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Atomic advance for cryo-EM - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/atomic-advance-for-cryo-em/21 Oct 2020: Apoferritin is commonly used as a benchmark for cryo-EM, because its molecular stability and 24-fold symmetry allow high-resolution reconstructions from relatively few particles. -
A cellular pacemaker in the central body clock - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/a-cellular-pacemaker-in-the-central-body-clock/7 Jul 2020: Our behaviour, and the functions of our cells, follow a daily cycle in line with the 24-hour day. -
César Milstein Lecture to be given by Elaine Fuchs - MRC Laboratory…
https://www2.mrc-lmb.cam.ac.uk/cesar-milstein-lecture-to-be-given-by-elaine-fuchs/21 Jan 2020: He died in Cambridge on 24 March 2002. Further references:. Primary Sidebar. -
Addit05_NeXtal pH gradient
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-05.pdf20 Nov 2020: 1 MD4 22 6.4 Sodium Cacodylate 1 MD5 23 6.6 Sodium Cacodylate 1 MD6 24 6.8 Sodium Cacodylate 1 ME1 25 7 Sodium HEPES 1 ME2 26 -
Science Magazine dives into the science behind affordable cryo-EM -…
https://www2.mrc-lmb.cam.ac.uk/science-magazine-dives-into-the-science-behind-affordable-cryo-em/24 Jan 2020: 24 January, 2020. In long feature format, Science Magazine profiles structural biology scientists at the LMB, including Chris Russo and Nobel Prize winner Richard Henderson, and their work on bringing -
Publications
https://www2.mrc-lmb.cam.ac.uk/groups/jes/publications.html17 Sep 2020: Histone H3.3 is required to maintain replication fork progression after UV damage Current Biology 24, 2195-201. -
A connection between stress detection and lifestyle decisions in…
https://www2.mrc-lmb.cam.ac.uk/a-connection-between-stress-detection-and-lifestyle-decisions-in-worms/24 Nov 2020: 24 November, 2020. Green/yellow depicts the activation of XBP-1s in the nuclei of a starved worm, with red indicating the neurons that make tyramine. -
A map to show the way to sense smell, temperature, and humidity - MRC …
https://www2.mrc-lmb.cam.ac.uk/a-map-to-show-the-way-to-sense-smell-temperature-and-humidity/23 Jul 2020: the body clock in line with the 24-hour day. -
Putting science skills to new use during the pandemic - MRC…
https://www2.mrc-lmb.cam.ac.uk/putting-science-skills-to-new-use-during-the-pandemic/17 Jul 2020: In the final set-up we operated 24/7 in 12h shifts, from 8am-8pm and from 8pm-8am, something that took a while to get used to. -
Impress
https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf28 Sep 2020: Aids). 23 / 50. Screenshots. 24 / 50. Generic Objects. 25 / 50. ... Slide 24. Slide 25. Slide 26. Slide 27. Slide 28. Slide 29. -
A splice of life - Chemistry World looks at how structural biology…
https://www2.mrc-lmb.cam.ac.uk/a-splice-of-life-chemistry-world-looks-at-how-structural-biology-has-revealed-the-secrets-of-the-spliceosome/24 Mar 2020: Published on. 24 March, 2020. Clare Sansom describes the history of spliceosome research and its importance, focusing on the work of Kiyoshi Nagai and his group. -
Cryo-EM Model-Buildingwith Modern Coot Paul Emsley@ Ben Gurion…
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-2020-nov-slac.pdf10 Nov 2020: Slide 23. Slide 24. Slide 25. Slide 26. Slide 27. Slide 28. -
Addit04b_AL-SEL-DP Formated
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-04b.pdf24 Nov 2020: FOS-CHOLINE-UNSAT-11-10 31 FC-U10-11 FCU110 6.2 335.4B 12 24 1,2-Diheptanoyl-sn-glycero-3-phosphocholine 8.4 DHPC D607 1.4 481.5C ... OHES Bachem - P1105 24.3 n/aG 2 74 CYMAL-3 120 CYMAL-3 C323 30 466.5G 3 75 CYMAL-4 38 CYMAL-4 C324 7.6 480.5G 4 -
Sheet1
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit05_NeXtal%20pH%20gradient.xlsx8 Oct 2020: M. D6. 24. 6.8. Sodium Cacodylate. 1. M. E1. 25. 7. -
EMBO Practical Course on the Crystallization of Macromolecular…
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/cv/2020_01_Carter_CV.pdf31 Jan 2020: Nat Cell Biol. 18: 1018-24. 11) Schmidt H, Carter AP‡ (2016) Review: Structure and mechanism of the dynein. ... Science. 322: 1691-5. 24) Gennerich A, Carter AP, Reck-Peterson SL, Vale RD‡ (2007) Force-induced bidirectional stepping of cytoplasmic -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit-03.pdf13 Oct 2020: D3 21 1.5 % Caffeic acid †D4 22 1.5 % D-()-Quinic acidD5 23 1.5 % Gallic acid monohydrate†D6 24 1.5 % N-vanillylnonanamide (capsaicin synthetic)†E1 25 1.5 % -
Sheet1
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit04b_AL-SEL-DP_Formatted.xlsx24 Nov 2020: 31. FC-U10-11. FCU110. 6.2. 335.4. B. 12. 24. 1,2-Diheptanoyl-sn-glycero-3-phosphocholine. 8.4. DHPC. ... 24.3. n/a. G. 2. 74. CYMAL-3. 120. CYMAL-3. C323. 30. 466.5. -
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/pdb6f9n.ent
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/pdb6f9n.ent9 Nov 2020: 3 23 2.7722 - 2.7314 0.99 4106 214 0.3164 0.4048 REMARK 3 24 2.7314 - 2.6929 0.99 4005 246 0.3080 0.3705 REMARK 3 ... 24.2530 -42.4652 REMARK 3 T TENSOR REMARK 3 T11: 0.5957 T22: 1.1171 REMARK 3 T33: 0.4861 T12: -0.2186 REMARK 3 T13: -0.0172 T23: -
MD1-116-117 Morpheus III-Morpheus III HT-96
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB22.pdf13 Oct 2020: 3 8.5 30 % Precipitant Mix 21-23 B11 1.5 % Vitamins mix 0.1 M Buffer System 3 8.5 30 % Precipitant Mix 31-24 B12 1.5 % Vitamins mix ... 8.5 30 % Precipitant Mix 32-24 F12 0.6 % Antibiotics mix 0.1 M Buffer System 3 8.5 37.5 % Precipitant Mix 42-25 G1 1.2 -
Clockology Survey Thank you for your interest in participating ...
https://www2.mrc-lmb.cam.ac.uk/groups/oneill/wp-content/uploads/sites/24/Clockology-Survey-FINAL_14_07_2020_COMBINED.docx14 Jul 2020: Nycthemeral: Biological process or activity having the duration of 24 h or one day. ... A functional biological entity capable of self-sustaining oscillations with a period length of around 24 hours. -
The structure of human thyroglobulin
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf31 Jan 2020: Replacing all acceptor. tyrosines (Y) with phenylalanines (F) prevents hormone formation (4 acceptor residues: 24, 2573, 2766 and 1310). ... Eur. J. Endocrinol. 138, 227–231 (1998). 24. Dunn, J. T., Kim, P. -
FtsK in motion reveals its mechanism for double-stranded DNA…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK_Jean_2020_PNAS.pdf8 Jun 2020: This arrangementis reminiscent of the single spiral staircases observed in ring-shaped helicases (24). ... orienting the FtsK translocase. EMBO J. 24, 3770–3780 (2005).10. S. Bigot, O. -
Identifying proteins bound to native mitotic ESC chromosomes reveals…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf11 Aug 2020: 23.4 4.1, 43.6 6.9 µm2 respectively) or Eed/ (24.3 4.5,42.2 4.7 µm2, respectively) ESCs are significantly larger thanequivalent chromosomes isolated from WT ... 24 %. 70 %. async m-arrested. coun. t. PI intensity. Cardiomyocytes. -
16001658465475 1..36
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Collier_2020_elife-59560-v1.pdf15 Sep 2020: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 33. Received: -
An intramembrane chaperone complex facilitates membrane protein…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/Nature_2020.pdf17 Aug 2020: Cell 170, 298–311.e20 (2017). 24. Stein, K. C., Kriel, A. & Frydman, J. ... After 24 h, transfected cells were selected for 48 h with 1 μg ml1 puromycin then trypsinized and diluted into 96-well plates at a concentration of 0.5 cells per -
15919734904046 1..30
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020.pdf12 Jun 2020: α-FLAG. Nat. IPα-FLAG. α-EMC1. EMC1. 1 x 3. Lysate. – – –. 23 24 25 26 23 23. C. EMC3. amb:. detergentmicelle. BBBBBBBBBBB. -
15955888524538 1..29
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020b.pdf24 Jul 2020: transgenes. was allowed for 24 hr. After additional 20 hr of doxycycline-induced expression of the stalling reporter, GFP expression was analysed by flow cytometry.
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