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Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&refresh=669a852400fdd17214026602 May 2013: Use PCR tubes if needed Thaw in hand, then store on ice Find op-mal storage buffer (use solubility screen). ... Kim, R. Op-mum solubility (OS) screening: an efficient method to op-mize buffer condi-ons for homogeneity and crystalliza-on of proteins. -
Structural analysis of the ParR/parC plasmidpartition complex Jakob…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MollerJensen_ParR_EMMBO_2007.pdf4 Oct 2007: nis. otr. op. y. ParR171 (µM). Generations. Pla. smid. co. nte. -
x96255u149
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11090628.pdf20 Apr 2002: Molecular Cell912. Fig. ure. 5.S. tere. op. lots. of. the. Act. -
The ER membrane protein complex is a transmembrane domain insertase
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017c.pdf27 Jan 2018: S17) and op-timized conditions for its reconstitution into lipo-somes. The reconstituted EMC remained fullyintact (fig. -
doi:10.1016/j.molcel.2004.08.004
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15350214.pdf24 Sep 2004: Analytical UltracentrifugationReferencesSedimentation equilibrium experiments were done in Beckman Op-. tima XL-I ultracentrifuge with a Ti-60 rotor using interference andBalla, T., Sim, S.S., Baukal, A.J., Rhee, S.G., -
doi:10.1016/j.str.2008.06.010
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/18786397.pdf3 Sep 2008: direction, toward the surface of the filament. The. molecules in the repeating unit one symmetry op-. -
Structural insight into the ESCRT-I/-II link and itsrole in ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17215868.pdf14 Jun 2007: decreases the affinity more than 60-fold) (Table II) as op-. posed to the weaker interaction formed by Vps36-NZF-C. -
Making pre+y pictures So how does one go about ...
https://www2.mrc-lmb.cam.ac.uk/download/14-making-pretty-pictures-of-macromolecules-meindert-lamers/?wpdmdl=18789&refresh=669a852509a00172140266111 Jun 2013: formulate ques-on very hard! make sample cloning, expression, purifica-on make crystal screening, op-misa-on collect diffrac-on data synchrotron, integra-on, scaling solve phase problem MR, SIRAS, MIRAS, ... The journal uses the RGB format to op-mize -
Protection from cytosolic prion protein toxicityby modulation of…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/30_Rane_N_EMBO_2004.pdf8 Nov 2004: Protection from cytosolic prion protein toxicityby modulation of protein translocation. Neena S Rane, Jesse L Yonkovich andRamanujan S Hegde. Cell Biology and Metabolism Branch, NICHD, National Institutesof Health, Bethesda, MD, USA. Failure to -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&2 May 2013: Use PCR tubes if needed Thaw in hand, then store on ice Find op-mal storage buffer (use solubility screen). ... Kim, R. Op-mum solubility (OS) screening: an efficient method to op-mize buffer condi-ons for homogeneity and crystalliza-on of proteins.
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