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  2. Carter lab dynactin prep from frozen porcine brains, using ...

    https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/2020_Carter_dynactin_brain_prep.docx
    28 Feb 2020: Overnight, wash the G4000 from storage in 20% EtOH to water (2 CV). ... Stored in. SP-sepharose. 250. 0.3. 4. 20% EtOH. MonoQ 16/10. 20.
  3. Carter lab insect cell ZZ-tag prep (2020) Buffers Wash ...

    https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_Sf9_dynein_prep.docx
    24 Jan 2020: Gel-filtration on AKTAmicro. -Column is stored in water 0.05% NaN3. If stored in 20% ethanol, first wash into water before washing into buffer. ... Remove column and replace caps on either end. -Put inlet A1 into 20% ethanol, pumpwash.
  4. 31/10/2018 Carter lab TIRF microscopy Single Molecule Assay 5x ...

    https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_TIRF_protocol.docx
    28 Jan 2020: 15 mg Glucose Oxidase 2mg Catalase (both in -20) into 250 μL DLB. ... Don’t pipette up and down. Could use a cut 20 μL tip for this.

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