi10.1073pnas.1330742100 PNAS June 24, 2003 vol. ... Lowe, J. (1999) Struct.Folding Des. 7, 1181–1187. 24. Van Duyne, G. -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf3 Dec 2004: 24). Thus our experiments point to a pioneering role forepsin in vesicle budding as it provides both a driving force and a linkbetween membrane invagination, clathrin polymerization and AP2complex recruitment. ... beta 2-adaptin appendage domain. EMBO J. -
PII: 0304-3991(84)90197-9
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram84.pdf26 Oct 2008: The capacity of the Dewar is sufficient to give a running time of about 24 h under these conditions.
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