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doi:10.1016/S1097-2765(03)00246-6
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12887891.pdf5 Sep 2003: to-back interacting PB1 domains, with a freebuffer C (20 mM Tris, pH 7.4, 100 mM NaCl, and 5 mM DTT). ... C.D., Anderson, K.E., Butler, P.J., Lavenir, I., Perisic, O., Hawkins,GST/Par6b binding assays) and run on 20% Phast SDS gels. -
PII: S1097-2765(03)00356-3
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/gripdomain.pdf27 Oct 2003: being represented by 3 of the 20 ESTs for human golgin-(C) The oligomerization state of the His6-GRIP domain derived by 245 in the current GenBank database. ... The complex was concentrated ton X-100 in PBS, blocked with 20% (v/v) fetal calf serum/0.25%. -
PII: 0896-6273(89)90235-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/neuron89.pdf11 Sep 2003: Usually there are four or more stretches of about 20 hydrophobic amino acids per subunit or domain: the correct length for an a-helix to span the hydrophobic lipid chains. ... the contacting helices are inclined to one another by even greater angles: -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/24_Fons_RD_JCB_2003.pdf6 Feb 2003: factor inthe same proteoliposomes was only. 15–20% of the control,raising the possibility that its transport may require stimula-tory factor(s) not needed by Prl. ... Only after the synthesis of an additional 20 resi-dues did the PrP–Prl intermediate -
554 Research Paper Inhibition of receptor-mediated endocytosis by the …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf?cmd=Retrieve&db=PubMed&list_uids=9348539&dopt=Abstract21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se, -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/26_Snapp_EL_JCB_2003.pdf16 Oct 2003: The. Jour. nal o. f Cel. l Bio. logy. JCB. Article. The Journal of Cell Biology, Volume 163, Number 2, October 27, 2003 257–269http://www.jcb.org/cgi/doi/10.1083/jcb.200306020 257. Formation of stacked ER cisternae by low affinity protein
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