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17 Jul 2005: found, the location of the knotted core can bepinpointed; “deep” knots have more than 20 aminoacid residues on either side of the core.17 Thealgorithm led to the identification of an ... consideredhere.20 To understand further the mechanismsinvolved

8 Feb 2008: Forlocalisation of hydrogen exchange in pepsin-digestedHsp90 samples, typical instrumental settings include:mass range 200–2000 Da, capillary voltage 1400 V andcone voltage 20 V. ... Cell,90, 65–75. 20. Stebbins, C. E., Russo, A. A., Schneider, C.,

8 Feb 2010: The yellow fractions were pooled and con-centrated (Vivaspins 20; Vivascience). The concentrated pro-tein (10 ml) was gel-filtrated on a HiLoad 26/60 SuperdexG-75 column (Amersham Biosciences), which ... concentration of 140 M and buffered in 20 mM

15 Sep 2003: Ala27 1.97 0.17 ÿ6.40 0.20 0.99 0.03 ÿ2.56 0.09 0.38 0.06 0.28 0.03Thr! ... Asp56 3.16 0.20 ÿ3.54 0.10 0.76 0.02 ÿ0.60 0.03 0.08 0.01 0.18 0.01Arg!

28 Sep 2009: Fluorescent proteins have been denatured with acid,18–20. pressure,21 temperature,21 force22–26 and chemical. ... fluorescence, indicating that, for DsRed, the acid unfolding. was not fully reversible.20.

5 Jun 2006: Thomas, J. Mol. Biol., 2003, 327, 1135–1148.20 M. Sundd and A.

23 Aug 2004: their ideas to experimental data on fast folding proteins. [20]. Methods to generate representations of the energy sur-. ... as can be seen in Fig. 6. In the experimental study of ubiquitin, of the 20 /-valuescalculated, 8 have values greater than 0.5,

4 Jul 2006: Unfolding traces areshown after (c) 200 ms refolding delay, (d) 5 s refoldingdelay and (e) 20 s refolding delay. ... 360.02 1.30.1 2.50.14.5 10.12.1 1.40.2 1.20.3 0.420.02 1.60.3 1.20.1.

8 Feb 2008: 1; green, 7.0 kcal mol 1bblue, very fast (amide hydrogen exchanged within 20 min); gspectrum. ... time it takes to acquire the first spectrum (ca 20 min).If ΔGHX is greater than 12 kcal mol.

6 Sep 2005: 0.04 2.16 6 0.06 2.26 6 0.03 1.10 6 0.01 3.20 6 0.09 2.80 6 0.11Val!Ala17 1.65 6 ... 0.04 4.71 6 0.10 0.08 6 0.04 1.20 6 0.02 4.78 6 0.04 2.71 6 0.04Ile!Ala30 3.22 6