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Expression of selenomethionine substituted proteins innon-methionine…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/SeMet%20recipe.doc.pdf2 Feb 2004: J Mol Biol. 1993 Jan 5;229(1):105-24. The protein is expressed in any E. -
Pi-PEG Screen 1.5 disclaimer
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB19.pdf28 Sep 2022: 6.45 % 1-23 PEG 600 25.65 % BICINE 75 mM 8.4 PEG 2000 MME 6.45 % 1-24 PEG 1000 22.5 % BICINE 75 mM 8.8 PEG 3000 ... 8 PEG 3000 12.9 % 1-35 PEG 200 24.15 % BICINE 75 mM 8.4 PEG 4000 10.65 % 1-36 PEG 300 21.45 % BICINE 75 mM 8.8 -
Molecular basis for membraneremodelling and organization Deadline for …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/F-BAR_proteins/Le%20Poster_DEUXNew.pdf29 Sep 2011: 24-28 September 2011. Main Topics:Membrane curvature invesicle fusion. Membrane curvature in endocytosis and vesicle budding. -
Bacterial chromosome segregation: structure andDNA binding of the Soj …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/soj_embo2005.pdf30 Jan 2005: The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization. ... The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization272. -
Manual_CO-311 JBScreen Solubility HTS
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/SOLUB.pdf19 Mar 2018: Description of JBScreen Solubilty HTS The screen contains 24 conditions that differ by buffer chemicals and by pH. ... These 24 conditions are presented 4 times in the screen: with the buffer components alone, with 150 mM NaCl, with 5% glycerol, and with -
bic079a
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DonaldsonJ.pdf11 Aug 2008: In cells, the Arf GTP-bind-ing and hydrolysis cycle is regulated by GEFs (guanine-nucleo-tide-exchange factors) and GAPs (GTPase-activating proteins).There are 14 GEFs and 24 GAPs ... EMBO J. 24,2244–2253. 10 Peter, B. J., Kent, H. M., Mills, I. -
Addit05_NeXtal pH gradient
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-05.pdf20 Nov 2020: 1 MD4 22 6.4 Sodium Cacodylate 1 MD5 23 6.6 Sodium Cacodylate 1 MD6 24 6.8 Sodium Cacodylate 1 ME1 25 7 Sodium HEPES 1 ME2 26 -
Structure of a Bacterial Dynamin-like Protein Lipid Tube Provides a…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cell%202009%20dynamin.pdf19 Dec 2009: ell 139, 1342–1352, December 24, 2009 ª2009 Elsevier Inc. 1347. A B. ... at either end. ell 139, 1342–1352, December 24, 2009 ª2009 Elsevier Inc. -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB12.pdf2 Oct 2014: Section D: Conditions (16 -18) and (22 - 24) supplements the CSS-I with other PEGS mixed with KSCN. • ... 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. -
Structure of a cholinergic cell membraneNigel Unwina,1 Edited by ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS22.pdf4 Nov 2022: The broken yellow lines identify the well-documented ribbon of δ subunit-linked receptor dimers(14, 15, 18, 24), one of which is labeled in each leaflet to indicate the subunit ... Biophys. J. 92, 3960–3967 (2007). 24. H. W. Chang, E. Bock, Molecular -
Addit04b_AL-SEL-DP Formated
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-04b.pdf24 Nov 2020: FOS-CHOLINE-UNSAT-11-10 31 FC-U10-11 FCU110 6.2 335.4B 12 24 1,2-Diheptanoyl-sn-glycero-3-phosphocholine 8.4 DHPC D607 1.4 481.5C ... OHES Bachem - P1105 24.3 n/aG 2 74 CYMAL-3 120 CYMAL-3 C323 30 466.5G 3 75 CYMAL-4 38 CYMAL-4 C324 7.6 480.5G 4 -
S413_6a 39..44
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreB%20Nature.pdf30 Aug 2001: 51.1 Å. IA. IBIIB. IIA. F-actin MreB. 55 Å. 1. 24. ... Interestingly, the rened model ofF-actin23,24 is less similar to our crystal structure than the initialunrened model, in which the crystal structure of G-actin incomplex with DNase I was -
1 Clear StrategyTM Screen I MD1-14 A 6 × ...
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB10.pdf2 Oct 2014: MD1-14 is presented as a 24 x 10† mL stock conditions 2 x 10 mL of 5 different pH’d buffers. ... 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: Genetics 159, 133–145 (2001). 24. Gaffen, S. L. Recent advances in the IL-17 cytokine family. ... Optogenetics. L4 animals expressing ChR2 in RMG27 were picked 24 h before the assay and divided into two groups. -
MD1-98 The LMB Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB21.pdf15 Jul 2016: Tris propane 7.11-22 24 % v/v Ethanol 0.1 M HEPES 7.8 0.04 M Magnesium chloride hexahydrate1-23 17.5 % v/v Ethanol1-24 1.3 M ... M Sodium chloride1-43 15 % w/v PEG 3350 0.1 M MES 6.21-44 24 % w/v PEG 3350 0.05 M HEPES 6.8 0.15 M Sodium -
Phosphoinositide-Specific Phospholipase Cδ1 Activity toward Micellar…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9287165.pdf20 Apr 2002: diC7PC (8 mM) no significant activation observed 0.85( 0.06 24.7( 2.9(1.7( 0.2). ... 132) PI-PLCδ1 and at 24-45 C for full-length PI-PLCδ1 (Figure 7A,B). -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB11.pdf2 Oct 2014: 24 0.8 M Na formate 8% PEG 20,000 8% PEG 500 MME. ... Section D: Conditions (16 -18) and (22 - 24) supplements the CSS-I with other PEGS mixed with KSCN. • -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-03.pdf9 Jul 2018: D3 21 1.5 % Caffeic acid †D4 22 1.5 % D-()-Quinic acidD5 23 1.5 % Gallic acid monohydrate†D6 24 1.5 % N-vanillylnonanamide (capsaicin synthetic)†E1 25 1.5 % -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/sedequil.pdf16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at -
Influence of lipid bilayer on the structure of the muscle-type…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf30 Apr 2024: in cell- derived membrane vesicles (24), or by in situ cryoelectron tomography (25). ... Electron Microscopy Data Bank https://www.ebi.ac.uk/emdb/EMD-18845. Deposited 6 November 2023. 24. N.Unwin, Influence of lipid bilayer on structure of acetylcholine
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