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Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&2 May 2013: Acta Crystallogr. D Biol. Crystallogr. 60, 203–204 (2004). Solubility Screen. Protein characteriza0on 1-‐10mg/ml 100nl/drop Incubate 1-‐24 hrs. ... Or Jena Bioscience JBScreen Solubility HTS. Solubility Screen. Protein characteriza0on 1-‐10mg/ml -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/molecular-replacement/?wpdmdl=18799&11 Jun 2013: 24 (12) RF/TF. 3 (1) Pack. 3 (1) Refined. 307 (283) RF/TF. ... 68 (64) Pack. 24 (2) Refined. 32 (20) RF/TF. 22 (17) Pack. -
Slide 1
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/processing.pdf13 May 2013: approach. 24/44. 24/44. Integration by profile fitting. Based on the assumption that spots corresponding to fully recorded reflections in the same region of the detector (and on images nearby -
Cooperative Recruitment of Dynamin and BIN/Amphiphysin/Rvs (BAR)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/JBC_Meinecke2013.pdf19 Mar 2013: nexin 9 (SNX9) (23, 24). It has a BAR and an SH3 domain thatbinds to dynamin.The involvement of BAR domain proteins in dynamin activ-. ... J. Biol. Chem. 278, 46772–46781. 24. Soulet, F., Yarar, D., Leonard, M., and Schmid, S. -
Kevin Cowtan, cowtan@ysbl.york.ac.uk 2012 Automated phase…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/densitymod12-ppt.pdf28 May 2013: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. -
, 130081, published 7 August 20133 2013 Open Biol. ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Open%20Biol.-2013-TakedaCombined.pdf25 Sep 2013: compacted by the ingressingcleavage furrow (figure 2e, wild-type, 16 – 24 min; electronicsupplementary material, movie S3). ... GFP alone, the cleavage furrowstarted to ingress within 4 min after anaphase onset at2.24 mm min – 1 (s.e.m. -
pone.0052401 1..15
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Podosome2012/journal.pone.0052401.pdf10 Jan 2013: clear amphipathic characteristics and fold on membrane binding. [4]. A 24 and 28 amino acid long helix, H0, would be possible for. ... Members of the PAK family are major. effectors of the Rho GTPases CDC42 and Rac (reviewed in [24]). -
Qrb1300006 1..40
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf30 Jul 2013: Transition to open-channel form 23. 5.1. Introduction 23. 5.2. Spray-freeze-trapping experiments 24. -
Deubiquitinases Sharpen Substrate Discrimination during Membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/82_Zhang_ZR_Cell_2013.pdf5 Aug 2013: panel) plasmids were cotransfected with a fixed. amount (100 ng) of CD4 and analyzed 24 hr later by. -
Slide 1
https://www2.mrc-lmb.cam.ac.uk/download/integration-and-scaling-harry-powell-mrc-lmb-crystallography-course-10th-may-2013/?wpdmdl=18796&refresh=669147146af7d172079694813 May 2013: approach. 24/44. 24/44. Integration by profile fitting. Based on the assumption that spots corresponding to fully recorded reflections in the same region of the detector (and on images nearby
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