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  2. 14 May 2024: Search site. Research Facilities at the Department of Biochemistry. Biophysics. Research Facilities at the Department of Biochemistry. Biophysics. Welcome to the Biophysics Facility at the Department of Biochemistry, University of Cambridge. We are
  3. ITC_sample_preparation_310713

    https://facilities.bioc.cam.ac.uk/files/media/itc_sample_preparation_310713.pdf
    31 Jan 2021: amino acid analysis assay. Errors in the cell concentration affect the stoichiometry (n); errors in the syringe concentration are more problematic as they affect n, Ka and ΔH. ... it competes for binding, to correct Kd, measure Kd of protein DMSO in a
  4. ITC_sample_preparation_310713

    https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/itc_sample_preparation_310713.pdf
    31 Jan 2021: amino acid analysis assay. Errors in the cell concentration affect the stoichiometry (n); errors in the syringe concentration are more problematic as they affect n, Ka and ΔH. ... it competes for binding, to correct Kd, measure Kd of protein DMSO in a
  5. Fluorescence Anisotropy Theory Method and Data Analysis

    https://facilities.bioc.cam.ac.uk/files/media/fluorescence_anisotropy_theory_method_and_data_analysis.pdf
    17 Nov 2023: 2&'IJ𝑃𝐿 Where kon is the forward rate constant. The equilibrium association constant, Ka, for this reaction is described by the following expression: Eq 3.2 𝐾3 =. ['5]['][5]. The dissociation ... 5]%8)['][5])= 8)['][5]. (,%8)['])[5]= 8)[']. ,%8)[
  6. Table Of Contents

    https://facilities.bioc.cam.ac.uk/files/media/itc200_user_manual_rev_d.pdf
    31 Jan 2021: Origin software is then used to analyze the ITC data using fitting models to calculate reaction stoichiometry (n), binding constant (Ka), enthalpy (ΔH) and entropy (ΔS). ... A pair of options at the bottom of this tab allows users to work in Kd or Ka,
  7. Fluorescence Anisotropy Theory Method and Data Analysis

    https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/fluorescence_anisotropy_theory_method_and_data_analysis.pdf
    17 Nov 2023: 2&'IJ𝑃𝐿 Where kon is the forward rate constant. The equilibrium association constant, Ka, for this reaction is described by the following expression: Eq 3.2 𝐾3 =. ['5]['][5]. The dissociation ... 5]%8)['][5])= 8)['][5]. (,%8)['])[5]= 8)[']. ,%8)[
  8. MAU130010 Rev D ITC Data Analysis in Origin Tutorial Guide–

    https://facilities.bioc.cam.ac.uk/files/media/vp_itc_fitting_manual.pdf
    31 Jan 2021: MAU130010 Rev D. MicroCal, LLC 22 Industrial Drive East. Northampton, MA 01060 USA Phone: 413 586 7720. Fax: 413 586 0149 Binding Stability Kinetics support@microcalorimetry.com. ITC Data Analysis in Origin. Tutorial Guide Version 7.0 - January
  9. Table Of Contents

    https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/itc200_user_manual_rev_d.pdf
    31 Jan 2021: Origin software is then used to analyze the ITC data using fitting models to calculate reaction stoichiometry (n), binding constant (Ka), enthalpy (ΔH) and entropy (ΔS). ... A pair of options at the bottom of this tab allows users to work in Kd or Ka,
  10. MAU130010 Rev D ITC Data Analysis in Origin Tutorial Guide–

    https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/vp_itc_fitting_manual.pdf
    31 Jan 2021: MAU130010 Rev D. MicroCal, LLC 22 Industrial Drive East. Northampton, MA 01060 USA Phone: 413 586 7720. Fax: 413 586 0149 Binding Stability Kinetics support@microcalorimetry.com. ITC Data Analysis in Origin. Tutorial Guide Version 7.0 - January

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