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SEC-MALS_sample_preparation_031121
https://facilities.bioc.cam.ac.uk/files/media/sec-mals_sample_preparation_031121.pdf9 Nov 2021: for proteins of 50-60 kDa, 3 mg/ml if MW is closer to 20 kDa and 1.5 mg/ml if 100 kDa or more. -
ITC_sample_preparation_310713
https://facilities.bioc.cam.ac.uk/files/media/itc_sample_preparation_310713.pdf31 Jan 2021: of ligand for syringe (40 µL syringe 20 µL for filling) Typical starting concentrations are 10-50 µM for the cell and 50-500 µM for the syringe. ... 10-20 for iTC200) to ensure an S-shaped titration curve with a plateau. -
SOP for the SX19 v3
https://facilities.bioc.cam.ac.uk/files/media/sop_for_the_sx19_v3.pdf17 Mar 2021: Leave it filled with 80:20 water:ethanol when not in use. 3. ... Final wash. Finish by replacing the reservoir syringes with ones containing clean purified water and 20% ethanol. -
4140Q010_SX_Enzyme Kinetics App Note_v 1.2.indd
https://facilities.bioc.cam.ac.uk/files/media/enzyme_kinetics_app_note_final.pdf1 Feb 2021: All chemicals and reagents were purchased from Sigma-Aldrich. 60μM α-chymotrypsinin (20% Isopropyl alcohol, 20mM Phosphate bu er, pH7.3) was mixed with p-Nitrophenolate (250μM to 8mM, 20% Isopropyl -
Dynamic light scattering common terms defined
https://facilities.bioc.cam.ac.uk/files/media/dls_terms_defined_malvern.pdf1 Feb 2021: In terms of a protein analysis, a % polydispersity less than 20% indicates that the sample is monodisperse. -
SEC-MALS_sample_preparation_031121
https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/sec-mals_sample_preparation_031121.pdf9 Nov 2021: for proteins of 50-60 kDa, 3 mg/ml if MW is closer to 20 kDa and 1.5 mg/ml if 100 kDa or more. -
ITC_sample_preparation_310713
https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/itc_sample_preparation_310713.pdf31 Jan 2021: of ligand for syringe (40 µL syringe 20 µL for filling) Typical starting concentrations are 10-50 µM for the cell and 50-500 µM for the syringe. ... 10-20 for iTC200) to ensure an S-shaped titration curve with a plateau. -
Selection guide Biacore consumables 2 No matter what you ...
https://facilities.bioc.cam.ac.uk/files/media/16208_10061.pdf17 Mar 2021: Reagent Rack, type 1. For Biacore S200, and Biacore T200. BR100481 20 11 mm BR100287 BR100411. ... BR100411. BR100411. BR100502. Reagent Rack C. For Biacore C. BR100413 20 7 mm BR100212 BR100502. -
Standard_operating_procedure_for_Aviv_410_CD_instrument
https://facilities.bioc.cam.ac.uk/files/media/standard_operating_procedure_for_aviv_410_cd_instrument.pdf31 Jan 2021: 3. Adjust the N2 flow regulator to 20. 4. Turn on the water-bath pump situated on the unit to the right of the CD. -
SOP for the SX19 v3
https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/sop_for_the_sx19_v3.pdf17 Mar 2021: Leave it filled with 80:20 water:ethanol when not in use. 3. ... Final wash. Finish by replacing the reservoir syringes with ones containing clean purified water and 20% ethanol.
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