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3 Jun 2005: add 50 mM Tris pH 8 up to 2L, 20 EDTA-free PI tablets (Roche). ... follow your usual elution protocol, everything at 20 ml/min. JL 3/6/2005.

21 Jun 2005: and size-exclusion chromatography (S300 Sephacryl,Amersham Pharmacia; 20 mM TrisHCl1 mM azide1 mMEDTA, pH 7.5). ... After 30 min at room temperature, samples werecentrifuged at 80,000 rpm for 20 min at 20C in a TLA100 rotor(Beckman).

9 Sep 2005: Pressure reading on line 15 shoud be 20-30 psi. Any valve when open should have a small plug popped-out.

19 Jan 2005: At the MRC Laboratory of Molecular Biology (LMB) about 15–20 groups are more or lessintensely involved in protein crystallisation and/or structure determination. ... Our initial idea for long-term storage of the plateswas to store them at 20 1C to

5 Jan 2005: dendrites. Tuba [20], anotherBAR protein, has Rho GEF activity and multiple SH3 domains that binddynamin, N-WASP (neural Wiskott–Aldrich syndrome protein) and otheractin-regulating proteins.

30 Jan 2005: Soj ATPase is activated by the N-terminal 20 amino. acids of Spo0J. ... MinE: first 35 amino acids (N-terminal domain). FtsA:last 20 amino acids.

3 Dec 2005: The onset detection algorithm was tested on 25 videos of 20-minute recordings. ... previously used for the egg detection test and 20 new normal wild type videos.

22 Mar 2005: and C.L.,acid, 20 mM HEPES [pH 7.0], adjusted to 300 mosM with NaCl, and. ... After washing, excess MESNA was port. J. Biol. Chem. 279, 4175–4179.quenched with 150 mM iodoacetamide for 20 min.

4 Oct 2005: 20. signaling pathways in the vulval muscles. In the future, the identification and characterization. ... Wild-. type animals were grown at 20 C; approximately 10 hours after hatching, the Pn.a cell nuclei.

17 Jun 2005: Cl, 0.4 mM BAPTA, and 20 mM HEPES (pH 7.2, 260 mOsm/kg1). ... aining 20 mM HEPES (pH 7.4), 150 mM NaCl, 1 mM DTT, 0.1%riton, and 0.1% protease inhibitors (Sigma).