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publications | The Bacterial Cytoskeleton and other Molecular Machines
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/page-4/index.html29 Apr 2024: Jan Löwe's group at the MRC Laboratory of Molecular Biology. Jan Löwe's ORCID identifier: 0000-0002-5218-6615. For statistics such as h-index and citation numbers please consult Google Scholar: Jan Löwe's profile. 2024. Hale V., Hooker J., Russo C -
Published Research - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/research/published-research/21 Jul 2023: Nature 630(8017): 596-608. Prentice, A., Jarjou, LM., Goldberg, GR., Schoenbuchner, SM., Moore, SE., Ward, KA., Cole, TJ. -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/1018
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/1018 -
Publications
https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/publications/AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O’Connor-Giles KM, Parks AL, Perrimon N, Port F, Russell S, Ueda R, Wildonger J (2015) Biosafety: Safeguarding gene drive experiments -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/585452
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/585452{"id":585452,"date":"2023-11-28T10:54:06","date_gmt":"2023-11-28T10:54:06","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?p=585452"},"modified":"2023-12-11T12:23:41","modified_gmt":"2023-12-11T12:23:41","slug":"low-energy-cryo-em-to-widen-access -
full Publications
https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/publications/full_publications/MM, Hayes KR, James AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O’Connor-Giles KM, Parks AL, Perrimon N, Port F, Russell S, Ueda R, Wildonger J (2015) Biosafety: -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/18964
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/18964{"id":18964,"date":"2020-08-18T14:05:27","date_gmt":"2020-08-18T13:05:27","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?p=18964"},"modified":"2022-03-21T15:56:30","modified_gmt":"2022-03-21T15:56:30","slug":"studying-the-spike-protein-of-sars-c -
16001658465475 1..36
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Collier_2020_elife-59560-v1.pdf15 Sep 2020: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 33. Received: -
Studying the spike protein of SARS-CoV-2 - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/studying-the-spike-protein-of-sars-cov-2/18 Aug 2020: Xiong, X., Qu, K., Ciazynska, KA., Hosmillo, M., Carter, AP., Ebrahimi, S., Ke, Z., Scheres, SHW., Bergamaschi, L., Grice, GL., Zhang, Y., The CITIID-NIHR COVID-19 BioResource Collaboration, Nathan, JA., -
https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/29
https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/29KG, Gratz SJ, Harrison MM, Hayes KR, James AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O’Connor-Giles KM, Parks AL, Perrimon N, Port F, Russell S, Ueda R, -
Dlp Purification:
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Eva%20Methods.htm6 Feb 2008: Affinities were calculated via fitting titration curves to the data. This resulted in the stoichiometry N, the binary equilibrium constant Ka, the dissociation constant Kd (which equals 1/Ka), the enthalpy -
373_431_BIOsp_0411
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Biospektrum2011%20Gasper.pdf1 Jul 2011: Int JBiochem Cell Biol 41:323–329[3] Aylett CH, Wang Q, Michie KA et al. ... Cell 115:705–713[12] Michie KA, Löwe J (2006) Dynamic filaments of the bac-terial cytoskeleton. -
Low energy cryo-EM to widen accessibility for fast and accurate…
https://www2.mrc-lmb.cam.ac.uk/low-energy-cryo-em-to-widen-accessibility-for-fast-and-accurate-structure-determination/28 Nov 2023: McMullan, G., Naydenova, K., Mihaylov, D., Yamashita, K., Peet, MJ., Wilson, H., Dickerson, JL., Chen, S., Cannone, G., Lee, Y., Hutchings, KA., Gittins, O., Sobhy, MA., Wells, T., El-Gomati, MM., -
https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/58
https://www2.mrc-lmb.cam.ac.uk/groups/sbullock/wp-json/wp/v2/pages/58KR, Duchek P, Edwards OR, Esvelt K, Gantz VM, Golic KG, Gratz SJ, Harrison MM, Hayes KR, James AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O’Connor-Giles KM, -
Contrasting Membrane Interaction Mechanisms of AP180N-terminal…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Stahelin_cho2003.pdf16 Jul 2003: smaller ka) but prolonged its membrane residence by3-fold (i.e. smaller kd). ... Proteins ka kd kdFold increase. in Kda. M 1 s 1 s 1 M. -
Public Engagement - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/news-events/public-engagement/feed/2 Jul 2024: A thermostable, closed SARS-CoV-2 spike protein trimer./a Xiong, X., Qu, K., Ciazynska, KA., Hosmillo, M., Carter, AP., Ebrahimi, S., Ke, Z., Scheres, SHW., Bergamaschi, L., Grice, GL., Zhang, -
2168 | K. von der Malsburg et al. Molecular ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf11 Aug 2015: 2168 | K. von der Malsburg et al. Molecular Biology of the Cell. MBoC | ARTICLE. The ribosome quality control pathway can access nascent polypeptides stalled at the Sec61 transloconKarina von der Malsburg, Sichen Shao, and Ramanujan S. HegdeMRC -
cdf519 5057..5068
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12356722.pdf12 Oct 2002: The enhanced membrane afnity of the p47phox-PXdomain due to 3 mol% PtdIns(3,4)P2 is almost entirely dueto a decrease in the kd, with the ka remaining nearlyconstant. ... Phosphoinositide ka (M1 s1) kd (s. 1) Kd (M) Fold increase in Kda. -
pnas201014665 19609..19610
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Nogales-19609-10.pdf11 Nov 2010: Nature 413:39–44. 3. Aylett CHS, Wang Q, Michie KA, Amos LA, Löwe J(2010) Filament structure of bacterial tubulin homo-logue TubZ. -
Electron Cryo-microscopy - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/news-events/public-engagement/cryo-electron-microscopy/feed/2 Jul 2024: A thermostable, closed SARS-CoV-2 spike protein trimer./a Xiong, X., Qu, K., Ciazynska, KA., Hosmillo, M., Carter, AP., Ebrahimi, S., Ke, Z., Scheres, SHW., Bergamaschi, L., Grice, GL., Zhang, -
PowerPoint Presentation
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/ITC/Calorimetry%20Tutorial.ppt_files/Calorimetry%20Tutorial.ppt.ppt4 Dec 2007: Also recall that: KD = k-1 / k1 = [P]free [L]free/ [PL] = 1 / KA. ... y = [PL] / ([P]free+ [PL]) • Use [PL] = KA [P]free [L]free. • -
pnas201205508 15259..15264
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22949682.pdf25 Nov 2012: Full lipid-binding curves used tocalculate Ka values are in Fig. S7. ... B) Protein–lipid FRET assays were per-formed with 5% PIP2 vesicles, and Ka was calculated for both 5 μM pY. -
PII: S1097-2765(02)00515-4
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf19 Apr 2002: tide linker had little or no effect on its ability to bindstate affinity measurements to estimate on rate (ka), offrate (kd), and affinity (KA ka/kd) constants by measuring Scc1 (Figure ... dissociation rate constants (ka and kd, respectively) are -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: Citation: Michie KA, Boysen A, Low HH, Møller-Jensen J, Löwe J (2014) LeoA, B and C from Enterotoxigenic Escherichia coli (ETEC) Are Bacterial Dynamins. ... 24. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes. -
Supplemental Materials Supp MethodsSupp Fig. 1 Sequence conservation…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/AP2Hub_EMBO2004/AP2Praefcke2004Sup.pdf23 Nov 2004: fitted to the data using the ORIGIN program supplied by themanufacturer yielding the stoichiometry N, the binary equilibrium constant Ka (= Kd-1)and the enthalpy of binding. ... Peptide binding to a-adaptin wild-type appendagePeptide Sequence Kd(mM) -
doi:10.1016/j.neuron.2005.05.004
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Jockusch_Neuron2005.pdf17 Jun 2005: XR r 1)2 4XR)1/2)) V, with XR = [LT]/[MT] and r = 1/KA[MT](Wiseman et al., 1989). ... This fit optimizes parameters of stoichiom-etry (N), association constant (KA), and the enthalpy of binding (H).The dissociation constant (KD) is the inverse of KA. -
Structure and superorganization of acetylcholinereceptor–rapsyn…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf3 Jul 2013: J Biol Chem 276(27):24911–24917. 6. Huebsch KA, Maimone MM (2003) Rapsyn-mediated clustering of acetylcholine re-ceptor subunits requires the major cytoplasmic loop of the receptor subunits.J -
The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf28 Nov 2008: Titration curves were fitted to the data using the ORIGIN program (MicroCal Inc.) which yielded the stoichiometry (1), the binary association constant Ka (=Kd -1) and the enthalpy of binding. ... The entropy of binding (S) was calculated from the -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: Chem. 286:11756 –11764. 48. Burton BM, Marquis KA, Sullivan NL, Rapoport TA, Rudner DZ. -
Cryo‐EM structure of the full‐length Lon protease from Thermus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf14 Nov 2021: aerolysin family protein. Nat Commun 7, 11293. 23 Zheng Shawn Q, Palovcak E, Armache J-P, Verba KA,. -
Reconstitution of a prokaryotic minus end-trackingsystem using TubRC…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2015-Fink-E1845-50.pdf27 Apr 2015: Mol Microbiol 67(4):881–890. 15. Aylett CH, Wang Q, Michie KA, Amos LA, Löwe J (2010) Filament structure of bacterialtubulin homologue TubZ. -
CELL AND MOLECULAR PHYSIOLOGY Intracellular Ca2+ release via the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/44_Ong_HL_Pflug_Arch_2007.pdf15 Feb 2007: J Neurochem 67:1735–1743. 25. Roos J, DiGregorio PJ, Yeromin AV, Ohlsen K, Lioudyno M,Zhang S, Safrina O, Kozak JA, Wagner SL, Cahalan MD,Velicelebi G, Stauderman KA (2005) ... Biochem J289 (Pt 1):71–79. 36. Zhang SL, Yu Y, Roos J, Kozak JA, Deerinck -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/epsinR.pdf9 Jan 2003: Titration curves were fitted to the data using the ORIGINprogram (MicroCal, Inc.) yielding the stoichiometry N, the binary equilib-rium constant KA ( KD 1) and the enthalpy of binding. -
16262551036386 1..32
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cohesin%20folding%202021%20elife-67268-v1.pdf14 Jul 2021: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 28. Received: -
doi:10.1016/j.biocel.2008.08.010
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cytomotive2008Lowe.pdf17 Sep 2008: J Bacteriol 2006;188:469–76. ichie KA, Löwe J. Dynamic filaments of the bacterial cytoskeleton. -
740 Research Paper Calcium triggers calcineurin-dependent synaptic…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Marks_McMahon1998.pdf21 Mar 2003: 740 Research Paper. Calcium triggers calcineurin-dependent synaptic vesicle recyclingin mammalian nerve terminalsBruno Marks and Harvey T. McMahon. Background: Following exocytosis at the synapse, synaptic vesiclecomponents are recovered by -
Interaction of tau protein with the dynactincomplex Enrico…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBOJ2007-Magnani-Fan.pdf15 Oct 2007: Proc Natl AcadSci USA 101: 6746–6751. Melkonian KA, Maier KC, Godfrey JE, Rodgers M, Schroer TA (2007)Mechanism of dynamitin-mediated disruption of dynactin. -
Membrane Localization is Critical for Activation of the PICK1 BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf18 Oct 2008: # 2008 The Authors. Journal compilation # 2008 Blackwell Publishing Ltd. doi: 10.1111/j.1600-0854.2008.00761.xTraffic 2008; 9: 1327–1343Blackwell Munksgaard. Membrane Localization is Critical for Activationof the PICK1 BAR Domain. Kenneth L. -
bic296a
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/EberthBJ4170371.pdf24 Jan 2009: Binding parameters such asnumber of binding sites (n), the association constant (Ka) and thebinding enthalpy (H) were determined as parameters ofthe fitted experimental binding values. -
elifesciences.org Szwedziak et al. eLife 2014;3:e04601. DOI:…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsZ_Piotr_eLife_2014.pdf31 Dec 2014: elifesciences.org. Szwedziak et al. eLife 2014;3:e04601. DOI: 10.7554/eLife.04601 1 of 22. Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell divisionPiotr Szwedziak†, Qing Wang†, Tanmay A M Bharat, Matthew Tsim, Jan -
plbi-04-09-03 1532..1548
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/SchmidBetaPaper/BetaHub.pdf31 Aug 2006: Role of the AP2 b-Appendage Hubin Recruiting Partners for Clathrin-CoatedVesicle AssemblyEva M. Schmid. 1[, Marijn G. J. Ford. 1[, Anne Burtey. 2, Gerrit J. K. Praefcke. 3, Sew-Yeu Peak-Chew. 1, Ian G. Mills. 4,. Alexandre Benmerah2. , Harvey T. -
httDownloaded from rsif.royalsocietypublishing.org ResearchCite this…
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-1.pdf4 Feb 2015: httDownloaded from. rsif.royalsocietypublishing.org. ResearchCite this article: Butler VJ, Branicky R, YeminiE, Liewald JF, Gottschalk A, Kerr RA, Chklovskii. DB, Schafer WR. 2015 A consistent muscle. activation strategy underlies crawling and. -
30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf1 May 2004: Cell Biol.13:55–60. 36. Erickson HP, Taylor DW, Taylor KA,Bramhill D. 1996. -
GB-9-7-229-Amos_9.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/gb-2008-9-7-229.pdf4 Aug 2008: Curr Biol 2005, 1155::R514-R526. 40. Michie KA, Löwe J: DDyynnaammiicc ffiillaammeennttss ooff tthhee bbaacctteerriiaall ccyyttoosskkeelleettoonn.Annu Rev Biochem 2006, 7755::467-492. -
pnas201313978 4601..4610
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas2013duman.pdf23 Dec 2013: Structural and genetic analyses reveal the proteinSepF as a new membrane anchor for the Z ringRamona Dumana,1, Shu Ishikawab,1, Ilkay Celikc,1, Henrik Strahlc, Naotake Ogasawarab, Paulina Troca, Jan Löwea,2,and Leendert W. Hamoenc,d,2. aMedical -
2077 741..748
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/21_Lingappa_VR_BioEssays_2002.pdf5 Jul 2002: Dill KA, Chan HS. From Levinthal to pathways to funnels. Nat Struc Biol. -
The EMBO Journal Vol.17 No.18 pp.5273–5285, 1998 Crystal structure ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1998.pdf9 Sep 1998: Thecurves were fitted to a single exponential equationa exp(–kt) 1 c, from which the apparent initial rates(–ka) were extracted. -
The structural basis of novel endosome anchoringactivity of KIF16B ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17641687.pdf23 Jul 2007: The structural basis of novel endosome anchoringactivity of KIF16B kinesin. Nichole R Blatner1, Michael I Wilson2,Cai Lei3, Wanjin Hong3, Diana Murray4,Roger L Williams2 and Wonhwa Cho 1,1Department of Chemistry, University of Illinois at Chicago, -
pnas201210899 16522..16527
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2012-Aylett-16522-7.pdf12 Oct 2012: Proc Natl Acad Sci USA 107:11763–11768. 14. Aylett CHS, Wang Q, Michie KA, Amos LA, Löwe J (2010) Filament structure of bac-terial tubulin homologue TubZ. -
Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Szewczak-Harris_et_al-2019-FEBS_Letters.pdf22 Jul 2019: EDITOR’S CHOICE. Cryo-EM structure of the MinCD copolymeric filamentfrom Pseudomonas aeruginosa at 3.1 A resolutionAndrzej Szewczak-Harris , James Wagstaff and Jan Lowe. MRC Laboratory of Molecular Biology, Cambridge, UK. Correspondence. J. Lowe,
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