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Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/20130430_Cryst_course.pdf2 May 2013: Acta Crystallogr. D Biol. Crystallogr. 60, 203–204 (2004). Solubility Screen. Protein characteriza0on 1-‐10mg/ml 100nl/drop Incubate 1-‐24 hrs. ... Or Jena Bioscience JBScreen Solubility HTS. Solubility Screen. Protein characteriza0on 1-‐10mg/ml -
pone.0052401 1..15
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Podosome2012/journal.pone.0052401.pdf10 Jan 2013: clear amphipathic characteristics and fold on membrane binding. [4]. A 24 and 28 amino acid long helix, H0, would be possible for. ... Members of the PAK family are major. effectors of the Rho GTPases CDC42 and Rac (reviewed in [24]). -
Slide 1
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/processing.pdf13 May 2013: approach. 24/44. 24/44. Integration by profile fitting. Based on the assumption that spots corresponding to fully recorded reflections in the same region of the detector (and on images nearby -
Kevin Cowtan, cowtan@ysbl.york.ac.uk 2012 Automated phase…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/densitymod12-ppt.pdf28 May 2013: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&refresh=66968e553e86817211428692 May 2013: Acta Crystallogr. D Biol. Crystallogr. 60, 203–204 (2004). Solubility Screen. Protein characteriza0on 1-‐10mg/ml 100nl/drop Incubate 1-‐24 hrs. ... Or Jena Bioscience JBScreen Solubility HTS. Solubility Screen. Protein characteriza0on 1-‐10mg/ml -
Supplement_Final
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/81_Shao_S_Mol_Cell_2013.pdf16 May 2013: Molecular Cell. Article. Listerin-Dependent NascentProtein Ubiquitination Relieson Ribosome Subunit DissociationSichen Shao,1,2 Karina von der Malsburg,1,2 and Ramanujan S. Hegde1,1MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/pathologies_and_twinning.pdf11 Jun 2013: 04/06/2013 Crystallography Course LMB 24. (Pseudo)merohedral twining is impossible. 432 622. ... Data processing ( -‐1 ). Molecular replacement ( P1 ). Refinement ( P1 ). R / R-‐free = 0.24 / 0.31. PDB: 1yup ( P1 ). PDB: 1yup. Zanuda. ( P21 ) -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&refresh=66968e562c474172114287011 Jun 2013: 04/06/2013 Crystallography Course LMB 24. (Pseudo)merohedral twining is impossible. 432 622. ... Data processing ( -‐1 ). Molecular replacement ( P1 ). Refinement ( P1 ). R / R-‐free = 0.24 / 0.31. PDB: 1yup ( P1 ). PDB: 1yup. Zanuda. ( P21 ) -
Kevin Cowtan, cowtan@ysbl.york.ac.uk 2012 Automated phase…
https://www2.mrc-lmb.cam.ac.uk/download/automated-phase-improvement-and-model-building-kevin-cowtan-cowtanysbl-york-ac-uk/?wpdmdl=18786&refresh=66968e565926c172114287028 May 2013: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. -
Slide 1
https://www2.mrc-lmb.cam.ac.uk/download/integration-and-scaling-harry-powell-mrc-lmb-crystallography-course-10th-may-2013/?wpdmdl=18796&refresh=66968e55ae5a8172114286913 May 2013: approach. 24/44. 24/44. Integration by profile fitting. Based on the assumption that spots corresponding to fully recorded reflections in the same region of the detector (and on images nearby
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