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  2. publications | The Bacterial Cytoskeleton and other Molecular Machines

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/page-4/index.html
    29 Apr 2024: Jan Löwe's group at the MRC Laboratory of Molecular Biology. Jan Löwe's ORCID identifier: 0000-0002-5218-6615. For statistics such as h-index and citation numbers please consult Google Scholar: Jan Löwe's profile. 2024. Hale V., Hooker J., Russo C
  3. Published Research - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/research/published-research/
    Thumbnail for Published Research - MRC Laboratory of Molecular Biology 21 Jul 2023: Nature 630(8017): 596-608. Prentice, A., Jarjou, LM., Goldberg, GR., Schoenbuchner, SM., Moore, SE., Ward, KA., Cole, TJ.
  4. https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/1018

    https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/1018
  5. Publications

    https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/publications/
    AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O’Connor-Giles KM, Parks AL, Perrimon N, Port F, Russell S, Ueda R, Wildonger J (2015) Biosafety: Safeguarding gene drive experiments
  6. https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/585452

    https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/585452
    {"id":585452,"date":"2023-11-28T10:54:06","date_gmt":"2023-11-28T10:54:06","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?p=585452"},"modified":"2023-12-11T12:23:41","modified_gmt":"2023-12-11T12:23:41","slug":"low-energy-cryo-em-to-widen-access
  7. full Publications

    https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/publications/full_publications/
    MM, Hayes KR, James AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O’Connor-Giles KM, Parks AL, Perrimon N, Port F, Russell S, Ueda R, Wildonger J (2015) Biosafety:
  8. https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/18964

    https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/18964
    {"id":18964,"date":"2020-08-18T14:05:27","date_gmt":"2020-08-18T13:05:27","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?p=18964"},"modified":"2022-03-21T15:56:30","modified_gmt":"2022-03-21T15:56:30","slug":"studying-the-spike-protein-of-sars-c
  9. 16001658465475 1..36

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Collier_2020_elife-59560-v1.pdf
    15 Sep 2020: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 33. Received:
  10. Studying the spike protein of SARS-CoV-2 - MRC Laboratory of…

    https://www2.mrc-lmb.cam.ac.uk/studying-the-spike-protein-of-sars-cov-2/
    Thumbnail for Studying the spike protein of SARS-CoV-2 - MRC Laboratory of Molecular Biology 18 Aug 2020: Xiong, X., Qu, K., Ciazynska, KA., Hosmillo, M., Carter, AP., Ebrahimi, S., Ke, Z., Scheres, SHW., Bergamaschi, L., Grice, GL., Zhang, Y., The CITIID-NIHR COVID-19 BioResource Collaboration, Nathan, JA.,
  11. https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/29

    https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/29
    KG, Gratz SJ, Harrison MM, Hayes KR, James AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O’Connor-Giles KM, Parks AL, Perrimon N, Port F, Russell S, Ueda R,
  12. Dlp Purification:

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Eva%20Methods.htm
    6 Feb 2008: Affinities were calculated via fitting titration curves to the data. This resulted in the stoichiometry N, the binary equilibrium constant Ka, the dissociation constant Kd (which equals 1/Ka), the enthalpy
  13. 373_431_BIOsp_0411

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Biospektrum2011%20Gasper.pdf
    1 Jul 2011: Int JBiochem Cell Biol 41:323–329[3] Aylett CH, Wang Q, Michie KA et al. ... Cell 115:705–713[12] Michie KA, Löwe J (2006) Dynamic filaments of the bac-terial cytoskeleton.
  14. Low energy cryo-EM to widen accessibility for fast and accurate…

    https://www2.mrc-lmb.cam.ac.uk/low-energy-cryo-em-to-widen-accessibility-for-fast-and-accurate-structure-determination/
    Thumbnail for Low energy cryo-EM to widen accessibility for fast and accurate structure determination - MRC Laboratory of Molecular Biology 28 Nov 2023: McMullan, G., Naydenova, K., Mihaylov, D., Yamashita, K., Peet, MJ., Wilson, H., Dickerson, JL., Chen, S., Cannone, G., Lee, Y., Hutchings, KA., Gittins, O., Sobhy, MA., Wells, T., El-Gomati, MM.,
  15. https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/58

    https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/58
    KR, Duchek P, Edwards OR, Esvelt K, Gantz VM, Golic KG, Gratz SJ, Harrison MM, Hayes KR, James AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O’Connor-Giles KM,
  16. Contrasting Membrane Interaction Mechanisms of AP180N-terminal…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Stahelin_cho2003.pdf
    16 Jul 2003: smaller ka) but prolonged its membrane residence by3-fold (i.e. smaller kd). ... Proteins ka kd kdFold increase. in Kda. M 1 s 1 s 1 M.
  17. Public Engagement - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/news-events/public-engagement/feed/
    2 Jul 2024: A thermostable, closed SARS-CoV-2 spike protein trimer./a Xiong, X., Qu, K., Ciazynska, KA., Hosmillo, M., Carter, AP., Ebrahimi, S., Ke, Z., Scheres, SHW., Bergamaschi, L., Grice, GL., Zhang,
  18. 2168 | K. von der Malsburg et al. Molecular ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf
    11 Aug 2015: 2168 | K. von der Malsburg et al. Molecular Biology of the Cell. MBoC | ARTICLE. The ribosome quality control pathway can access nascent polypeptides stalled at the Sec61 transloconKarina von der Malsburg, Sichen Shao, and Ramanujan S. HegdeMRC
  19. cdf519 5057..5068

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12356722.pdf
    12 Oct 2002: The enhanced membrane afnity of the p47phox-PXdomain due to 3 mol% PtdIns(3,4)P2 is almost entirely dueto a decrease in the kd, with the ka remaining nearlyconstant. ... Phosphoinositide ka (M1 s1) kd (s. 1) Kd (M) Fold increase in Kda.
  20. pnas201014665 19609..19610

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Nogales-19609-10.pdf
    11 Nov 2010: Nature 413:39–44. 3. Aylett CHS, Wang Q, Michie KA, Amos LA, Löwe J(2010) Filament structure of bacterial tubulin homo-logue TubZ.
  21. Electron Cryo-microscopy - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/news-events/public-engagement/cryo-electron-microscopy/feed/
    2 Jul 2024: A thermostable, closed SARS-CoV-2 spike protein trimer./a Xiong, X., Qu, K., Ciazynska, KA., Hosmillo, M., Carter, AP., Ebrahimi, S., Ke, Z., Scheres, SHW., Bergamaschi, L., Grice, GL., Zhang,
  22. PowerPoint Presentation

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/ITC/Calorimetry%20Tutorial.ppt_files/Calorimetry%20Tutorial.ppt.ppt
    4 Dec 2007: Also recall that: KD = k-1 / k1 = [P]free [L]free/ [PL] = 1 / KA. ... y = [PL] / ([P]free+ [PL]) • Use [PL] = KA [P]free [L]free. •
  23. pnas201205508 15259..15264

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22949682.pdf
    25 Nov 2012: Full lipid-binding curves used tocalculate Ka values are in Fig. S7. ... B) Protein–lipid FRET assays were per-formed with 5% PIP2 vesicles, and Ka was calculated for both 5 μM pY.
  24. PII: S1097-2765(02)00515-4

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf
    19 Apr 2002: tide linker had little or no effect on its ability to bindstate affinity measurements to estimate on rate (ka), offrate (kd), and affinity (KA ka/kd) constants by measuring Scc1 (Figure ... dissociation rate constants (ka and kd, respectively) are
  25. pone.0107211 1..10

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf
    3 Sep 2014: Citation: Michie KA, Boysen A, Low HH, Møller-Jensen J, Löwe J (2014) LeoA, B and C from Enterotoxigenic Escherichia coli (ETEC) Are Bacterial Dynamins. ... 24. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes.
  26. Supplemental Materials Supp MethodsSupp Fig. 1 Sequence conservation…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/AP2Hub_EMBO2004/AP2Praefcke2004Sup.pdf
    23 Nov 2004: fitted to the data using the ORIGIN program supplied by themanufacturer yielding the stoichiometry N, the binary equilibrium constant Ka (= Kd-1)and the enthalpy of binding. ... Peptide binding to a-adaptin wild-type appendagePeptide Sequence Kd(mM)
  27. doi:10.1016/j.neuron.2005.05.004

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Jockusch_Neuron2005.pdf
    17 Jun 2005: XR r 1)2 4XR)1/2)) V, with XR = [LT]/[MT] and r = 1/KA[MT](Wiseman et al., 1989). ... This fit optimizes parameters of stoichiom-etry (N), association constant (KA), and the enthalpy of binding (H).The dissociation constant (KD) is the inverse of KA.
  28. Structure and superorganization of acetylcholinereceptor–rapsyn…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf
    3 Jul 2013: J Biol Chem 276(27):24911–24917. 6. Huebsch KA, Maimone MM (2003) Rapsyn-mediated clustering of acetylcholine re-ceptor subunits requires the major cytoplasmic loop of the receptor subunits.J
  29. The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf
    28 Nov 2008: Titration curves were fitted to the data using the ORIGIN program (MicroCal Inc.) which yielded the stoichiometry (1), the binary association constant Ka (=Kd -1) and the enthalpy of binding. ... The entropy of binding (S) was calculated from the
  30. doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf
    23 Dec 2013: Chem. 286:11756 –11764. 48. Burton BM, Marquis KA, Sullivan NL, Rapoport TA, Rudner DZ.
  31. Cryo‐EM structure of the full‐length Lon protease from Thermus…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf
    14 Nov 2021: aerolysin family protein. Nat Commun 7, 11293. 23 Zheng Shawn Q, Palovcak E, Armache J-P, Verba KA,.
  32. Reconstitution of a prokaryotic minus end-trackingsystem using TubRC…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2015-Fink-E1845-50.pdf
    27 Apr 2015: Mol Microbiol 67(4):881–890. 15. Aylett CH, Wang Q, Michie KA, Amos LA, Löwe J (2010) Filament structure of bacterialtubulin homologue TubZ.
  33. CELL AND MOLECULAR PHYSIOLOGY Intracellular Ca2+ release via the ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/44_Ong_HL_Pflug_Arch_2007.pdf
    15 Feb 2007: J Neurochem 67:1735–1743. 25. Roos J, DiGregorio PJ, Yeromin AV, Ohlsen K, Lioudyno M,Zhang S, Safrina O, Kozak JA, Wagner SL, Cahalan MD,Velicelebi G, Stauderman KA (2005) ... Biochem J289 (Pt 1):71–79. 36. Zhang SL, Yu Y, Roos J, Kozak JA, Deerinck
  34. The Jour nal o f Cel l Bio logy ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/epsinR.pdf
    9 Jan 2003: Titration curves were fitted to the data using the ORIGINprogram (MicroCal, Inc.) yielding the stoichiometry N, the binary equilib-rium constant KA ( KD 1) and the enthalpy of binding.
  35. 16262551036386 1..32

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cohesin%20folding%202021%20elife-67268-v1.pdf
    14 Jul 2021: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 28. Received:
  36. doi:10.1016/j.biocel.2008.08.010

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cytomotive2008Lowe.pdf
    17 Sep 2008: J Bacteriol 2006;188:469–76. ichie KA, Löwe J. Dynamic filaments of the bacterial cytoskeleton.
  37. 740 Research Paper Calcium triggers calcineurin-dependent synaptic…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Marks_McMahon1998.pdf
    21 Mar 2003: 740 Research Paper. Calcium triggers calcineurin-dependent synaptic vesicle recyclingin mammalian nerve terminalsBruno Marks and Harvey T. McMahon. Background: Following exocytosis at the synapse, synaptic vesiclecomponents are recovered by
  38. Interaction of tau protein with the dynactincomplex Enrico…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBOJ2007-Magnani-Fan.pdf
    15 Oct 2007: Proc Natl AcadSci USA 101: 6746–6751. Melkonian KA, Maier KC, Godfrey JE, Rodgers M, Schroer TA (2007)Mechanism of dynamitin-mediated disruption of dynactin.
  39. Membrane Localization is Critical for Activation of the PICK1 BAR…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf
    18 Oct 2008: # 2008 The Authors. Journal compilation # 2008 Blackwell Publishing Ltd. doi: 10.1111/j.1600-0854.2008.00761.xTraffic 2008; 9: 1327–1343Blackwell Munksgaard. Membrane Localization is Critical for Activationof the PICK1 BAR Domain. Kenneth L.
  40. bic296a

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/EberthBJ4170371.pdf
    24 Jan 2009: Binding parameters such asnumber of binding sites (n), the association constant (Ka) and thebinding enthalpy (H) were determined as parameters ofthe fitted experimental binding values.
  41. elifesciences.org Szwedziak et al. eLife 2014;3:e04601. DOI:…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsZ_Piotr_eLife_2014.pdf
    31 Dec 2014: elifesciences.org. Szwedziak et al. eLife 2014;3:e04601. DOI: 10.7554/eLife.04601 1 of 22. Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell divisionPiotr Szwedziak†, Qing Wang†, Tanmay A M Bharat, Matthew Tsim, Jan
  42. plbi-04-09-03 1532..1548

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/SchmidBetaPaper/BetaHub.pdf
    31 Aug 2006: Role of the AP2 b-Appendage Hubin Recruiting Partners for Clathrin-CoatedVesicle AssemblyEva M. Schmid. 1[, Marijn G. J. Ford. 1[, Anne Burtey. 2, Gerrit J. K. Praefcke. 3, Sew-Yeu Peak-Chew. 1, Ian G. Mills. 4,. Alexandre Benmerah2. , Harvey T.
  43. httDownloaded from rsif.royalsocietypublishing.org ResearchCite this…

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-1.pdf
    4 Feb 2015: httDownloaded from. rsif.royalsocietypublishing.org. ResearchCite this article: Butler VJ, Branicky R, YeminiE, Liewald JF, Gottschalk A, Kerr RA, Chklovskii. DB, Schafer WR. 2015 A consistent muscle. activation strategy underlies crawling and.
  44. 30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf
    1 May 2004: Cell Biol.13:55–60. 36. Erickson HP, Taylor DW, Taylor KA,Bramhill D. 1996.
  45. GB-9-7-229-Amos_9.qxd

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/gb-2008-9-7-229.pdf
    4 Aug 2008: Curr Biol 2005, 1155::R514-R526. 40. Michie KA, Löwe J: DDyynnaammiicc ffiillaammeennttss ooff tthhee bbaacctteerriiaall ccyyttoosskkeelleettoonn.Annu Rev Biochem 2006, 7755::467-492.
  46. pnas201313978 4601..4610

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas2013duman.pdf
    23 Dec 2013: Structural and genetic analyses reveal the proteinSepF as a new membrane anchor for the Z ringRamona Dumana,1, Shu Ishikawab,1, Ilkay Celikc,1, Henrik Strahlc, Naotake Ogasawarab, Paulina Troca, Jan Löwea,2,and Leendert W. Hamoenc,d,2. aMedical
  47. 2077 741..748

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/21_Lingappa_VR_BioEssays_2002.pdf
    5 Jul 2002: Dill KA, Chan HS. From Levinthal to pathways to funnels. Nat Struc Biol.
  48. The EMBO Journal Vol.17 No.18 pp.5273–5285, 1998 Crystal structure ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1998.pdf
    9 Sep 1998: Thecurves were fitted to a single exponential equationa exp(–kt) 1 c, from which the apparent initial rates(–ka) were extracted.
  49. The structural basis of novel endosome anchoringactivity of KIF16B ...

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17641687.pdf
    23 Jul 2007: The structural basis of novel endosome anchoringactivity of KIF16B kinesin. Nichole R Blatner1, Michael I Wilson2,Cai Lei3, Wanjin Hong3, Diana Murray4,Roger L Williams2 and Wonhwa Cho 1,1Department of Chemistry, University of Illinois at Chicago,
  50. pnas201210899 16522..16527

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2012-Aylett-16522-7.pdf
    12 Oct 2012: Proc Natl Acad Sci USA 107:11763–11768. 14. Aylett CHS, Wang Q, Michie KA, Amos LA, Löwe J (2010) Filament structure of bac-terial tubulin homologue TubZ.
  51. Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Szewczak-Harris_et_al-2019-FEBS_Letters.pdf
    22 Jul 2019: EDITOR’S CHOICE. Cryo-EM structure of the MinCD copolymeric filamentfrom Pseudomonas aeruginosa at 3.1 A resolutionAndrzej Szewczak-Harris , James Wagstaff and Jan Lowe. MRC Laboratory of Molecular Biology, Cambridge, UK. Correspondence. J. Lowe,

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