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5 Dec 2012: 20 nM USP21i86. 24. 0 0.2 0.4 0.6 0.8 1.0FRET efficiency. ... 100 nM USP21i86. 24. 0 0.2 0.4 0.6 0.8 1.0FRET efficiency.

1 Aug 2008: 2008). Biol. Cell, in press. Pub-lished online January 24, 2008. 10.1042/BC20070149.

16 Sep 2003: Y Y. 24 25 26. Q K. 27. A L. 28 29 30. ... A G. 24 25 26. G A. 27. D V. 28 29 30.

23 Aug 2004: plot are linear [24]. In comparison, deviation from linearity. blished by Elsevier B.V. ... 0.235. 0.24. 0 0.5 1 1.5 2. Flu. ores. cenc. e.

11 Sep 2003: Methods and Applications,ActaCrystallogr. D49, 24-36. 6. Buckle, A. M., Henrick, K., and Fersht, A. ... 20. Kraulis, P. (1991)J. Appl. Crystallogr. 24, 946-950.21. Laitinen, P. J., Brown, K.

26 Aug 2005: 4ANK TALR ANK 4 1.8 12.3 – – [27]. E2_5 ANK 4 3.3 11.4 79 – [24,25]. ... be easily lengthened or shortened by the addition orremoval of individual repeat motifs [12,15,16,22,23,24,25].

16 Sep 2003: email: sej13@cam.ac.uk. Received 6 January, 1999; accepted 24 May, 1999. 1. ... Prot. Chem. 24, 1–95 (1970).6. Chiti, F. et al. J. Mol.

15 Sep 2003: L.; Rose, G. D.Trends Biochem. Sci. 1999, 24, 77-83.(60) Baldwin, R. ... L.; Rose, G. D.Trends Biochem. Sci. 1999, 24, 26-33.(61) Fersht, A.

16 Sep 2003: Received 19 November 2002; accepted 19 December 2002. The first international meeting on the Hsp90 (heat-shock protein 90)chaperone machine was held in Arolla, Switzerland, from 24 to 28 August

10 May 2010: ture, or that knotting may even occur in the denatured ensemble(24, 25). ... relative ease with which they can form their nativestates during in vitro folding experiments (24, 26, 27).

17 Sep 2003: This increasedto 0.81 (n ) 24) on inclusion of mutations of partially buried hydrophobic residues. ... 23. Shortle, D., and Meeker, A. K. (1989)Biochemistry 28, 936-944. 24.

15 Sep 2003: The IC50 is approximately 10 mM (data notshown) similar to that measured for yeast Hsp90.24. ... J. Biol. Chem. 274, 17525-17533. 24. Panaretou, B., Prodromou, C., Roe, S.

8 Dec 2008: J Mol Biol346:1409 –1421. 24. Tanford C (1968) Protein denaturation. Part A. ... Theoretical models for the mechanismof denaturation. Adv Protein Chem 24:1–95. 26.

8 Feb 2008: with a Kd of 29(3) nM, following incubationfor 24 h (Figure 2(d)). ... Kd (nM)4 h. Kd (nM)8 h. Kd (nM)24 h. kon(μM1 s1) koff.

6 Jan 2005: Mol. Biol. Cell 12, 3307–3316. (2001).24. Hossain, M.M. & Nakamoto, H.

17 Jul 2005: first 24 h, then the number of scans wasdoubled, and data were collected over the next 5– 40 days. ... 1 T kin† T eq.‡. CTPR2 19,860 6,692 50 2.24 0.19 0.35 0.21 2.06 0.18 0.5 0.4CTPR3 35,032 7,601

17 Jul 2005: J. Mol. Biol. 307, 17–24. Ceruso, M. A., Amadei, A. & Di Nola, A.

23 Aug 2004: Wild-type and mutant proteins wereexpressed and purified as described elsewhere (24-25). ... Struct. Biol. 8, 331-333. 24. Finucane, M. D., Tuna, M., Lees, J.

8 Feb 2008: caused by aggregation of the native state, WT⁎ at 2 μM, pH 7.4and 37 C was equilibrated for 24 h in 300 g/L glucose. ... Side-. chain transfer is not, however, sufficient to overcome theunfavourable transfer of the backbone, as observed with

13 Sep 2006: J. Mol. Biol. 307, 17–24. Ceruso, M. A., Amadei, A. & Di Nola, A.

16 Nov 2006: In contrast to the above mechanisms, one computational DFTstudy suggested that oxidation could precede cyclization [24]. ... 14 hours 24 hours 48 hours 1 week2 weeks3 weeks 4 weeks 5 weeks 6 weeks 7 weeks 8 weeks.

11 Sep 2003: given here is twice that of the monomer [23]. mInitial reports suggested that wild-type ubiquitin folded with two-state kinetics [24]. ... 4]), activation domain procarboxypeptidase (ADAh2; [22]), ubiquitin[24,25], histidine-containing phosphocarrier

16 Sep 2003: In addition, we have shown that TFE does not significantly change the fold-ing pathway of FKBP12 [24]. ... However, although TFE affects the folding andunfolding rate of FKBP12, we have established that this effect is not caused bythe stabilisation of a

11 Feb 2008: W.; Fesinmeyer, R. M.; Andersen, N. H.Nature Struct. Biol. 2002, 9, 425-430.(24) Frisch, M. ... 2 ) 1.26 1020, HFC2 ) 6.24 1018, andXG,HFC) -4.26 1018.

26 Jul 2010: AttoOxa11 dyes,24,25 or the use of highly fluorescent. proteins such as yellow fluorescent protein (YFP).26. ... Biotechnol Prog23:1506–1512. 24. Hoffmann A, Kane A, Nettels D, Hertzog DE, Baum-gartel P, Lengefeld J, Reichardt G, Horsley DA, SecklerR,

4 Jun 2008: 5b), confirming that Cyp40 insolution exists in this state23,24, suggesting that theobserved structure of the tetragonal crystal form islikely to be a crystallographic artifact. ... Structure, 9, 431–438. 24. Ward, B. K., Allan, R. K., Mok, D., Temple,

5 Jun 2006: groups have also studied the effect of mutation of hydrophobiccore groups on the stability.22–24 In these cases, mutation of buriedhydrophobic core residues destabilises the native state of ubiquitinwith the ... Acad. Sci. U. S. A., 2004, 101,

3 Apr 2009: disease [22–24]. No stability or folding studies on any of the 52-knot-. ... 24 Setsuie R & Wada K (2007) The functions of UCH-L1.

14 Nov 2011: There is a general view that Hop/FKBP52 may competefor available binding sites in the Hsp90 dimer (13, 16, 23, 24).The lack of reliable dissociation constants means that it is ... J Biol Chem 273:18007–18010. 24. Radanyi C, Chambraud B, Baulieu EE (1994

8 Feb 2008: Structuresfor the isolated, individual domains have beenreported either for human or yeast Hsp90, the ERhomologue Grp94 or the Escherichia coli homologue,HtpG.18–24 In addition, a number of structures oflarger ... Trends Biochem. Sci. 24, 136–141. 4.

17 Sep 2003: Sci.24: 136–141. Chen, S., Prapapanich, V., Rimerman, R.A., Honore, B., and Smith, D.F.

1 Aug 2008: AM. CHEM. SOC. 2008, 130, 7898–7907. identified as a continuum-of-substates,19 expansion or collapseof the unfolded state,18,23,24 or just assigned to changes in thedye’s ... Proc. Natl. Acad. Sci. U.S.A. 2006, 103, 11539–11543. (24) Hoffmann, A.;

17 Jul 2005: This observation is notexpected for a simple two-state denaturation mech-anism, and indicates that a more complex scheme isnecessary to describe the system fully.24,25 In particu-lar, the ... transition is biphasic33–37 and each transition can

19 Dec 2008: Neuberger, A (ed.), pp 23–24,Methuen, London. Mayo, SL, and Baldwin, RL (1993).

23 Aug 2004: Received 24 March 2004; received in revised form 14 May 2004; accepted 17 May 2004. ... 24] within the GROMACS software package. This. method divides the conformational space of the protein.

15 Sep 2003: Ala75 2.6 0.11 ÿ5.51 0.12 1.01 0.02 ÿ1.55 0.04 0.34 0.03 0.24 0.01Thr! ... Val76 0.76 0.12 ÿ7.90 0.12 0.94 0.02 ÿ4.24 0.05 0.56 0.2 0.43 0.09Ile!

8 Feb 2008: Sextuple 2.6 ( 0.4) 3 102 2.0 ( 0.6) 3 103 0.24 0.07 0.33 0.03 0.6 0.1 1.5 0.3.

13 Jan 2012: coli 0 4.7 0.1 2.9 0.1 21.8 0.5 –. 1.0 5.5 0.1 24.1 0.6 21 9In vitro expression in a ... pulse proteolysis and western blotting. Protein Sci. 18, 1051–1059 (2009).24. Park, C. &

19 Apr 2005: Forexample, 13 of the proteins characterized here were in-cluded in an earlier 24-protein data set that was used toillustrate the relationship between an empirical measure oftopology termed relative contact ... 07 2.04 0.03 13.0 1.9 4.2 0.5

6 Jan 2005: Trends Biochem. Sci. 25,24–28. 17. Weikl, T., Muschler, P., Richter, K., Veit, T., Reinstein, J.& Buchner, J. ... J. Biol. Chem. 275, 37181–37186. 24. Adair, G. S. (1925). The hemoglobin system.

15 Sep 2003: Article No. jmbi.1999.2941 available online at http://www.idealibrary.com on J. Mol. Biol. (1999) 291, 429444. Folding Pathway of FKBP12 and Characterisation ofthe Transition State. Ewan R. G. Main, Kate F. Fulton and Sophie E. Jackson. Cambridge

8 Feb 2008: 19–24 as well as on cooperatively unfoldingregions of proteins.11,14 Although H/D exchangeresults have been reported for GFP, the conditionsused did not allow a quantitative analysis of theresults ... Thesymbols are the same as for (a) except the first

8 Feb 2010: pH 8.0 37 CYF 2.22 0.06 5.10 0.01 11.3 0.3 5.11 0.02 8.74 0.04Tyr 1.51 0.07 5.24

25 Aug 2006: W.Prog. Nucl. Magn. Reson. Spectrosc.1993,. 25, 345-402.(24) Kaptein, R.Bio. Magn. Reson.1982, 4, 145-91.(25) Kaptein, R.; Dijkstra, K.; Nicolay, K.Nature 1978, 274,

6 Sep 2005: 0.04 4.46 6 0.07 0.50 6 0.03 1.24 6 0.01 4.22 6 0.07 2.74 6 0.07Ile!Val23 0.45 6 ... 0.04 5.54 6 0.10 0.57 6 0.04 1.24 6 0.02 5.18 6 0.08 2.74 6 0.07Ile!Ala23 2.78 6

4 Jul 2006: Double-jump unfolding experiments wereused to detect faster unfolding phases corres-ponding to non-native species populated on therefolding pathway.24–26 In these experiments,refolding was allowed for short amounts ... 0.940.10 0.250.02 1.20.1

28 Sep 2009: pH 6.0 at 25 1C as a function of equilibration time: from right to left 3 h, 12 h, 24 h, 48 h, 5 days, 13 days, 44 days (adapted ... group to demonstrate how information on protein structure. can be obtained using a mechanical triangulation technique.24.

21 Apr 2006: p23appears to stabilise the interaction between Hsp90and a client protein in vitro,23,24 correlating with itsability to increase the activation of heterologouslyexpressed estrogen receptor in vivo.25 In contrast,another

23 Aug 2004: However, it stronglyinhibits the ATPase activity in the presence of aclient-protein.10 Recent work has shown that Sti1/Hop binds to the first 24 amino acid residues ofHsp90 thereby preventing ... N terminus ofHsp90 is within the first 24 amino acid

8 Feb 2008: topology that led to the hypothesisthat topology, not sequence, is the major contribut-ing factor in how a protein folds.23,24 If proteinfolding rates and mechanisms are determinedlargely by the