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MR_LMB
https://www2.mrc-lmb.cam.ac.uk/download/molecular-replacement/?wpdmdl=18799&refresh=6691471425490172079694811 Jun 2013: 24 (12) RF/TF. 3 (1) Pack. 3 (1) Refined. 307 (283) RF/TF. ... 68 (64) Pack. 24 (2) Refined. 32 (20) RF/TF. 22 (17) Pack. -
Structure and superorganization of acetylcholinereceptor–rapsyn…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf3 Jul 2013: 20%of the receptors showed restricted diffusion in small domains of50 nm, whereas the majority of the receptors were immobile (24).Confocal microscopy of overexpressed rapsyn in nonmuscle. ... The cross-linking of receptors by rapsyn,therefore, seems to -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/MR_LMB.pdf11 Jun 2013: 24 (12) RF/TF. 3 (1) Pack. 3 (1) Refined. 307 (283) RF/TF. ... 68 (64) Pack. 24 (2) Refined. 32 (20) RF/TF. 22 (17) Pack. -
The current approach to initial crystallization screening of proteins …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gorrec_undersamplid_2013.pdf14 May 2013: Appl. Cryst. 24, 409–411.Kimber, M. S., Vallee, F., Houston, S., Necakov, A., Skarina, T., Evdokimova,. -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/20130430_Cryst_course.pdf2 May 2013: Acta Crystallogr. D Biol. Crystallogr. 60, 203–204 (2004). Solubility Screen. Protein characteriza0on 1-‐10mg/ml 100nl/drop Incubate 1-‐24 hrs. ... Or Jena Bioscience JBScreen Solubility HTS. Solubility Screen. Protein characteriza0on 1-‐10mg/ml -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: coli FtsKN used in the in vivo experiments, 24 amino acids forthe E. ... Mol. Microbiol.27:257–268. 24. Steiner W, Liu G, Donachie WD, Kuempel P. -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&refresh=66914714c123217207969482 May 2013: Acta Crystallogr. D Biol. Crystallogr. 60, 203–204 (2004). Solubility Screen. Protein characteriza0on 1-‐10mg/ml 100nl/drop Incubate 1-‐24 hrs. ... Or Jena Bioscience JBScreen Solubility HTS. Solubility Screen. Protein characteriza0on 1-‐10mg/ml -
pnas201313978 4601..4610
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas2013duman.pdf23 Dec 2013: AnezrA-null mutant forms slightly longer cells (20%) comparedwith wild-type cells (23), but an ftsA null mutant has problemsdividing and forms long filamentous cells (24). -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&refresh=669147153938d172079694911 Jun 2013: 04/06/2013 Crystallography Course LMB 24. (Pseudo)merohedral twining is impossible. 432 622. ... Data processing ( -‐1 ). Molecular replacement ( P1 ). Refinement ( P1 ). R / R-‐free = 0.24 / 0.31. PDB: 1yup ( P1 ). PDB: 1yup. Zanuda. ( P21 ) -
Kevin Cowtan, cowtan@ysbl.york.ac.uk 2012 Automated phase…
https://www2.mrc-lmb.cam.ac.uk/download/automated-phase-improvement-and-model-building-kevin-cowtan-cowtanysbl-york-ac-uk/?wpdmdl=18786&refresh=6691471564838172079694928 May 2013: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24.
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