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1 - 10 of 33 search results for TALK:PC53 20 |u:www2.mrc-lmb.cam.ac.uk where 0 match all words and 33 match some words.

  1. Results that match 1 of 2 words

  2. Barbara Pearseís method for prepping CCVs

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/CCVprep.html
    25 Jun 2007: Mix by inversion to ensure homogeneity. 11. Spin in a 70Ti rotor at 25000rpm for 20 minutes. ... Run at 1ml/min (Abs. 0.5) and collect 2ml fractions. Clathrin elutes at around fraction 20.

  3. Materials and Methods

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/BARdomains/methods.html
    25 Jun 2007: Crystals were equilibrated in 20% glycerol for cooling to 100K. The crystal asymmetric unit contains one molecule, and the crystals belong to spacegroup P3121, cell dimensions a = b = 49.6Å, c = ... Sedimentation was at 11,000 rev/min, 20.0C, with
    By: default

  4. Liposome and Lipid tubule Production

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Liposome.html
    25 Jun 2007: NB: the filtration process usually leads to loss of around 100-200 ul of liquid, and 10-20% of the lipid in the reamining volume.

  5. Effects of Epsin R overexpression

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/epsinrL10E.html
    25 Jun 2007: EpsinR L10E overexpression in COS cells. Myc-EpsinR L10E with endogenous AP1 and internalised transferrin in COS cells. Insertion of a glutamic acid (E) in place of the hydrophobic leucine (L) is predicted to affect epsinR's ability to bend membranes
    By: default

  6. Negative-staining protocol for EM: Harvey McMahon lab

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Negative%20stain%20protocol.html
    25 Jun 2007: Usually I mix at least 20 ul; otherwise evaporation can drastically change the salt content. ... Pipette drops of UrAc (20-40 ul each) onto ParafilmÑtwo drops per sample.
    By: Word.Document

  7. Monolayer Assay Protocol

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/mono.html
    25 Jun 2007: Purified clathrin. Clathrin should be dialyzed into HKM buffer and centrifuged for 20 minutes at 100000 gmax (e.g., 45000 r.p.m.

  8. Alonsoetal Supplementary Material 030107

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso-suppmat.pdf
    2 Apr 2007: Purified tubulins were desalted into PEM containing 20 μM GDP before storage in liquid nitrogen. ... Enzyme reactions were done in 20 mM PIPES pH 6.9, 2 mM MgCl2, using quartz fluorimeter cuvettes.
    By: rob cross

  9. DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf
    6 Apr 2007: 0 5 10 15 20. 0 5 10 15 20. 0 5 10 15 20-10. ... Themicrotubule plus end is toward the top of thepage. (Left) Cryo-EM map (20).

  10. Prel progr with speakers

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/F-BAR_proteins/Prog_PCH_conference.pdf
    25 Jul 2007: 18:30 Dinner 20:00 Opening event: Concert at Schloß Waldthausen by „Trio con brio“ (piano trio) 22:00 Get together in the „Weinkeller“ of Schloß Waldthausen Thursday, Oct 11th, 2007
    By: Ann

  11. struct1345mmc1

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BAR_SUP07.pdf
    2 Jun 2007: Samples at different. loading concentrations were sedimented at 10,000 rpm at 20.0 ºC in double sector cells.
    By: coconnor

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