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24 Sep 2004: Protein atoms 4404 4164 4138Waters 265 324 259Rcrystd 20.2 19.9 22.6Rfreed (% data used) 24.1 (5) 24.3 (5) 26.7 (5)r.m.s.d. ... inosi-in 1 l sitting drops over reservoirs of 24-well plates (Hampton).

21 Dec 2004: Between 5000 and 10,000 cells were plated per well of a 96-well platebetween 24 and 36 h before transfection, at which time they were 40 – 80%confluent. ... Unless other-wise noted in the figure legend, the cells were analyzed 18 –24 h after

1 Nov 2004: Multiplicity (last shell) 14.1 (14.1) 14.3 (14.2) 11.6 (11.6) 7.2 (7.2) 6.9 (3.9) 24.9 (24.0)I/ (last shell)

23 Nov 2004: Transferrin uptake and immunofluorescenceCos 7 cells were transfected using GeneJuice according to manufacturers instructions,plated onto polylysine coated coverslips 24 hours post transfection and assayed fortransferrin uptake 48 hours post

30 Jan 2004: recruit clathrin to membranes. Mammalian am-phiphysin2 has several clathrin-binding sites (24–26), and we found that the wild-type, but notmut1, efficiently recruited clathrin to liposomes(fig. ... It is interesting to note that thestructure of the

23 Nov 2004: Curr. Opin.Cell Biol. 14, 454–462. 24. Seaman, M.N.J., Marcusson, E.G., Cereghino, J.L., and Emr,S.D.

2 Feb 2004: Membrane transport between compartments in eukary-otic cells requires proteins that allow the budding andSCISSION of nascent cargo vesicles from one compartmentand their targeting and fusion with another. Just asSNARES are proposed to be essential

3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24.

29 Dec 2004: rg/ ml for 24 h at 37 “C).

6 Dec 2004: Received for publication, July 20, 2004, and in revised form, August 24, 2004Published, JBC Papers in Press, August 25, 2004, DOI 10.1074/jbc.M408179200. ... MIN6 Cell Culture—MIN6 -cells (24) were used between passages19 and 30 and grown in