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Dlp Purification:
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Eva%20Methods.htm6 Feb 2008: Affinities were calculated via fitting titration curves to the data. This resulted in the stoichiometry N, the binary equilibrium constant Ka, the dissociation constant Kd (which equals 1/Ka), the enthalpy -
16001658465475 1..36
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Collier_2020_elife-59560-v1.pdf15 Sep 2020: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 33. Received: -
https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/58
https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/58KR, Duchek P, Edwards OR, Esvelt K, Gantz VM, Golic KG, Gratz SJ, Harrison MM, Hayes KR, James AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O’Connor-Giles KM, -
Public Engagement - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/news-events/public-engagement/feed/19 Jul 2024: A thermostable, closed SARS-CoV-2 spike protein trimer./a Xiong, X., Qu, K., Ciazynska, KA., Hosmillo, M., Carter, AP., Ebrahimi, S., Ke, Z., Scheres, SHW., Bergamaschi, L., Grice, GL., Zhang, -
Low energy cryo-EM to widen accessibility for fast and accurate…
https://www2.mrc-lmb.cam.ac.uk/low-energy-cryo-em-to-widen-accessibility-for-fast-and-accurate-structure-determination/28 Nov 2023: McMullan, G., Naydenova, K., Mihaylov, D., Yamashita, K., Peet, MJ., Wilson, H., Dickerson, JL., Chen, S., Cannone, G., Lee, Y., Hutchings, KA., Gittins, O., Sobhy, MA., Wells, T., El-Gomati, MM., -
373_431_BIOsp_0411
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Biospektrum2011%20Gasper.pdf1 Jul 2011: Int JBiochem Cell Biol 41:323–329[3] Aylett CH, Wang Q, Michie KA et al. ... Cell 115:705–713[12] Michie KA, Löwe J (2006) Dynamic filaments of the bac-terial cytoskeleton. -
Contrasting Membrane Interaction Mechanisms of AP180N-terminal…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Stahelin_cho2003.pdf16 Jul 2003: smaller ka) but prolonged its membrane residence by3-fold (i.e. smaller kd). ... Proteins ka kd kdFold increase. in Kda. M 1 s 1 s 1 M. -
Electron Cryo-microscopy - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/news-events/public-engagement/cryo-electron-microscopy/feed/19 Jul 2024: A thermostable, closed SARS-CoV-2 spike protein trimer./a Xiong, X., Qu, K., Ciazynska, KA., Hosmillo, M., Carter, AP., Ebrahimi, S., Ke, Z., Scheres, SHW., Bergamaschi, L., Grice, GL., Zhang, -
cdf519 5057..5068
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12356722.pdf12 Oct 2002: The enhanced membrane afnity of the p47phox-PXdomain due to 3 mol% PtdIns(3,4)P2 is almost entirely dueto a decrease in the kd, with the ka remaining nearlyconstant. ... Phosphoinositide ka (M1 s1) kd (s. 1) Kd (M) Fold increase in Kda. -
pnas201205508 15259..15264
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22949682.pdf25 Nov 2012: Full lipid-binding curves used tocalculate Ka values are in Fig. S7. ... B) Protein–lipid FRET assays were per-formed with 5% PIP2 vesicles, and Ka was calculated for both 5 μM pY.
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